RAW264.7 cells (Murine macrophages) were cultured using Dulbecco’s modified Eagle medium (DMEM) from Welgene (Seoul, Republic of Korea), supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, and 100 unit/mL of antibiotics from Gibco BRL (Rockville, MD). The cells were incubated at 37 °C in a humidified atmosphere containing 5% (v/v) air/CO2. For the cell viability assay, RAW264.7 cells (5 × 103/well) were seeded into a 96-well cell culture plate and allowed to attach and stabilize for 18 h. After incubation, the growth medium was replaced with a fresh medium alone. The synthetic compounds were then introduced to the cells at a concentration of 20 µg mL−1, and the cells were further incubated for 24 h. Subsequently, the sample-treated culture medium was removed and 100 µg mL−1 of 3-(4,5-dimetnythiazol-2-yl)-2,5-diphenyl-thetazolium bromide (MTT) was added to each well. After a one-hour incubation, the purple formazan generated as a result of cellular respiration was dissolved in a 200 µL solution of diemthyl sulfoxide (DMSO), and the absorbance at 560 nm was measured using a multi-plate reader. The analyses were repeated three times, and the results were expressed as the means of three independent experiments.
Dulbecco s modified eagle medium dmem
Manufactured by Welgene
Sourced in United States
Dulbecco's Modified Eagle Medium (DMEM) is a widely used cell culture medium formulated to support the growth and maintenance of various cell types. It provides essential nutrients, vitamins, and inorganic salts required for cell proliferation and survival. DMEM is a versatile medium suitable for a broad range of cell lines and applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (12 mentions)
Fetal bovine serum (fbs), by Welgene (8 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (8 mentions)
Penicillin, by Welgene (5 mentions)
Rpmi 1640, by Welgene (5 mentions)
Phosphate buffered saline (pbs), by Welgene (5 mentions)
β actin, by Cell Signaling Technology (4 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
Fetal bovine serum (fbs), by Merck Group (3 mentions)
Penicillin streptomycin, by Welgene (3 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (3 mentions)
β actin, by Merck Group (3 mentions)
Dithiothreitol (dtt), by Merck Group (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Trizol, by Thermo Fisher Scientific (3 mentions)
Hct116, by Welgene (2 mentions)
Protein assay reagent kit, by Bio-Rad (2 mentions)
Penicillin streptomycin solution, by Welgene (2 mentions)
Dulbecco s phosphate buffered saline dpbs, by Welgene (2 mentions)
Type 1 collagen, by BD (2 mentions)
45 protocols using dulbecco s modified eagle medium dmem
1
MTT Assay for RAW264.7 Cell Viability
2
Culturing Human Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human malignant astroglioma cell line U251-MG was obtained from Dr Benveniste EN (University of Alabama at Birmingham, Birmingham, AL, USA). Human colorectal carcinoma cell line HCT116 and human malignant melanoma cell line A375 were obtained from Korean Cell Line Bank (Seoul, Korea). U251-MG were cultured in Minimum Essential Medium (MEM) (Welgene, Daegu, Korea), HCT116 in McCoy’s 5A Medium (McCoy’s) (Welgene), and A375 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Welgene). Media were supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco, Logan, Utah, USA), 1x antibiotic (Welgene).
3
Cytokine-deprived cell culture protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dulbecco's modified eagle medium (DMEM) and the amino acid-deprived DMEM formulations were purchased from Welgene (Daegu, Korea). Fetal bovine serum (FBS), mitomycin C, recombinant human epithelial growth factor and insulin solution were obtained from Sigma-Aldrich (St.Louis, MO). Dialyzed FBS (dFBS), HEPES, and anti-FAK (pY397) antibodies were purchased from Life Technologies (Grand Island, NY). The anti-FAK antibody was obtained from Millipore (Billerica, MA). Penicillin-Streptomycin (pen/strep) solution was purchased from Mediatech, Inc. (Manassas, VA). Matrigel was obtained from Corning (Corning, NY) and protein assay reagent kits were obtained from Bio-Rad Laboratories (Hercules, CA). A10021B and Modified Diets were obtained from Central Lab. Animal, Inc. (Seoul, Korea; details in Supplementary Table S1 ).
