E0771 cells were obtained from CH3 Biosystems (Amherst, NY) and cultured in the manufacturer’s recommended media: RPMI 1640 supplemented with 10 mM HEPES, 10% FBS, and antimicrobials (100 U/mL penicillin/100 μg/mL streptomycin/250 μg/mL amphotericin). MC38 cells were obtained from Kerafast (Boston, MA) and cultured in the manufacturer’s recommended media: DMEM containing 25 mM glucose, 2 mM glutamine, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM HEPES, 10% FBS, and antimicrobials (100 U/mL penicillin/100 μg/mL streptomycin/250 μg/mL amphotericin). Neither cell line was authenticated in our laboratory, but all cells were used within 10 passages of obtaining them commercially. Cells were cultured in a 37 °C humidified incubator and split as needed (2–3× weekly). In vitro cell division was measured by incubating 1 × 105 E0771 cells or 5 × 104 MC38 cells in the agents noted for 2 days (or vehicle, the media described above). Forty-eight hours after plating, cells were washed and trypsinized, and live cells were counted by a blinded investigator 48 h later.
Mc38 cells
Manufactured by Kerafast
Sourced in United States
The MC38 cells are a mouse colon adenocarcinoma cell line. They serve as a common model for cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
B16f10 cells, by American Type Culture Collection (2 mentions)
Atcc htb 77, by American Type Culture Collection (1 mentions)
Raw blue cells, by InvivoGen (1 mentions)
Jawsii cells, by American Type Culture Collection (1 mentions)
R8758, by Merck Group (1 mentions)
D2429, by Merck Group (1 mentions)
Mda mb 231 cells, by Thermo Fisher Scientific (1 mentions)
Mccoy 5a, by Thermo Fisher Scientific (1 mentions)
Hct116 cells, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Mda mb 231 cell, by American Type Culture Collection (1 mentions)
Hct116 cell, by American Type Culture Collection (1 mentions)
A375 cells, by American Type Culture Collection (1 mentions)
Ct26 cells, by American Type Culture Collection (1 mentions)
M csf, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hek293 ltv cells, by Cell Biolabs (1 mentions)
17 protocols using mc38 cells
1
Culturing and Measuring E0771 and MC38 Cells
2
Cell Lines for Cancer Research
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The HT29 (ATCC® HTB-38) and CT26 colon (ATCC® CRL-2638™), A549 lung (ATCC® CCL-185™), SKOV-3 ovarian (ATCC® HTB-77™), B16 melanoma (ATCC® CRL-6323™), ZR-75-1 breast (ATCC® CRL-1500™) and U937 leukemic (ATCC® CRL-1593.2™) cell lines used in this study were obtained from the American Type Culture Collection (ATCC®). MC38 cells were acquired from Kerafast (Boston, MA, USA), where they are authenticated, characterised and certified as mycoplasma free. Secondarily, stored cultures are routinely tested for mycoplasma contamination by PCR.
3
Cell Culture Conditions for Diverse Murine Cell Lines
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B16F10 cells were purchased from American Type Culture Collection (ATCC) and cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 100 units/mL aqueous Penicillin G sodium and 100 µg/mL streptomycin (Pen/Strep) at 37 °C in 5% CO2 humidified air. JAWSII cells were purchased from ATCC and cultured in alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM l -glutamine, 1 mM sodium pyruvate, and 5 ng/mL murine GMCSF, 80%; FBS, 20%. MC38 cells were purchased from Kerafast, Inc. and cultured in DMEM containing 10% FBS, 2 mM glutamine, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Hepes), 50 µg/mL gentamycin sulfate, Pen/Strep. TLR2 reporter cell line RAW-Blue cells were purchased from Invivogen and cultured in DMEM containing 10% FBS, 100 mg/mL Normocin, 2 mM glutamine, and 200 µg/mL Zeocin.
4
Culturing Mouse and Human Cancer Cell Lines
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Mouse colon cancer cell line MC38, mouse LLC, and human colon cancer cell line HT-29 were used in the study. MC38 cells were purchased from Kerafast; HT-29 and LLC cells were bought from American Type Culture Collection. Early passage cancer cells were cultured at 37°C and 5% CO2 in either Roswell Park Memorial Institute-1640 media (R8758; Sigma) or DMEM (D2429; Sigma), supplemented with 10% FBS (16000-044; Thermo Fisher Scientific), and 100 IU/mL penicillin-streptomycin (15140-122; Thermo Fisher Scientific).
