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Ni agarose columns

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Ni-agarose columns are a type of chromatography media used for the purification of proteins. They contain nickel-charged agarose resin that can selectively bind to proteins with a histidine tag, allowing for their efficient separation from other components in a sample.

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Lab products found in correlation

Quick start bradford dye reagent, by Bio-Rad (2 mentions)

2 protocols using ni agarose columns

1

Antibiotics and Molecular Biology Reagents

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The antibiotics used in this study were obtained from Sigma-Aldrich (St. Louis, MO, USA). TaKaRa Biotechnology Co. (Dalian, Liaoning, China) provided the molecular biology reagents, and Novagen (Madison, WI, USA) provided the pET vectors. Ni-agarose columns were obtained from Sigma-Aldrich, and Bio-Rad (Hercules, CA, USA) provided the Quick Start Bradford dye reagent. All other reagents used in this study were of the highest purity available. Genscript (Nanjing, Jiangsu, China) synthesized the oligonucleotide primers.
Li K., Hou R., Xu H., Wu G., Qian G., Wang H, & Liu F. (2020). Two Functional Fatty Acyl Coenzyme A Ligases Affect Free Fatty Acid Metabolism To Block Biosynthesis of an Antifungal Antibiotic in Lysobacter enzymogenes. Applied and Environmental Microbiology, 86(10), e00309-20.
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2

Bacterial Protein Expression Optimization

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The antibiotics were obtained from Sigma‐Aldrich. Takara Biotechnology Co. provided the molecular biology reagents. Novagen provided the pET vectors. Ni‐agarose columns were obtained from Sigma‐Aldrich. Bio‐Rad provided Quick Start Bradford dye reagent. All other reagents were of the highest available quality. Genscript (Nanjing, Jiangsu, China) synthesized the oligonucleotide primers.
Li K., Wu G., Liao Y., Zeng Q., Wang H, & Liu F. (2020). RpoN1 and RpoN2 play different regulatory roles in virulence traits, flagellar biosynthesis, and basal metabolism in Xanthomonas campestris. Molecular Plant Pathology, 21(7), 907-922.
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