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Kaluza

Manufactured by Beckman Coulter
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Sourced in United States, Poland, United Kingdom

Kaluza is a flow cytometry analysis software that provides advanced data visualization and analysis capabilities. It enables users to efficiently explore and interpret complex flow cytometry data.

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Lab products found in correlation

Gallios flow cytometer, by Beckman Coulter (19 mentions) Cytoflex, by Beckman Coulter (8 mentions) Facscalibur, by BD (7 mentions) Facsdiva, by BD (7 mentions) Navios flow cytometer, by Beckman Coulter (5 mentions) Navios, by Beckman Coulter (4 mentions) Cytoflex s, by Beckman Coulter (4 mentions) Cellquest, by BD (4 mentions) Facscanto 2, by BD (4 mentions) Cytexpert, by Beckman Coulter (3 mentions) Celltrace violet, by Thermo Fisher Scientific (3 mentions) Facsdiva software, by BD (3 mentions) Attune nxt, by Thermo Fisher Scientific (3 mentions) Anti cd45, by Thermo Fisher Scientific (2 mentions) Anti siglecf, by BD (2 mentions) Anti ccr3, by BD (2 mentions) Facscanto, by BD (2 mentions) Tgf β1, by Thermo Fisher Scientific (2 mentions) α sma, by BD (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Kaluza C Analysis Software Kaluza Analysis software v.1.3 Kaluza version 1.5a Kaluza Flow Cytometry Analysis Software v1.1 Kaluza 2.1 analysis software Kaluza Analysis Software v2.1 Kaluza analysis program (version 1.2 Kaluza 2.1 software Kaluza software package Kaluza software version 1.5a

133 protocols using kaluza

1

Immunophenotyping and Apoptosis Assay

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For immunotypisation, splenocytes were stained with fluorophore-coupled primary antibodies against CD23 PE, (Ebioscience, clone B3B4), CD93 (PE-Cy7, Biolegend, clone AA4.1), CD21/35 (APC, BD, clone 7G6), CD45 (APC-Cy7, BD, clone 30-F11), B220 (Pacific-Blue, BD, clone RA3-6B2), CD138 (PE-Cy7, Biolegend, Cat. No. 142514), MHCII (AF700, Biolegend, clone M5-1142) and measured on a Gallios flow cytometer (Beckman Coulter). Data was analyzed with the software Kaluza (Beckman Coulter). The gating strategy is depicted in Fig. S8.
For annexin V/PI measurements, cells were cultured at an initial density of 106 cells/ml and treated with the indicated compounds and doses for 48 hours. Cells were then washed and stained with a FITC-coupled antibody against annexin V (diluted 1:600, BD, Cat. No. 550474) and propidium iodide (0.5 mg/ml). Cells were analyzed on a flow cytometer (Gallios, Beckman Coulter) after 15 minutes of incubation and the double-positive population was measured using the software Kaluza (Beckman Coulter).
Flümann R., Rehkämper T., Nieper P., Pfeiffer P., Holzem A., Klein S., Bhatia S., Kochanek M., Kisis I., Pelzer B.W., Ahlert H., Hauer J., da Palma Guerreiro A., Ryan J.A., Reimann M., Riabinska A., Wiederstein J., Krüger M., Deckert M., Altmüller J., Klatt A.R., Frenzel L.P., Pasqualucci L., Béguelin W., Melnick A.M., Sander S., Montesinos-Rongen M., Brunn A., Lohneis P., Büttner R., Kashkar H., Borkhardt A., Letai A., Persigehl T., Peifer M., Schmitt C.A., Reinhardt H.C, & Knittel G. (2020). An Autochthonous Mouse Model of Myd88- and BCL2-Driven Diffuse Large B-cell Lymphoma Reveals Actionable Molecular Vulnerabilities. Blood Cancer Discovery, 2(1), 70-91.
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2

