2 8 3h adenine

IGF-1, EGF, FSK, and noradrenalin were from Sigma-Aldrich (Taufkrichen, Germany). The SRF/TCF-dependent reporter was from Dr. Susanne Mühlich, Walter-Straub-Institute, LMU, München, Germany. Oligonucleotids (primer) were from Metabion, Martinsried, Germany. [2,8-³H]-adenine was from PerkinElmer (Boston, MA, USA). The anti-SDHA (ab14715) and the anti-IRS (ab131487) antibodies were from Abcam. The anti-SREBP-1 (sc-13551), anti-SREBP-2 (sc-13552), and the anti-ERK1 D2 (sc-1647) antibodies were from Santa Cruz. The anti-ADCY6 (PA5-101284) antibody was from Thermo Fisher Scientific.

N-[2-[[3-(4-bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89) and 8-Br-cAMP were from R&D Systems (Minneapolis, MN). 2′,5′-dideoxyadenosine (DDA), N,N-dimethyl-(3R,4aR,5S,6aS,10S,10aR,10bS)-5-(acetyloxy)-3-ethenyldodecahydro-10,10b-dihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-1H-naphtho[2,1-b]pyran-6-yl ester β-alanine hydrochloride (NKH477) and 9-(tetrahydro-2-furanyl)-9H-purin-6-amine (SQ22536) were from Merck Biosciences (Middlesex, UK). [2,8-³H]-adenine was from Perkin Elmer (Waltham, MA). 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) was from Molekula (Gillingham, UK). Carbamylcholine chloride (carbachol, CCh) and 3-isobutyl-1-methylxanthine (IBMX) were from Sigma-Aldrich (Gillingham, UK). Ionomycin was from Apollo Scientific (Stockport, UK). Cell culture media, G-418, fluo 4AM and fura 2AM were from Life Technologies (Paisley, UK). 3-[5-(tert-butyl)isoxazol-3-yl]-2-[2-(3-chlorophenyl)hydrazono]-3-oxopropanenitril (ESI-09) was from Biolog Life Science Institute (Bremen, Germany). All PTH analogues were human forms and supplied by either Bachem (Bubendorf, Switzerland) or, for PTHrP(1-36), custom-synthesized by Selleckchem (Boston, MA, USA). Sequences of the analogues used are listed in supplementary material Table S1.

Human orexin-A was from NeoMPS (Strasbourg, France), GF109203X, TCS 1102 and PTx from Tocris Bioscience (Bristol, UK), and CHX, CTx, FDA, forskolin, 3-isobutyl-1-methylxanthine, PI and rotenone from Sigma-Aldrich (St. Louis, MO, USA). Myo-[2-³H]-inositol (PT6-271) and [2,8-³H]-adenine were from PerkinElmer Life and Analytical Sciences (Waltham, MA, USA). Hoechst was from Molecular Probes/Life Technologies (Carlsbad, CA, USA) and Yan 7874 from ChemBridge Co. (San Diego, CA, USA).