Anti lc3 2 1 12741

Briefly, rabbit anti-Bax polyclonal antibody (#2772) and rabbit anti-p65 (#8242), anti-p-p65 (#3033), anti-IκBα (#4812), anti-GFP (#2956), anti-Beclin-1 (#3495), and anti-LC3 II/I (#12741) monoclonal antibodies were all obtained from Cell Signalling Technology (Danvers, MA, USA). Rabbit anti-Bcl-2 (ab196495), anti-Histone H3 (ab1791) and anti-β-actin (ab8227) polyclonal antibodies and horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (ab205718) were obtained from Abcam (Cambridge, UK). RIPA buffer and Halt™ Protease and Phosphatase Inhibitor Cocktail were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Enhanced chemiluminescence (ECL) reagent and JC-1 were obtained from Millipore (Bedford, MA, USA). Trypsin, PDTC and bromodeoxyuridine (BrdU) were obtained from Sigma (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), foetal bovine serum (FBS), and penicillin-streptomycin solution were obtained from Gibco (Grand Island, NY, USA). Collagenase II was obtained from Worthington (Minnesota, USA). H₂O₂ was obtained from Sangon (Shanghai, China).

Diltiazem (DIL) was purchased from Shanghai Xinyi Wanxiang Pharmaceutical Co., Ltd. (Shanghai, China). Cyanidin-3-glucoside (C3G, #C832095, with the purity ≥98%) was obtained from Macklin (Shanghai, China). The anti-USP19 (ab167059), anti-Beclin1 (ab210498), anti-Transferrin receptor 1 (TfR1, ab1086), anti-NCOA4 (ab86707), and anti-Ubiquitin (linkage-specific K11) antibodies were provided by Abcam (Cambridge, UK). Anti-ferritin heavy chain (FTH1, #3998) and anti-LC3I/II (#12741) antibodies were supplied from Cell Signaling Technology (Boston, USA). Anti-GAPDH antibody, Goat Anti-Mouse IgG (H + L) HRP second antibody, and Goat Anti-Rabbit IgG (H + L) HRP second antibody were produced by Affinity (Cincinnati, USA).