All the isolates and dexamethasone were dissolved in DMSO (less than 0.1% in the cell-based assays). Fetal bovine serum (FBS) was purchased from Cytiva (Marlborough, MA, USA). Phosphate-buffered saline (PBS) powder was purchased from Beijing Langeco Technology Co., Ltd. (Shunyi District, Beijing, China). RPMI 1640 medium, penicillin-streptomycin (P/S), and pancreatin were purchased from Life Technologies (Grand Island, NY, USA). All the chemicals (analytical grade) were obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Pudong Zhangjiang High Tech. Park, Shanghai, China), except those otherwise specified. The TNF-α, IL-1β, IL-8, and IL-10 ELISA kits were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Minhang District, Shanghai, China).
Tnf α
Manufactured by Shanghai Enzyme-linked
Sourced in China
TNF-α is a laboratory reagent used in enzyme-linked immunosorbent assays (ELISAs) to detect and quantify the presence of the tumor necrosis factor-alpha (TNF-α) cytokine in biological samples. TNF-α is a pro-inflammatory cytokine that plays a key role in immune response and inflammation.
Automatically generated - may contain errors
Lab products found in correlation
Il 1β, by Shanghai Enzyme-linked (99 mentions)
Interleukin 6 (il 6), by Shanghai Enzyme-linked (72 mentions)
Il 10, by Shanghai Enzyme-linked (63 mentions)
Tgf β, by Shanghai Enzyme-linked (36 mentions)
Elisa kit, by Shanghai Enzyme-linked (27 mentions)
Il 18, by Shanghai Enzyme-linked (18 mentions)
Il 17, by Shanghai Enzyme-linked (18 mentions)
Ifn γ, by Shanghai Enzyme-linked (9 mentions)
Silica gel, by Qingdao Haiyang Chemical (9 mentions)
Ros kit, by Shanghai Enzyme-linked (9 mentions)
Pancreatin, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Cytiva (3 mentions)
Ast and alt assay kits, by Nanjing Jiancheng (3 mentions)
P iκbα, by Affinity Biosciences (3 mentions)
Nlrp3, by Affinity Biosciences (3 mentions)
Histone h3, by Affinity Biosciences (3 mentions)
Gapdh, by Affinity Biosciences (3 mentions)
Erythromycin, by Meilun (3 mentions)
18 protocols using tnf α
1
Compound Cytokine Regulation in Cells
2
Acne Treatment Protocol with TCM Herbs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The IDDS prescription (rhubarb and sulfur and Fusidic Acid Cream were obtained from the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine) (rhubarb:20210910, sulfur:20211009, Fusidic Acid Cream:C59765).
The activity detection kit (SOD, CAT, SDH and NADP-MDH) were purchased from Beijing Solarbio Science & Technology Co., Ltd. and Elisa-kit (IL-6, IL-10 and TNF-α) were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd.
C. acnes (ATCC11827) samples were purchased from Shanghai Zhichenhui Biotechnology Company Limited (Shanghai, China).
The study utilized Wistar rats, weighing between 180 and 200 g, procured from SPF (Beijing) Biotechnology Co., Ltd in Beijing, China.
The activity detection kit (SOD, CAT, SDH and NADP-MDH) were purchased from Beijing Solarbio Science & Technology Co., Ltd. and Elisa-kit (IL-6, IL-10 and TNF-α) were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd.
C. acnes (ATCC11827) samples were purchased from Shanghai Zhichenhui Biotechnology Company Limited (Shanghai, China).
The study utilized Wistar rats, weighing between 180 and 200 g, procured from SPF (Beijing) Biotechnology Co., Ltd in Beijing, China.
3
Paederia scandens Hepatoprotective Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
Paederia scandens (Lour) Merr. (place of origin: Hubei, batch no. 181201) was purchased from Hainan Linshishengtai Pharmaceutical Co. Ltd. Acetaminophen tablets (batch no. 190402) were acquired from Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd. Silybin capsules (batch no. 850703059) were purchased from Tianjin Tasly Sants Pharmaceutical Co., Ltd. AST and ALT assay kits were obtained from Nanjing Jiancheng Bioengineering Institute. Rat ELISA kits of iNOS (LOT202005), IL-10 (LOT202006), IL-6 (LOT202006), TNF-α (LOT202006), and NF-κB (LOT202006) were obtained from Shanghai Enzyme-Linked Biotechnology Co., Ltd. Male Sprague Dawley rats were purchased from Changsha Tianqin Biological Technology Co., Ltd. Our study was performed according to the international, national, and institutional guidelines for animal experiments. The animal protocol was approved by the Ethics Review Committee for Animal Experimentation of Hainan Medical University. The ethical inspection number is HYLL-2021-387.
+ Expand
Paederia scandens (Lour) Merr. (place of origin: Hubei, batch no. 181201) was purchased from Hainan Linshishengtai Pharmaceutical Co. Ltd. Acetaminophen tablets (batch no. 190402) were acquired from Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd. Silybin capsules (batch no. 850703059) were purchased from Tianjin Tasly Sants Pharmaceutical Co., Ltd. AST and ALT assay kits were obtained from Nanjing Jiancheng Bioengineering Institute. Rat ELISA kits of iNOS (LOT202005), IL-10 (LOT202006), IL-6 (LOT202006), TNF-α (LOT202006), and NF-κB (LOT202006) were obtained from Shanghai Enzyme-Linked Biotechnology Co., Ltd. Male Sprague Dawley rats were purchased from Changsha Tianqin Biological Technology Co., Ltd. Our study was performed according to the international, national, and institutional guidelines for animal experiments. The animal protocol was approved by the Ethics Review Committee for Animal Experimentation of Hainan Medical University. The ethical inspection number is HYLL-2021-387.
