Chitosan

Manufactured by Maclin Biochemical Technology

Sourced in China, Ireland

Chitosan is a polysaccharide derived from the shells of crustaceans, such as shrimp and crab. It is a natural, biodegradable, and biocompatible material with a wide range of applications in various industries, including biomedical, pharmaceutical, and agricultural sectors. Chitosan exhibits unique properties, such as antimicrobial activity, wound healing enhancement, and the ability to form films and hydrogels.

Automatically generated - may contain errors

Lab products found in correlation

Laminarin, by Maclin Biochemical Technology (18 mentions) Xylan, by Maclin Biochemical Technology (18 mentions) Avicel, by Maclin Biochemical Technology (18 mentions) Barley β glucan, by Maclin Biochemical Technology (18 mentions) Carboxymethyl cellulose, by Maclin Biochemical Technology (9 mentions) Trichloroacetic acid, by Maclin Biochemical Technology (9 mentions) Carrageenan, by Maclin Biochemical Technology (9 mentions) Lichenin, by Megazyme (6 mentions) Escherichia coli dh5α, by Thermo Fisher Scientific (3 mentions) Pluronic f68, by BASF (3 mentions) Pet 29a, by Takara Bio (3 mentions) Fecl2 4h2o, by Tianjin Damao (3 mentions) Cacl2, by Guangzhou Chemical (3 mentions) Na2co3, by Guangzhou Chemical (3 mentions) Potassium chloride (kcl), by Guangzhou Chemical (3 mentions) Mgcl2, by Guangzhou Chemical (3 mentions) Na2hpo4, by Guangzhou Chemical (3 mentions) Sodium chloride (nacl), by Guangzhou Chemical (3 mentions) Na2so4, by Guangzhou Chemical (3 mentions) Cholesterol, by Tianjin Guangfu (3 mentions)

Close spelling variants of this product

Carboxymethyl chitosan (CMCS, Carboxymethyl chitosan Chitosan (CS)

64 protocols using chitosan

Microbial Enzyme Production and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Barley β-glucan (80% purity), laminarin, carboxymethyl cellulose (CMC), xylan, curdlan, avicel, and chitosan were purchased from Shanghai McLean Biochemical Technology Co., Ltd. Lichenin was purchased from Megazyme (Ireland).
Bacillus subtilis B110 strain was acquired from the Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources of Jiangxi Province and maintained in a lysogeny broth. Escherichia coli DH5α (Invitrogen Co., China) was used as the host strain for gene manipulation. E. coli BL21 (DE3) (Novagen Co., China) was used for recombinant expression. Plasmid pET-29a (TakaRa Biotechnology, China) served as the expression vector.

Huang Z., Ni G., Wang F., Zhao X., Chen Y., Zhang L, & Qu M. (2021). Characterization of a Thermostable Lichenase from Bacillus subtilis B110 and Its Effects on β-Glucan Hydrolysis. Journal of Microbiology and Biotechnology, 32(4), 484-492.

+ Open protocol

+ Expand

Crayfish Antimicrobial Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Live crayfish with an average weight of 30

\pm

2.4 g and an average length of 20

\pm

1.8 cm were purchased from Qianjiang Changgui Aquatic Food Co., Ltd. (Nantong, China). Four strains of bacteria, including Escherichia coli (AB93154), Shewanella putrefaciens (ATCCBAA-1097), Staphylococcus aureus (ATCC43979), and Salmonella typhimurium (ATBA45479), were purchased from the Shanghai Preservation Biotechnology Centre, (Shanghai, China). All other reagents (chitosan, lysozyme, carrageenan, MgO, KBr, trichloroacetic acid, etc.) used in this study were analytically pure and were purchased from McLean Biochemical Technology Co. Ltd. (Shanghai, China).

Qiu L., Luo Q., Bai C., Xiong G., Jin S., Li H, & Liao T. (2023). Preparation and Characterization of a Biodegradable Film Using Irradiated Chitosan Incorporated with Lysozyme and Carrageenan and Its Application in Crayfish Preservation. Foods, 12(14), 2642.

+ Open protocol

+ Expand

Chitosan-Silane Composite Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chitosan (CS, MW = 30,000, ≥85% deacetylation), ZnCl₂, and NaBr (analytical purity) were purchased from Shanghai Maclean Biochemical Technology Co. (Shanghai, China). Triethoxy (3-epoxypropoxy) silane (KH-561, purity > 96.0%) and tannin (analytical purity) were purchased from Aladdin Reagent Co. (Shanghai, China). Hydrochloric acid, MgSO₄, and KCl (analytical purity) were purchased from Yantai Shuang Chemical Co. (Yantai, China). Tetrahydrofuran (THF, analytical purity), methanol (analytical purity), acetic acid (analytical purity), and AlCl₃ (analytical purity) were purchased from Tianjin Tianli Chemical Reagent Co. (Tianjin, China). MnCl₂ (analytical purity) was purchased from Tianjin Xinput Chemical Co. (Tianjin, China). NaCl, CaCl₂, and MgCl₂ (analytical purity) were purchased from Tianjin Hengxing Chemical Reagent Manufacturing Co. (Tianjin, China). NaHCO₃ (analytical purity) was purchased from Tianjin Dayong Chemical Reagent Manufacturing Co. (Tianjin, China).