Isolation and Quantification of Mouse Plasma Microvesicles

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Mouse venous blood was collected by retro-orbital puncture under anaesthesia in 0.129 mol/L sodium citrate (ratio of blood to anticoagulant 4:1). Samples were centrifuged at 1500xg for 15 min at room temperature. Harvested, platelet-poor plasma samples were further centrifuged at 18,000xg for 5 min, twice, to achieve platelet-free plasma. Platelet-free plasma was centrifuged at 18,000xg for 45 min, the supernatant removed and retained, and the pellet centrifuged for a further 45 min at 18,000xg in a solution of sodium citrate and PBS (ratio of citrate to PBS 1:3) to pellet MV. Total MV numbers were quantified by detection of phosphatidylserine using FITC-Annexin V labelling (Beckman Coulter, Brea, CA), as previously described41 (link). Samples were acquired on a Gallios flow cytometer (Beckman Coulter) and analysed using Kaluza (Beckman Coulter).
Niewold P., Cohen A., van Vreden C., Getts D.R., Grau G.E, & King N.J. (2018). Experimental severe malaria is resolved by targeting newly-identified monocyte subsets using immune-modifying particles combined with artesunate. Communications Biology, 1, 227.
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3

Flow Cytometric Evaluation of Transduction Efficiency

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Transduction efficiency was evaluated from 5 days post-transduction. Cells were washed twice in cold PBS, incubated with 1 µg/ml biotinylated protein L (Pierce) for 45 min at 4°C. Cells were washed twice with PBS, stained with Live/dead near-infrared for 5 min at RT, washed with PBS 0.5% FBS and stained for 20 min at RT with streptavidin-PE/BV421 and anti-CD3, -CD4, -CD8, -TCRVβ11 and -TCRVα24-Jα18. Cells were acquired the same day using a BD Fortessa and analysed using Kaluza (Beckman Coulter).
Rowan A.G., Ponnusamy K., Ren H., Taylor G.P., Cook L.B, & Karadimitris A. (2023). CAR-iNKT cells targeting clonal TCRVβ chains as a precise strategy to treat T cell lymphoma. Frontiers in Immunology, 14, 1118681.
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4

PBMC Immunofluorescence Staining Protocol

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Unstimulated PBMCs were stained after thawing from storage in liquid nitrogen. For immunofluorescence staining, PBMCs were washed with PBS containing 1% FBS, 0.5% BSA, and 0.05% sodium azide (PBA). Subsequently, PBMC were stained with directly labelled antibodies (Supplementary Table 3) and incubated for 40 minutes in the dark at 4˚C. After washing, the samples were analysed using a CytoFLEX flow cytometer. Flow cytometry data were analysed using CytExpert or Kaluza software (both from Beckman Coulter).
Fernández-Soto D., García-Jiménez Á.F., Casasnovas J.M., Valés-Gómez M, & Reyburn H.T. (2024). Elevated levels of cell-free NKG2D-ligands modulate NKG2D surface expression and compromise NK cell function in severe COVID-19 disease. Frontiers in Immunology, 15, 1273942.
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5

Phospho-ERK1/2 Activation in Eosinophils

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Total bone marrow cells were stained with anti-CD45 (ebioscience), anti–Siglec-F (BD Bioscience) and anti-CCR3 (BD Bioscience). The mature eosinophils (triple positive) were sort by MoFlo XDP (Beckman Coulter). The cells were activated with bronchoalvelolar lavage fluid (BALF) which was obtained from the lungs of CC10-Il13Tg/Pirb+/+ mice for the indicated time points (0, 5 and 10 minutes), and cells were fixed in 4% paraformaldehyde/PBS and permeabilized using saponin-based permeabilization buffer (X1, invitrogen). Cells were stained with phospho-ERK1/2 (Cell signaling). Events were acquired by FACSCanto (BD Bioscience), and data were analyzed using the Kaluza (BeckmanCoulter) or FlowJo (TreeStar) software. For phosphoflow analysis, the mean fluorescence intensity (MFI) for each time point, in each biological repeat, was normalized to baseline and expressed as the fold change over baseline.
Ben Baruch-Morgenstern N., Mingler M.K., Stucke E., Besse J.A., Wen T., Reichman H., Munitz A, & Rothenberg M.E. (2016). Paired immunoglobulin-like receptor B inhibits IL-13–driven eosinophil accumulation and activation in the esophagus. Journal of immunology (Baltimore, Md. : 1950), 197(3), 707-714.
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6

TGF-β1 Induced Myofibroblast Activation

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cMSCs and kPSCs of three different donors were seeded in a density of 200.000 cells per well in a 6 wells plate (costar) and stimulated for 48 hours with 10 ng/ml TGF-β1 (PreproTech, London, United Kingdom). Afterwards, cells were trypsinized, permeabilized with 0.1% saponin and labelled with α-SMA (BD Bioscience). α-SMA expression was analysed with flow cytometry (Accuri C6, BD Bioscience) and mean fluorescent intensities were calculated (Kaluza, Beckman Coulter, Indianapolis, USA).
Leuning D.G., Engelse M.A., Lievers E., Bijkerk R., Reinders M.E., de Boer H.C., van Kooten C, & Rabelink T.J. (2017). The human kidney capsule contains a functionally distinct mesenchymal stromal cell population. PLoS ONE, 12(12), e0187118.
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7