4
Biochemical Assays and Reagents Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
COP (purity >98%) was purchased from Shanghai Yuan Ye Biotechnology Co., Ltd (Shanghai, China). Dextra sulfate sodium was obtained from MP Biomedical (Irvine, CA, USA). Cefadroxil (purity >99%), terramycin (purity >98%) and erythromycin (purity >99%) were purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Mesalazine (MSZ) was obtained from Losan Pharma GmbH, Germany. ELISA kits (TNF-α, IL-6, IL-10, IL-18, IL-1β, TGF-β, and IFN-γ) were obtained from Shanghai Enzyme-linked Biotechnology Co., Ltd (Shanghai, China). BCA kit and myeloperoxidase (MPO) assay kit were obtained from the Jiancheng Bioengineering Institute of Nanjing (Nanjing, Jiangsu, China). Primary antibodies against p65, IκBα, p-IκBα, NLRP3, ASC, Capase-1, Caspase-1 p10, GAPDH and Histone H3 were purchased from Affinity Biosciences (OH, USA). All other reagents and chemicals were at least of analytical grade.
5
Evaluation of Jat and Mesalazine in Colitis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DSS (36–50 kDa) was purchased from MP Biomedicals (Canada). Phospho–NF–κB p65 Rabbit mAb (cat. no. 3033) and NF-κB p65 Rabbit mAb (cat. no. 8242) were obtained from Cell Signaling Technology, Inc. TLR4 (19811-1-AP), zonula occludens-1 (ZO-1) (21773-1-AP), Occludin (27260-1-AP), and GAPDH (10494-1-AP) were obtained from Proteintech (USA). MyD88 (AF5195) was purchased from Affinity Biosciences (USA). COX-2 (bs-0732R) was obtained from BIOSS. Jat Standard (purity ≥98%, Cat. No. 6681-15-8) was purchased from Chroma Biotechnology Co. Ltd. (Chengdu, China). Mesalazine (5-ASA) was purchased from Losan Pharma GmbH (lot. no. L20235A). Jat and 5-ASA were dissolved in water to make an oral suspension. IL-1β, TNF-α, MPO, IL-10, and TGF-β enzyme-linked immunosorbent assay (ELISA) kits were purchased from Shanghai Enzyme-Linked Biotechnology (Shanghai, China) Co., Ltd.
6
Serum Cytokine Profiling in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
7
Herbal Compound Bioactivity Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The T. ruticarpum (A.Juss.) T.G.Hartley, Z. officinale Roscoe, P. ginseng C.A.Mey, and Z. jujuba Mill were purchased from Lvye Pharmaceutical Co., Ltd. (Beijing, China) (Cat: 21030801, 22041904, 21112603, 21081801) and the plants were identified and authenticated through microscopic identification and thin layer chromatography (TLC) by company according to the Chinese Pharmacopoeia (2020). The enzyme-linked immunosorbent assay kits of IL-6, IL-10, and TNF-α were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Cat: ml064292; ml002813; ml002859). The primary antibodies of albumin (ALB) (1: 1,000), and Heme Oxygenase 1 (HMOX1) (1: 1,000) were obtained from Wuhan Sanying Biotechnology Co., Ltd. (Cat: 16475-1-AP; 10701-1-AP).
8
Sodium Butyrate Modulates Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium butyrate (Figure 1(a) ; HPLC ≥ 98.5% purity) was obtained from Sigma-Aldrich (Shanghai) Trading Co. Ltd. (China). TNF-α, IL-1β, IL-6, IL-10, TGF-β, and IL-17 assay kits were purchased from Shanghai Enzyme-linked Biotechnology Co. Ltd. (Shanghai, China). Primary antibodies, including anti-iNOS (Cell Signaling, 13120S), anti-Arg-1 (Cell Signaling, 93668S), anti-ROR gamma(t) (Invitrogen, 14-6988-82), anti-FoxP3 (Beijing Biosynthesis Biotechnology Co. Ltd., Beijing, China, bs-10211R), anti-β-actin (Proteintech, 20536-1-AP), and anti-insulin (Abcam, ab181547). Goat anti-rabbit secondary antibodies (cat. no. 10285-1-AP) were obtained from ProteinTech Group, Inc. (Chicago, IL, USA).
9
Quantifying Inflammatory Cytokines in Ileal Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 0.1 g sample of middle ileum tissue was collected and homogenized with ten times the volume of pre-cooled PBS. The supernatant was then subjected to thorough homogenization and the protein content was subsequently measured. The concentrations of TNF-α (ml002790, Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China), IL-4 (ml059838, Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China), and IL-10 (ml059830, Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China) in the ileal tissue homogenate and serum were determined using enzyme-linked immunoassay kits. The determination procedures were conducted following the instructions provided by the manufacturer.
10
Quantifying Inflammatory Cytokines with ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ELISA kit detected the concentration of IL-1β, IL-6, MPO and TNF-α (Shanghai Enzyme-linked Biotechnology Co., Ltd., China). According to the instruction of ELISA kit, the standard (50 μL) and sample (40 μL Sample diluent and 10 μL sample) were added to the standard holes and sample holes of microtiter plate, respectively. The culture plates were incubated at 37°C in 5% CO2 for 30 min and then rinsed with washing solution. Then 50 μL enzyme-labeled reagent was added into each well, and the samples were incubated for 30 min and washed. Then 50 μL color developer A and color developer B were added, mixed, and stained in the dark for 10 min. After the reaction was stopped by adding stop solution, the absorbance density (OD) was measured at 450 nm by enzyme-labeled instrument (Molecular, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!