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Cck 8

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The CCK-8 (Cell Counting Kit-8) is a colorimetric assay used to measure cell viability and cytotoxicity. It utilizes a water-soluble tetrazolium salt, which is reduced by cellular dehydrogenases, producing a colored formazan dye that can be detected and quantified using a spectrophotometer.

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Lab products found in correlation

Microplate reader, by Thermo Fisher Scientific (16 mentions) Microplate reader, by Agilent Technologies (4 mentions) Synergy h1, by Agilent Technologies (4 mentions) Dichloromethane, by Tianjin Damao (3 mentions) Isopropanol, by Tianjin Damao (3 mentions) Ethyl alcohol, by Tianjin Damao (3 mentions) Cck 8, by Agilent Technologies (2 mentions) Elx808, by Agilent Technologies (2 mentions) Cmax plus, by Molecular Devices (2 mentions) E607309, by Sangon (2 mentions) Multiskan go, by Thermo Fisher Scientific (2 mentions) Cck 8, by Thermo Fisher Scientific (2 mentions) Multiskan fc, by Thermo Fisher Scientific (2 mentions) Cck 8 solution, by Sangon (1 mentions) Lenvatinib, by MedChemExpress (1 mentions) Cabozantinib, by MedChemExpress (1 mentions) Cell counting kit 8 solution, by Dojindo Laboratories (1 mentions) Gefitinib, by MedChemExpress (1 mentions) Automatic microplate reader, by Thermo Fisher Scientific (1 mentions) Mir 152 3p mimic, by RiboBio (1 mentions)

103 protocols using cck 8

1

Cell Proliferation Assay Protocol

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Cells were seeded in 6-well plates at a density of 400 cells/well and cultured for 10 days. After xation with 4 % paraformaldehyde for 15 minutes, sections were dyed with 0.1% crystal violet for 15 minutes. Only colonies that contained more than 50 cells were counted.
Cell Counting Kit-8 (CCK8)
Cell proliferation was assessed using CCK8 (MedChemExpress, China) according to the manufacturer's instruction. In brief, 10 µl of CCK8 solution was added to each well and the plates were incubated for an hour at 37°C. Optical density was acquired at 450 nm from a Bio-Rad microplate reader (Model 680, Bio-Rad, USA).
Yang P., Li C., Zhou Q., Zhang X., Kou Y., Feng Q., Wang H., Su R., Liu H., Hasegawa T., & Li M. (2021). Notum Promotes Proliferation and Migration of Oral Squamous Cell Carcinoma Via Shh/p-GSK3β/β-Catenin Pathway.
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2

Cell Viability Assessment by CCK-8 Assay

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Cell viability was examined by CCK-8 (MedChemExpress, China) assay in our study. Briefly, the neurons (approximately 5000 cells/well) were seeded in 96-well plate and subjected to various treatments described above. BPH-1 and WPMY-1 cells were then cultured in the cell incubator for 0, 24, 48 or 72 h, respectively. At different time points, 10 μl CCK-8 solution (Sangon Biotech, Shanghai, China) was added to each well, and cells were incubated in the dark for 1 h. The absorbance at 450 nm was measured by a microplate reader (Thermo Labsystems, Vantaa, Finland).
Fu X., Liu J., Liu D., Zhou Y., Guo Y., Wang Z., Yang S., He W., Chen P., Wang X., DiSanto M.E, & Zhang X. (2022). Glucose-regulated protein 78 modulates cell growth, epithelial–mesenchymal transition, and oxidative stress in the hyperplastic prostate. Cell Death & Disease, 13(1), 78.
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3

Cell Proliferation and Colony Formation Assay

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For cell proliferation assay, ccRCC cells were seeded in 96‐well plates (1,500 cells/well) in fresh medium. At each time point, 10 μL of Cell Counting Kit‐8 solution (CCK‐8; Dojindo, Tokyo, Japan) was added directly to each well, followed by incubation for 3 h at 37°C. For drug treatment, cells were seeded in 96‐well plates (1,500 cells/well) with 100 μL culture medium, then treated with 2 μmol/L gefitinib (MedChemExpress, Princeton, NJ, USA), lenvatinib (MedChemExpress) or cabozantinib (MedChemExpress) for 72 h, followed by adding CCK‐8 solution. The optical density was measured at 450 nm (OD450) by Microplate Reader (BioTek, Vermont, USA). Each experiment was performed in triplicate.
In the colony formation assay, cells were plated in 6‐well plates (500 cells/well) in 2 mL of fresh medium and cultured for 10 days. Visible colonies were stained with 0.5% crystal violet, and colonies were counted with ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Luo L., Wei D., Pan Y., Wang Q., Feng J., Yu B., Kang T., Luo J., Yang J, & Gao S. (2023). MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria‐dependent dephosphorylation of EGFR. Cancer Communications, 43(7), 808-833.
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4

Probiotic Growth Kinetics in CCK-8 Assay

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BC@CTM and BC
were taken in NB broth medium and diluted to OD600 = 0.5. 190 μL
of each probiotic and 10 μL of CCK-8 (MedChemExpress) were added
to a 96-well plate and incubated in an incubator at 37°C. The
OD450 values of probiotics were then recorded hourly using a microplate
spectrophotometer.
Yang J., Peng M., Tan S., Ge S., Xie L., Zhou T., Liu W., Zhang K., Zhang Z., Liu J, & Shi J. (2023). Calcium Tungstate Microgel Enhances the Delivery and Colonization of Probiotics during Colitis via Intestinal Ecological Niche Occupancy. ACS Central Science, 9(7), 1327-1341.
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5

