HeLa (5 × 10 3 cells/well) was seeded in a 96-well plate 1 day before transfection at 37C. Subsequently, cells were treated with PEI 25K-CA and PEI 25K at 37°C for 4 hours. At the end of this period, the mixture was replaced with 100 µl of growth medium, and the cells were further incubated for 24 hours. The cell medium was replaced with 100 µl of medium containing 100 µg of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (Bio Basic Inc., Amherst, NY) and incubated for another 4 hours, followed by the addition of 100 µl of DMSO (dimethyl sulfoxide) (Amresco, Solon, OH). The optical density was measured using a microplate reader (SpectraMax M3, Molecular Devices, San Jose, CA) at a wavelength of 570 nm. Cytotoxicity (%) of cells was calculated relative to cells incubated with the growth medium as a control.
3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide
Manufactured by Bio Basic
Sourced in Canada
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide is a chemical compound used in various laboratory applications. It is a yellow tetrazolium salt that can be reduced to form a purple formazan product. This reaction is commonly used as an indicator of cellular metabolic activity and viability.
Automatically generated - may contain errors
Lab products found in correlation
Dmso dimethyl sulfoxide, by Avantor (1 mentions)
Spectramax m3, by Molecular Devices (1 mentions)
Potassium penicillin, by Lonza (1 mentions)
Foetal bovine serum (fbs), by Merck Group (1 mentions)
Visking dialysis tubing, by Serva Electrophoresis (1 mentions)
Dmem f12 medium, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Amphotericin b, by Lonza (1 mentions)
Streptomycin sulfate, by Lonza (1 mentions)
Fungizone, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
α mem medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
4 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide
1
Cytotoxicity Evaluation of PEI Treatments
2
Curcumin Nanoformulation for A431 Cell Line
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Tefose 1500 (T1500) was obtained from Gattefosse (St Priest, France). Span 85 (S85), Span 20 (S20), Tween 80 (T80), and Tween 20 (T20) were purchased from Sigma Chemical Company (St. Louis, Missouri, USA). Curcumin (Cur) purity 95% was purchased from Sigma Aldrich, Germany. Visking Dialysis tubing, diameter 21 mm was purchased from Serva electrophoresis, Germany. All other chemicals used were of analytical grade.
For cell culture, human epidermoid squamous cell carcinoma cell line (A431) was obtained from American tissue culture collection (ATCC) through the bioassay-cell culture laboratory, National Research Centre, Egypt. Cells were suspended in DMEM-F12 medium (purchased from Sigma) with 10% foetal bovine serum (FBS), 1% mixture of 10,000 U/ml potassium penicillin, 10,000 µg/ml streptomycin sulfate, and 25 µg/ml amphotericin B (all were purchased from Lonza, Belgium) and incubated at 37 °C under 5% CO2 using a water jacketed CO2 incubator (Sheldon, TC2323, Cornelius, OR, USA) and regularly checked for absence of any contamination. For MTT assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from BIO BASIC CANADA (Ontario, Canada) and Sodium dodecyl sulfate (SDS) was purchased from ADWIC, Egypt.
For cell culture, human epidermoid squamous cell carcinoma cell line (A431) was obtained from American tissue culture collection (ATCC) through the bioassay-cell culture laboratory, National Research Centre, Egypt. Cells were suspended in DMEM-F12 medium (purchased from Sigma) with 10% foetal bovine serum (FBS), 1% mixture of 10,000 U/ml potassium penicillin, 10,000 µg/ml streptomycin sulfate, and 25 µg/ml amphotericin B (all were purchased from Lonza, Belgium) and incubated at 37 °C under 5% CO2 using a water jacketed CO2 incubator (Sheldon, TC2323, Cornelius, OR, USA) and regularly checked for absence of any contamination. For MTT assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from BIO BASIC CANADA (Ontario, Canada) and Sodium dodecyl sulfate (SDS) was purchased from ADWIC, Egypt.
3
MTT Assay Protocol for Cell Viability
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Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin (PEN) and phosphate buffered saline (PBS) were purchased from HiMedia Laboratories Pvt. Ltd. India. For MTT assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide were purchased from Bio Basic Canada.
4
Abalone Shell-Derived Calcium Carbonate
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The abalone mussel shells used as a source of calcium carbonate (CaCO3) were taken from Bali, Indonesia. The precursors of diammonium hydrogen phosphate ([NH4]2 HPO4), ammonium bicarbonate (NH4HCO3), and ammonium hydroxide (NH4OH) 25% solution were purchased from Merck (USA). HCB was purchased from Sentra Madu Yogyakarta, Indonesia. Fetal bovine serum (FBS) and phosphate buffered saline (PBS) were purchased from Sigma-Aldrich (USA). Penicillin-streptomycin, fungizone, and MEM- α medium were purchased from Gibco (USA), (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) (MTT) was purchased from Biobasic (USA), and dimethyl sulfoxide (DMSO) was purchased from Merck KGaA (Germany).
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