5
Cell Culture Conditions for Cancer Cell Lines
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All cell lines including human CRC RKO, LoVo, HCT116 cells, human breast cancer MDA-MB-231 cells, melanoma A375 cells were purchased from American Type Culture Collection and mouse CRC MC38 cells were purchased from Kerafast and all mycoplasma free. RKO and LoVo cells were incubated in RPMI-1640 (Gibco) with 10% fetal bovine serum (FBS) (Gibco). HCT116 cells were incubated in McCoy’5A (Gibco) with 10% FBS (Gibco). MDA-MB-231 cells, A375 and MC38 cells were incubated in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) with 10% FBS (Gibco). All cells were cultured at 37°C under 5% CO2.
6
Synthesis and Evaluation of Lipid-Peptide Formulation
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Egg sphingomyelin (eSM) was acquired from Avanti Polar Lipids. Apolipoprotein A-1 mimetic peptide 22A was acquired from Genscript Inc., Piscataway, NJ, USA. Docetaxel (DTX) was acquired from Cayman Chemicals., Ann Arbor, MI, USA. MC-38 cells were purchased from Kerafast, Inc., Boston, MA, USA. Cholesterol-modified CpG1826 was custom ordered from Integrated DNA Technologies, Coralville, IA, USA.
7
Cell Line Preparation and Maintenance
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MC38 cells were purchased from Kerafast. The 4T1, B16F10, and J774A.1 cells were purchased from the American Type Culture Collection. Each cell line was screened for mycoplasma prior to in vitro or in vivo use. All cells were maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin.
8
Murine Macrophage Differentiation and Cell Culture
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Bone marrow cells were flushed from dissected femurs of 8–10‐week‐old C57BL/6 mice and differentiated in RPMI medium containing 10% heat‐inactivated fetal bovine serum, 10 mm HEPES, 2 mM L‐glutamine, 10 mM MEM non‐essential amino acids solution, and penicillin/streptomycin with 20 ng mL−1 M‐CSF (Peprotech) for 5–7 days. Peritoneal macrophages (pMAC) were harvested from mice treated with an intraperitoneal injection of 3% Brewer thioglycollate medium and adherent pMAC were cultured with 20 ng mL−1 M‐CSF. MC38 cells were purchased from Kerafast and CT26 cells were obtained from ATCC. They were cultured in RPMI plus 10% FBS, and penicillin/streptomycin at 37 °C with 5% CO2.
9
Luciferase-Labeled Cell Lines for In Vivo Research
10
Detailed Cell Culture Protocols
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UN-KPC-961 cells were obtained from S. K. Batra at Eppley Institute for Research in Cancer (Omaha, Nebraska). COLO 205 (CCL-222), HCT15 (CCL-225), HT29 (HTB-38), SW480 (CCL-228), HCT116 (CCL-247), HCT8 (CCL-244), NCI-H508 (CCL-253), HepG2 (HB-8065), Hs746T (HTB-135), CT26 (CRL-2638), LS-174T (CL-88), PANC1 (CRL-1469), and NCI-N87 (CRL-5822) cell lines were purchased from the American Type Culture Collection (ATCC) (Baltimore, MD) and maintained in standard conditions following the providers’ instructions. MC38 cells were purchased from Kerafast (Boston, MA). Lvm3b was generated by in vivo selection from the parental cell line LS-174T (ATCC, Baltimore, MD) (15 (link)). UN-KPC-961 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Langley, OK, 11960-044), 7.5% sodium bicarbonate (Gibco, Langley, OK, 25080-094), 1% penicillin-streptomycin (Lonza, 17-745E), 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, F4135), 200 mM l -glutamine (Gibco, Langley, OK, 25030081), 1 mM Hepes (Gibco, Langley, OK, 15630080), and gentamicin (50 mg/ml) (Gibco, Langley, OK, 15750078). MC38 cells were cultured in DMEM, 10% FBS, and 1 mM Hepes.
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