Investigating miR-152-3p Regulation of MTEC1 Cell Viability

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The MTEC1 cells were inoculated in a 96-well plate at a density of 5 × 103 cells/well and cultured at 37 °C in an incubator containing 5% CO2 for 12 h. Then, 50 nM miR-152-3p mimic, 100 nM miR-152-3p inhibitor, or mimic/inhibitor negative control (mimic/inhibitor-nc) (RiboBio, Guangzhou, China) were transfected into the MTEC1 cells using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions. After 48 h of transfection, 10 µL CCK-8 (MedChemExpress, Monmouth, NJ, USA) was added to each well and reacted in an incubator containing 5% CO2 at 37 °C for 2 h. Subsequently, the absorbance of the MTEC1 cells plated in a 96-well plate was measured at OD450 nm using an automatic microplate reader (Thermo Fisher Scientific), and the influence of miR-152-3p expression on the viability of MTEC1 cells was analyzed.
Li Y., Wang X., Wu Q., Liu F., Yang L., Gong B., Zhang K., Ma Y, & Li Y. (2022). miR-152-3p Represses the Proliferation of the Thymic Epithelial Cells by Targeting Smad2. Genes, 13(4), 576.
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6

Cisplatin Cytotoxicity Assay in EC Cells

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For cell viability analysis, EC cells were seeded into 96-well plates at a density of 1×104 to 1.5×104 cells/well, followed by incubation for 24 h. The attached cells were exposed to cisplatin at various concentrations for 48 h. The dose of cisplatin was 0, 0.125, 0.25, 0.5, 1, 2, and 4 μg/ml in RL95-2 cells, while it was 0, 0.25, 0.5, 1, 2, 4, 8, and 16 μg/ml in ARK-2 cells. The number of viable cells was determined using the cell counting kit-8 (CCK-8, MedChem Express, USA) assay following the manufacturer’s instructions. The experiment at each concentration was carried out in triplicate.
Xie W., Liu N., Wang X., Wei L., Xie W, & Sheng X. (2021). Wilms’ Tumor 1-Associated Protein Contributes to Chemo-Resistance to Cisplatin Through the Wnt/β-Catenin Pathway in Endometrial Cancer. Frontiers in Oncology, 11, 598344.
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7

Cytotoxicity Evaluation of PGF2α, Ebopiprant, and U0126

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Different concentrations of PGF2α, Ebopiprant, and U0126 were added to 96-well plates, and the drug and medium were replaced every 2–3 days. On the 10th day, a cell counting kit (CCK)-8 (MedChemExpress, Monmouth Junction, NJ, USA) was used to detect the absorbance at 450 nm using a microplate reader, according to the manufacturer’s instructions, to obtain the safe working concentration of each drug.
Zhu R., Wang X.H., Wang B.W., Ouyang X., You Y.Y., Xie H.T., Zhang M.C, & Jiang F.G. (2023). Prostaglandin F2α Regulates Adipogenesis by Modulating Extracellular Signal-Regulated Kinase Signaling in Graves’ Ophthalmopathy. International Journal of Molecular Sciences, 24(8), 7012.
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8

Evaluating MCM Viability with PA

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MCMs (5 × 104 per mL) were seeded into 96-well plates, allowed to attach, and then treated separately with various concentrations of PA (0, 150, 350, 450, and 550 µM) for 24 h in a 5% CO2 incubator at 37°C. The viability of MCMs was determined by CCK-8 (MedChemExpress Co., China). CCK-8 solution (10 µL per well) was added to each well. The cells were further incubated at 37°C for 2 h, the cell viability of each treatment was quantified by determining absorbance in each well at 450 nm using a microplate reader (Thermo Fisher Scientific Co., USA). Cell viability = (Treated Group OD−Blank Group OD)/(Control Group OD−Blank Group OD).
Zhang X., Mao M, & Zuo Z. (2022). Palmitate Induces Mitochondrial Energy Metabolism Disorder and Cellular Damage via the PPAR Signaling Pathway in Diabetic Cardiomyopathy. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 15, 2287-2299.
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9

Proliferation Assay of SCAP

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The proliferation ability of SCAP was examined using CCK-8 (MedChemExpress, Monmouth Junction, NJ, United States). Cells were seeded in 96-well plates at a density of 3 × 103 cells/well. The cells were treated with CCK-8 reagent on days 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 and incubated at 37°C for 2 h. Then, the absorbance was measured at 450 nm using a microplate reader.
Liu J., Wang X., Song M., Du J., Yu J., Zheng W., Zhang C, & Wang Y. (2020). MiR-497-5p Regulates Osteo/Odontogenic Differentiation of Stem Cells From Apical Papilla via the Smad Signaling Pathway by Targeting Smurf2. Frontiers in Genetics, 11, 582366.
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10

Cell Viability Assay with CCK-8

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Cells were seeded into a 96-well plate (2 × 103 cells/well), and cultivated for indicated times. 10 μL cell counting Kit-8 (CCK-8) (MedchemExpress, USA) was added into each well. Thereafter, the cells continued to cultivate for 2 h. The absorbance at 450 nm was read with Microplate Reader (Thermo Scientific, USA).
Wang H., Wei X., Liu L., Zhang J, & Li H. (2024). Suppression of A-to-I RNA-editing enzyme ADAR1 sensitizes hepatocellular carcinoma cells to oxidative stress through regulating Keap1/Nrf2 pathway. Experimental Hematology & Oncology, 13, 30.
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