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Sf 900 2 serum

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The Sf-900 II serum is a specialized cell culture medium designed for the growth and maintenance of insect cells. It provides essential nutrients and growth factors to support the optimal proliferation and viability of insect cell lines.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Vero e6 cell, by American Type Culture Collection (1 mentions) Freestyle 293 media, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Sf-900 II serum-free medium (60 citations) SF-900 II serum free media (4 citations) SF-900 II SFM serum-free medium (2 citations)

3 protocols using sf 900 2 serum

1

Silkworm Larvae Cultivation and BmN Cell Culture

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Silkworm larvae were raised on fresh mulberry leaves at 27 °C in 75–80% humidity in an artificial climate incubator. The BmN cell line was cultured in Sf-900 II serum-free medium (Gibco BRL) with 10% (v/v) fetal bovine serum (Gibco BRL) at 27 °C. The cells preserved in our lab were derived from the silkworm ovary.
Si H., Cao Y., Zhu H., Li D., Lv Z., Sheng Q, & Nie Z. (2017). Transposable Element Bm1645 is a Source of BmAGO2-associated Small RNAs that affect its expression in Bombyx mori. BMC Genomics, 18, 201.
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2

Culturing VERO E6 and Sf9 Cells

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VERO E6 cells (ATCC CRL-1587) were grown at 35 °C in Eagle’s Minimum Essential Medium (EMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Life Technologies, Grand Island, NY, USA). Spodoptera frugiperda (Sf9) cells were grown at 27 °C in Sf-900™ II serum free media supplemented with antibiotics (penicillin, 10,000 U/mL; streptomycin, 10,000/mL) (GIBCO, Life Technologies, Grand Island, NY, USA).
Zhang Y., Brooks W.A., Goswami D., Rahman M., Luby S.P, & Erdman D.D. (2014). A duplex recombinant viral nucleoprotein microbead immunoassay for simultaneous detection of seroresponses to human respiratory syncytial virus and metapneumovirus infections. Journal of virological methods, 206, 55-62.
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3

Culturing Sf9 and HEK293S Cells

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Spodoptera frugiperda Sf9 cells in suspension were passaged in Sf-900 II serum-free media (Gibco) with 5% fetal bovine serum (FBS) at 27 °C in an orbital shaker. HEK293S GnTI- cells in suspension were passaged in Freestyle 293 media (Gibco) supplemented with 2% FBS at 37 °C, 8% CO2, and 80% humidity in an orbital shaker.
Pietz H.L., Abbas A., Johnson Z.L., Oldham M.L., Suga H, & Chen J. (2023). A macrocyclic peptide inhibitor traps MRP1 in a catalytically incompetent conformation. Proceedings of the National Academy of Sciences of the United States of America, 120(11), e2220012120.
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