Liu Y., Ma M., Shen Y., Zhao Z., Wang X., Wang J., Pan J., Wang D., Wang C, & Li J. (2024). Polyhedral Oligomeric Sesquioxane Cross-Linked Chitosan-Based Multi-Effective Aerogel Preparation and Its Water-Driven Recovery Mechanism. Gels, 10(4), 279.

+ Open protocol

+ Expand

Chitosan-Keratin Hydrogel with Curcumin for Wound Healing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chitosan (Mw 50 kDa, >98%) was purchased from Shanghai Maclean Biochemical Technology Co., Ltd. (Shanghai, China). (3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCL) and N-hydroxysuccinimide (NHS) were purchased from Nanjing dulai Biotechnology Co., Ltd. (Nanjing, China). Keratin was obtained from Shouhe Biotechnology Co., Ltd. (MW1500, Xi’an, China). Curcumin (≥98%) was purchased from Ivy Biotechnology Co., Ltd. (Xian, China). Polyethylene glycol 400 (PEG400) and glycerol octanoate decanoate (GTCC) were purchased from Shandong Yousuo Chemical Technology Co., Ltd. (Linyi, China). Pluronic F68 was purchased from BASF Ltd. (Shanghai, China). 4′,6-diamidino-2-phenylindole (DAPI), haematoxylin and eosin were purchased from Sigma-Aldrich (Shanghai, China). All other reagents were analytical grade.

Niu J., Yuan M., Gao P., Wang L., Qi Y., Chen J., Bai K., Fan Y, & Liu X. (2023). Microemulsion-Based Keratin–Chitosan Gel for Improvement of Skin Permeation/Retention and Activity of Curcumin. Gels, 9(7), 587.

+ Open protocol

+ Expand

Cellulose-degrading Enzyme Discovery

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glucose and other cello-oligosaccharide (G₂-G₅), carboxymethyl-cellulose (CMC), barley β-glucan, laminarin, xylan (from corncob), avicel, chitosan, and filter paper were obtained from McLean Biochemical Technology Co., Ltd. (Shanghai, China), whereas lichenin was purchased from Megazyme (Bray, Ireland). Other chemicals used in this study were provided by Sigma-Aldrich (Shanghai, China).
Myxococcus sp. B6-1 was isolated from soil and cultured in a lysogeny broth (LB). DH5 and BL21 (DE3) strains of E. coli from our laboratory were respectively utilized for gene cloning and expression. Plasmid pET-29a (Takara, Beijing, China) was used for expression.

Huang Z., Ni G., Dai L., Zhang W., Feng S, & Wang F. (2023). Biochemical Characterization of Novel GH6 Endoglucanase from Myxococcus sp. B6-1 and Its Effects on Agricultural Straws Saccharification. Foods, 12(13), 2517.

+ Open protocol

+ Expand

Cationic Polymer-Based Dye Adsorption

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium lignosulfonate (C₂₀H₂₄Na₂O₁₀S₂,
MW 534.51 g/mol, 96%), chitosan (C_6nH_11nNO_4n, 95% deacetylated,
viscosity 100–200 mPa s), Rhodamine B (C₂₈H₃₁ClN₂O₃, MW 479.01 g/mol, AR), and Congo
red (C₃₂H₂₂N₆Na₂O₆S₂, MW 696.66 g/mol, BS) were obtained from Shanghai
Macklin Biochemical Co., Ltd. Potassium dichromate (K₂Cr₂O₇, MW 294.18 g/mol, GR) was provided by Sinopharm
Chemical Reagent Co., Ltd. Acetic acid (CH₃COOH, MW 60.05
g/mol, AR) was purchased from Beijing Chemical Works. In addition,
all other solutions were prepared by distilled water. All chemicals
in the experiments were used without further purification.

Gu F., Geng J., Li M., Chang J, & Cui Y. (2019). Synthesis of Chitosan–Ignosulfonate Composite as an Adsorbent for Dyes and Metal Ions Removal from Wastewater. ACS Omega, 4(25), 21421-21430.

+ Open protocol

+ Expand

Acellular Dermal Matrix-Chitosan Composite Scaffold

Check if the same lab product or an alternative is used in the 5 most similar protocols

The acellular dermal matrix was prepared by the hypertonic salt-alkali method using the full-thickness skin of white Tibetan miniature pigs as raw material. The solutions containing 0.5%, 1%, and 2% acetic acid were designed. The PADMS and acetic acid solutions were added in a mass ratio of 1:10. In a beaker, stirred at 1500 rpm for 48 hours, filtered to form an acellular dermal matrix gel (group marked as 0.5%, 1%, and 2%), and stored at 4°C for later use. Acellular dermal matrix gel (group marked as 10% PADM) was mixed with 3% chitosan (deacetylation degree ≥95%, viscosity 100∼200 Mpa.s, Macklin) solution in different volumes ratios (group marked as CADMS) and cross-linked with glutaraldehyde solution. The acellular dermal matrix-chitosan composite gel was prepared with a 0.02 V/v.% cross-linking agent (group labeled as CADMS-G). The prepared acellular dermal matrix-chitosan composite gels (groups marked as 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 2:1, 3:1, 4:1, 5:1). The above gel was freeze-dried to prepare a composite sponge scaffold, soaked in a 70% ethanol solution for 1 hour to remove residual acetic acid, thoroughly washed, and then freeze-dried again for subsequent characterization of physical and chemical properties.

