Aspartate colorimetric assay kit

Manufactured by Abcam

The Aspartate Colorimetric Assay Kit is a laboratory tool used to measure the concentration of aspartate, an amino acid, in biological samples. The kit provides a colorimetric method for the quantitative determination of aspartate levels.

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Lab products found in correlation

Synergy ht, by Agilent Technologies (1 mentions) Enzychrom glutamate assay kit, by BioAssay Systems (1 mentions) Lactate colorimetric assay kit, by Abcam (1 mentions) Glucose colorimetric assay kit, by Abcam (1 mentions) Glutamine colorimetric assay kit, by Abcam (1 mentions) Glutamate colorimetric assay kit, by Abcam (1 mentions) Nad nadh assay kit, by Merck Group (1 mentions)

Spelling variants of this product

Colorimetric assay kit (281 citations) Colorimetric assay (66 citations) Aspartate Assay Kit (10 citations) Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit (2 citations) Aspartate Aminotransferase Activity Colorimetric Assay Kit (2 citations) Aspartate Colorimetric/Fluorometric Assay Kit (2 citations)

3 protocols using aspartate colorimetric assay kit

Quantifying Intracellular Aspartate in T Cells

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T cells were thawed and rested in pre-warmed RPMI 1640 medium containing 10% FBS for 3–4 hours at 37°C and 5% CO₂. To prepare lysates, rested T cells were washed twice with DPBS and resuspended in AST assay buffer (BioVision, Cat# K552). Lysates were homogenized with a Dounce homogenizer and subsequently deproteinated through 10 kDa Amicon filters. Intracellular aspartate was quantified using a plate-based Aspartate Colorimetric Assay Kit (BioVision, Cat# K552). Lysates (50 μL) were incubated for 30 minutes with the enzyme reaction mixture (50 μL). Absorbance was measured at an optical density of 570 nm and aspartate concentration was extrapolated from a standard curve following background subtraction.

Hickman T.L., Choi E., Whiteman K.R., Muralidharan S., Pai T., Johnson T., Parikh A., Friedman T., Gilbert M., Shen B., Barron L., McGinness K.E., Ettenberg S.A., Motz G.T., Weiss G.J, & Jensen-Smith A. (2022). BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS ONE, 17(5), e0266980.

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Quantifying Amino Acid Levels in Murine Femurs

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Femurs were harvested from 8-wk-old female C57BL/6J mice and frozen immediately on dry ice. Tissues were stored at −80 °C before being homogenized in 500 µL of PBS. Asp and Glu concentrations were measured by the Aspartate Colorimetric Assay Kit (BioVision) and EnzyChrom Glutamate Assay Kit (BioAssay Systems), respectively, according to manufacturer instructions. Absorbances were measured by a BioTek Synergy HT 96-well plate reader. Metabolite concentrations were normalized to wet tissue weight.

Potter A.D., Butrico C.E., Ford C.A., Curry J.M., Trenary I.A., Tummarakota S.S., Hendrix A.S., Young J.D, & Cassat J.E. (2020). Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection. Proceedings of the National Academy of Sciences of the United States of America, 117(22), 12394-12401.

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Metabolite Quantification by Commercial Kits

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The levels of selected metabolites were measured by commercial kits to confirm the results of metabolic profiling. These include the aspartate colorimetric assay kit (BioVision), the glutamate colorimetric assay kit (BioVision), the glutamine colorimetric assay kit (BioVision), the glucose colorimetric assay kit (BioVision), the lactate colorimetric assay kit (BioVision) the NAD+/NADH assay kit (Sigma-Aldrich), and the GSH/GSSG colorimetric assay kit (Abcam) The manufacturers’ protocols were followed. Cell number was determined in concurrent experiment run in parallel, averaged per condition, and the metabolite consumption/production rates were calculated per cell.

Bertero T., Oldham W.M., Grasset E.M., Bourget I., Boulter E., Pisano S., Hofman P., Bellvert F., Meneguzzi G., Bulavin D.V., Estrach S., Feral C.C., Chan S.Y., Bozec A, & Gaggioli C. (2018). Tumor-stroma mechanics coordinate amino acid availability to sustain tumor growth and malignancy. Cell metabolism, 29(1), 124-140.e10.

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