4
Evaluating Cell Proliferation on Gelatin Nanofiber Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fibroblasts (NIH-3 T3 cells) were used to evaluate cell proliferation on e-beam-irradiated gelatin nanofiber scaffolds. Samples were cut into a disk shape (22 mm in diameter), immersed in 70% ethanol for sterilization for 24 h, and then rinsed with PBS for 24 h before seeding. Prepared gelatin fibrous sheets were placed in 12-well culture plates with glass rings to prevent floating. Cells were seeded at a density of 5.0 × 104 cells/disk in 1 mL Dulbecco’s modified Eagle medium (DMEM; Welgene, Korea) containing 10% fetal bovine serum (FBS; Welgene, Korea) and 1% antibiotics (Penicillin-Streptomycin solution; Welgene). Seeded cells were cultured for 1, 3, 5, or 7 days under standard cell culture conditions. After the designated culture period, the culture medium was replaced with 1 mL of medium containing 10% WST-1 reagent. Subsequently, samples were incubated under standard cell culture conditions for 2 h, and 100 μL of the incubated solution was then transferred to a 96-well culture plate. A microplate reader (PHOmo; Autobio Labtec Instruments Co., Ltd., China) was used to measure the absorbance of formazan at 450 nm.
5
Hep3B Cell Culture for PGRMC1 Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All cell culture reagents were purchased from Welgene (Gyeongsan, Korea). Hep3B cells were obtained from Korean Cell Line Bank (KCLB, 88064). Hep3B cells and liver primary cells were maintained at 37 °C in a 5% CO2 atmosphere in Dulbecco’s Modified Eagle Medium (DMEM) (Welgene) supplemented with 5% (vol/vol) foetal bovine serum, penicillin (100 U/mol) and streptomycin (100 μg/ml). Cells were washed once with Dulbecco’s Phosphate-Buffered Saline (DPBS) (Welgene) and incubated with low glucose medium (50 mg/dl, w/o FBS; Welgene) for 18 h. P4 was treated with CD-FBS for steroid hormone delivery. All cell experiments were repeated at least three times.
For PGRMC1 overexpression, Hep3B cells were transfected with 2.5 µg of human PGRMC1 expression plasmid and Lipofectamine 2000 (Invitrogen) in Opti-MEM (Gibco) medium according to the manufacturer’s protocol. For PGRMC1 knockdown, Hep3B cells were transfected with control siRNA or PGRMC1 siRNA #1 (5′-CAGUACAGUCGCUAGUCAA-3′) and #2 (5′-CAGUUCACUUUCAAGUAUCA-U-3′) purchased from Bioneer (Daejeon, Korea).
For PGRMC1 overexpression, Hep3B cells were transfected with 2.5 µg of human PGRMC1 expression plasmid and Lipofectamine 2000 (Invitrogen) in Opti-MEM (Gibco) medium according to the manufacturer’s protocol. For PGRMC1 knockdown, Hep3B cells were transfected with control siRNA or PGRMC1 siRNA #1 (5′-CAGUACAGUCGCUAGUCAA-3′) and #2 (5′-CAGUUCACUUUCAAGUAUCA-U-3′) purchased from Bioneer (Daejeon, Korea).
6
Antioxidant and Anti-inflammatory Effects of Chaenomeles sinensis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HaCaT and RAW264.7 cells were purchased from the American Type Cell Collection (ATCC, Manassas, VA, USA). Testosterone propionate was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol extract of Chaenomeles sinensis was provided by Sunchang Institute of Health and Longevity (Sunchang, Korea). Dulbecco’s modified Eagle medium (DMEM), heat-inactivated FBS, PBS, and penicillin/streptomycin (Pen/Strep) were purchased from Welgene (Daegu, Korea). Polyclonal antibody against NF-E2-related factor 2 (NRF2) was purchased from Cell Signaling Technology (Danvers, MA, USA). Monoclonal antibodies against 8-hydroxydeoxyguanosine (8-OHdG) and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyclonal antibody against 4-hydroxynonenal (4-HNE) was purchased from Abcam (Cambridge, MA, USA). 2,2’-diphenylpicrylhydrazyl (DPPH) was purchased from Cayman (Ann Arbor, MI, USA) and 2,2’-azinobis diammonium salt (ABTS) was purchased from Roche Diagnostics (Mannheim, Germany). Monoclonal antibodies against iNOS and COX-2 were purchased from BD Transduction Laboratories (Franklin Lakes, NJ, USA).