Chen L., Huang C., Zhong Y., Chen Y., Zhang H., Zheng Z., Jiang Z., Wei X., Peng Y., Huang L., Niu L., Gao Y., Ma J, & Yang L. (2022). Multifunctional sponge scaffold loaded with concentrated growth factors for promoting wound healing. iScience, 26(1), 105835.

+ Open protocol

+ Expand

Chitosan-based Antimicrobial Scaffold Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The carrier used for the experiments was Na-MMT (pharmaceutical grade, Zhejiang Fenghong Ltd. (Hangzhou, China). LP0042 tryptone, LP0021 yeast extract, and CM0367 agar were purchased from Thermo Fisher Scientific Ltd. (Waltham, MA, USA). Chitosan (100 kDa, ≥95% deacetylation) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Silver nitrate, sodium chloride, sodium hydroxide, etc., were analytical pure reagents provided by Tianjin Tiantai Fine Chemicals Ltd. (Tianjin, China). and Yantai Shuang Shuang Chemical Ltd. (Yantai, China). Wistar rats were purchased from Qingdao Qinda Biotechnology Ltd. (Qingdao, China). MC3T3-E1 cells were purchased from iCell Bioscience Inc. (Shanghai, China). S. aureus strains were self-stored and activated in the laboratory.

Yang K., Shen L., Zhang L., Sun W., Zou Y., Ren Y, & Zeng R. (2023). Antibacterial Activity and Biocompatibility of Ag-Montmorillonite/Chitosan Colloidal Dressing in a Skin Infection Rat Model: An In Vitro and In Vivo Study. Journal of Functional Biomaterials, 14(9), 470.

+ Open protocol

+ Expand

Chitosan-Genipin Hydrogel Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For material synthesis, sodium oleate (C₁₈H₃₃NaO₂, chemical pure), calcium chloride (CaCl₂, 96.0%), sodium dihydrogen phosphate (NaH₂PO₄, 99.0%), hexane (C₆H₁₄, 97.0%), ethanol (C₂H₆O, 99.7%), acetic acid (C₂H₄O₂, 99.5%) and sodium hydroxide (NaOH, 96.0%) were purchased from Sinopharm Chemical Reagent Co., Ltd. Chitosan (C_6nH_11nNO_4n, deacetylation degree ≥95%) and genipin (C₁₁H₁₄O₅, 98%) were purchased from Shanghai Macklin Biochemical Co., Ltd. All of the chemicals were used without further purification.
For the cell experimental process, the α−minimum essential medium (α−MEM), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) and penicillin/streptomycin were purchased from Gibco (America). Calcein acetoxymethyl ester (calcium AM) and propidium iodide (PI) were purchased from Beijing Solarbio Science and Technology Co., Ltd. Cell counting kit-8 (CCK-8) was purchased from Dojindo (Japan). FITC-phalloidin was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from Abcam (United Kingdom). Alizarin Red S was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (China).

Xia H., Dong L., Hao M., Wei Y., Duan J., Chen X., Yu L., Li H., Sang Y, & Liu H. (2021). Osteogenic Property Regulation of Stem Cells by a Hydroxyapatite 3D-Hybrid Scaffold With Cancellous Bone Structure. Frontiers in Chemistry, 9, 798299.

+ Open protocol

+ Expand

Chitosan-based Nitrogen-doped Carbon Composites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chitosan and NH₄Cl were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). All reagents used in this study were analytically pure, and no further purification was required prior to use.
Pretreatment of Chitosan: an appropriate amount of Chitosan was soaked in an oxalic acid solution with a mass ratio of 2 wt.% for 1 h. The solution was then rinsed with ultrapure water until neutral, and the remaining powder was placed in a vacuum drying oven and dried at 40–50 °C for 12 h. The light-yellow powder was subsequently obtained by grinding.
Preparation of NCC materials: Firstly, the mixture of 10 g of pretreated sugar and NH₄Cl was ground for 10 min and heated to 1000 °C in a N₂ tube furnace and then held for 3 h until cooled to room temperature, and the black swelling was collected and ground into a black powder in an agate mortar, which is the NCC powder, as shown in Figure 7.

Zhang M., Song H., Ma Y., Yang S, & Xie F. (2023). Preparation of NH4Cl-Modified Carbon Materials via High-Temperature Calcination and Their Application in the Negative Electrode of Lead-Carbon Batteries. Molecules, 28(14), 5618.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!