Multiparameter Flow Cytometry of Immune Cells

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Flow cytometric analysis of bone marrow, peripheral blood cells or enzymatically digested esophagus was conducted using the following antibodies: anti-CD11b (R&D), anti–GR-1 (BD Bioscience), anti–Siglec-F (BD Bioscience), anti-CCR3 (BD Bioscience), anti–PIR-A/B (ebioscience), IgG2b (ebioscience), anti-CD45 (ebioscience) and anti-CD11c (BD Bioscience). Cell counts were conducted using 123count beads (ebioscience) according to the manufacturers’ instructions. In all experiments, at least 50,000 events were acquired by (FACSCalibur, BD Bioscience), and data were analyzed using the Kaluza (BeckmanCoulter) or FlowJo (TreeStar) softwares.
Ben Baruch-Morgenstern N., Mingler M.K., Stucke E., Besse J.A., Wen T., Reichman H., Munitz A, & Rothenberg M.E. (2016). Paired immunoglobulin-like receptor B inhibits IL-13–driven eosinophil accumulation and activation in the esophagus. Journal of immunology (Baltimore, Md. : 1950), 197(3), 707-714.
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8

Annexin V Apoptosis Detection

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Apoptotic cells were detected using Annexin V Apoptosis Detection Set PE-CY7 (eBioscience, San Diego, CA) according to manufacturer’s instructions. The cells were acquired by a Beckman-Coulter Gallios flow cytometer and analyzed by Kaluza (Beckman-Coulter, Brea, CA).
Rozenberg P., Reichman H., Zab-Bar I., Itan M., Pasmanik-Chor M., Bouffi C., Qimron U., Bachelet I., Fulkerson P.C., Rothenberg M.E, & Munitz A. (2017). CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production. Scientific Reports, 7, 5922.
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9

Porcine and Human Leukocyte Analysis

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Files from human samples were analyzed by the internal Cell-Dyn Sapphire algorithm according to pre-set gates for different leukocyte subsets. Data from porcine samples were extracted and analyzed with manually set gates (Kaluza, Beckman Coulter, Brea, CA, USA), since the incorporated pre-set gates of the hematological analyzer were not suitable for porcine circulating cells. Data are expressed as means ± standard deviations unless mentioned otherwise. Groups were compared using a one-way ANOVA in case of more than two groups, a Student’s t test for unrelated measurements in case of comparisons of two separate groups and a paired Student’s t test for related measurements. Correlations were tested with Pearson’s correlation test.
van Hout G.P., van Solinge W.W., Gijsberts C.M., Teuben M.P., Leliefeld P.H., Heeres M., Nijhoff F., de Jong S., Bosch L., de Jager S.C., Huisman A., Stella P.R., Pasterkamp G., Koenderman L.J, & Hoefer I.E. (2015). Elevated mean neutrophil volume represents altered neutrophil composition and reflects damage after myocardial infarction. Basic Research in Cardiology, 110(6), 58.
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10

Comprehensive Flow Cytometry and Imaging Analysis

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FC analyses were performed using Kaluza (Beckman Coulter) and FlowJo (Becton Dickinson) software. All data representing FVIII were calculated after subtracting the relative immunoglobulin G. ZF images were analyzed using ZEN software (Zeiss), and cell mass size measurements were acquired using Danioscope. Descriptive and inferential (ie, t test and 1-way analysis of variance) statistical analyses were performed using GraphPad Prism.
Elnaggar M., Al-Mohannadi A., Hasan W., Abdelrahman D., Al-Kubaisi M.J., Pavlovski I., Gentilcore G., Sathappan A., Kizhakayil D., Ali A.I., Mohan S., Olagunju D., Cugno C., Grivel J.C., Borsotti C., Follenzi A., Da’as S.I, & Deola S. (2022). CD14+/CD31+ monocytes expanded by UM171 correct hemophilia A in zebrafish upon lentiviral gene transfer of factor VIII. Blood Advances, 7(5), 697-711.
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