7
Cell Culture and Conditioned Medium Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BM-MSCs, adipose tissue-derived MSCs (AT-MSCs) and T-MSCs were cultured in low-glucose Dulbecco's modified Eagle medium (DMEM; Welgene) in 100-mm cell culture plates. The BM-MSCs were purchased from the Severance Hospital Cell Therapy Center and the AT-MSCs were purchased from the American Type Culture Collection (ATCC). In the present study the T-MSCs were obtained previously (IRB File no. EUMC 2018-01-011-002) and maintained from the same patients as previously described (23 (link)). To prepare for cell injection, cells were collected at 80% confluence with trypsin treatment followed by 3 washes with PBS. Prior to injection with BM cells (BMCs) into the mice, MSCs were resuspended with low-glucose DMEM at a final volume of 200 µl for tail vein injection. To generate conditioned medium (CM) to analyze cellular soluble factors, cells at 80% confluence were washed four times with PBS, and the medium was replaced with serum-free DMEM. The medium was collected after 48 h of culture as previously described (19 (link)), centrifuged at 190 × g for 5 min at 25°C, passed through a 0.2-µm filter (Merck Millipore), and concentrated 20-fold by high-speed centrifugal filtration (Sorvall LYNX4000, Thermo Fisher Scientific, Inc.). The concentrated CM was then frozen and stored at -80°C for future use. As a negative control, serum-free culture medium was processed using the same method.
8
Amylosucrase-Catalyzed Synthesis of Sucrose Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sucrose, resveratrol, and β-piceid were purchased from Sigma-Aldrich (USA). Water, methanol, and acetonitrile (high-performance liquid chromatography grade) were purchased from Burdick & Jackson (USA) for purification of transfer products. Dulbecco’s modified Eagle medium (DMEM) was purchased from WelGENE Inc. (Korea). Fetal bovine serum (FBS) and antibiotics (penicillin/streptomycin) were purchased from Gibco (Thermo Fisher Scientific, USA). Diphenyl-2-picrylhydrazyl (DPPH) and 2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) were purchased from Sigma-Aldrich. All other chemicals were of reagent grade and purchased from Sigma-Aldrich. The recombinant amylosucrase from D. geothermalis (DGAS) was prepared in E. coli as previously described [16 (link)].
9
PLGA-Hydroxyapatite Scaffold Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Poly(D,L-lactic-co-glycolic acid) (PLGA) was purchased from Lakeshore Biometerials (Essen, Germany) and Hydroxyapatite (HA) was form Sigma-Aldrich (St. Louis, MO) were used for scaffold fabrication. The molar ratio of lactide to glycolide was 50/50 and their molecular mass averages of the weight (Mw) and number (Mn) are 69 kDa and 42 kDa, respectively. MG-63 osteoblast-like cell for this study was obtained from KCLB (Seoul, Korea) Dulbecco’s Modified Eagle Medium (DMEM) was obtained from Welgene (Daegu, Korea), fetal bovine serum (FBS) was purchased from Gibco (Thermo Fisher Scientific, Waltham, USA)and penicillin/streptomycin (P/S) was purchased from Sigma-Aldrich (St. Louis, MO)
10
Differentiation of Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MSC were induced to differentiate into adipogenic and osteogenic lineages, as described previously with minor modifications [49 (link)]. For osteogenic differentiation, MSCs were plated at 1 × 105 cells/well in six-well culture plates. After 1 day of incubation, the cells were washed in PBS and then cultured in Dulbecco’s modified Eagle medium (DMEM; WELGENE, Daegu, Korea) supplemented with 0.1 μmol/L of dexamethasone, 100 μmol/L of L-ascorbic acid 2-phosphate, 10 mmol/L of β-glycerol phosphate, and 10% FBS. For adipogenic differentiation, MSCs were plated at 5 × 105 cells/well in six-well culture plates. After 1 day of incubation, the cells were washed in PBS and then cultured in Iscove’s modified Dulbecco’s medium (WELGENE) supplemented with 1 μmol/L of dexamethasone, 0.2 mmol/L of indomethacin, 0.5 mmol/L of 3-isobutyl-1-methylxanthine, and 10% FBS. To examine the effect of PBM on MSC differentiation, treatment was performed once per day for 14 days of induction. After the induction period, differentiation was evaluated using Oil Red O staining for adipogenesis and Alizarin Red S staining for osteogenesis. Then, the degree of differentiation was quantified using a microplate reader. In addition, the expression of differentiation markers was also analyzed by real-time RT-PCR.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!