The gastric tissue was fixed using 4% formalin overnight and subsequently embedded in paraffin. To prepare the sections for analysis, 5 μm thick sections were deparaffinized using a series of graded alcohols and isopropanol. The sections were then subjected to H&E staining using a commercial H&E staining kit (Beyotime). Briefly, the sections were stained with hematoxylin solution for 6 min and followed by treatment with 1% acid alcohol for 2 s. Subsequently, the sections were stained with eosin for 2 min using the eosin solution provided in the H&E staining kit (C0105, Beyotime).
He staining kit
Manufactured by Beyotime
Sourced in China
The HE staining kit is a laboratory tool used for the basic staining and visualization of tissue samples. It contains the necessary reagents and protocols for performing hematoxylin and eosin (H&E) staining, a widely used histological technique.
Automatically generated - may contain errors
Lab products found in correlation
Masson staining kit, by Solarbio (4 mentions)
Optical microscope, by Olympus (3 mentions)
Cebpb, by Santa Cruz Biotechnology (3 mentions)
Ix81 microscope, by Olympus (3 mentions)
Eclipse 80i, by Nikon (3 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (3 mentions)
Balb c nude mice, by Charles River Laboratories (2 mentions)
Matrigel, by BD (2 mentions)
Optical microscope, by Leica (2 mentions)
Image pro plus 7, by Media Cybernetics (2 mentions)
Digital pathology slide scanner, by 3DHISTECH (2 mentions)
Pas staining kit, by Merck Group (2 mentions)
Modified masson s trichrome stain kit, by Solarbio (2 mentions)
Ab40815, by Abcam (2 mentions)
Ab14686, by Abcam (2 mentions)
Hbred090bc01, by Shanghai Outdo Biotech Co. (2 mentions)
Fluorescence microscope, by Olympus (1 mentions)
G2161, by Solarbio (1 mentions)
Pyruvate, by Merck Group (1 mentions)
Anti phospho creb ser133, by Cell Signaling Technology (1 mentions)
173 protocols using he staining kit
1
Histological Examination of Gastric Tissue
2
Nude Mice Tumor HE Staining
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Nude mice were divided into three groups: a control group, a vector group, and a miR-588 overexpression group. The tumor tissue of those groups was used for HE staining. The HE staining kit was bought from Beyotime. Procedures were followed according to the HE staining kit instructions (Beyotime, C0105).
3
Establishing Lung Cancer Xenograft and Metastasis Models
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The Research Ethics Committee of Second People’s Hospital of Gansu Province approved the animal experiments (approval number: 201908). Thirty BALB/c nude mice (4 weeks old, 20 ± 2 g) were from Vital River (Beijing, China). A549 and PC13 cells (1 × 106) were diluted in 200 μL PBS and 200 μL Matrigel (BD Biosciences) under sterile conditions and injected subcutaneously into anesthetized nude mice to establish a lung cancer xenograft model. The tumor volume of mice was measured at an interval of 4 days after injection of lung cancer cells, and the mice were euthanized 16 days later by an overdose of pentobarbital sodium (120 mg/kg). The tumor volume was calculated as 1/2 × the long axes × the short axes2 [22 (link)].
Thirty BALB/c nude mice were euthanized by an overdose of pentobarbital sodium at 120 mg/kg 45 days after tail vein injection of 1 × 106 A549 and PC13 cells. Liver tissues were extracted for hematoxylin-eosin (HE) staining to observe the formation of metastases. HE staining kits were from Beyotime (Shanghai, China). The liver tissues were preserved after conventional paraffin embedding. The liver sections were dewaxed, stained with hematoxylin for 5 min, differentiated with 1% ethanol hydrochloride for 3 s, and stained with 5% eosin for 3 min. Observation of tissue sections was conducted under a fluorescence microscope (Olympus).
Thirty BALB/c nude mice were euthanized by an overdose of pentobarbital sodium at 120 mg/kg 45 days after tail vein injection of 1 × 106 A549 and PC13 cells. Liver tissues were extracted for hematoxylin-eosin (HE) staining to observe the formation of metastases. HE staining kits were from Beyotime (Shanghai, China). The liver tissues were preserved after conventional paraffin embedding. The liver sections were dewaxed, stained with hematoxylin for 5 min, differentiated with 1% ethanol hydrochloride for 3 s, and stained with 5% eosin for 3 min. Observation of tissue sections was conducted under a fluorescence microscope (Olympus).
4
Histopathological Evaluation of Tumor Tissues
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HE staining kits (C0105, Beyotime, Shanghai, China) were used for the staining. In brief, the subcutaneously transplanted tumor tissues were fixed with 10% neutral buffered formaldehyde (G2161, Solarbio, Beijing, China) at 4 ºC for 24 h, The tissues were then dehydrated, paraffin-embedded and sectioned, conventionally dewaxed with xylene and hydrated with gradient alcohol. Hematoxylin was used to stain the sections for 5–10 min, followed by eosin staining solution for 30 s-2 min. Finally, the sections were dehydrated with gradient alcohol, cleared with xylene, sealed with neutral gum, photographed and observed under an inverted microscope (Olympus, IX73) [31 (link)].
5
Histological Effects of Risperidone on Mice Femur
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Following feeding with a wet-mash containing Risperidone (Hebei Cangzhou Enke Pharmaceutical Technology Co., Ltd., Hebei, China) at different doses, the mice were euthanized by cervical dislocation to collect left femur tissues and the excess tissues were removed. The obtained tissues were then fixed in 10% neutral buffered formalin at room temperature for 48 h, decalcified with 10% ethylene diamine tetraacetic acid (EDTA) at room temperature for 2 weeks, dehydrated with 70% ethanol and paraffin-embedded. Subsequently, the paraffin-embedded tissues were sectioned at a thickness of 5 μm using a slicer and stained with HE Staining kits (C0105, Beyotime, Shanghai, China) as per the manufacturer’s instructions. Three sections from 5 mice in each group were selected and observed under an inverted microscope (IX73, Olympus Optical Co., Ltd., Tokyo, Japan), with the representative images regarded as the experimental results.
6
Hematoxylin and Eosin Staining Protocol
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According to H&E staining kits (C0105, Beyotime), the tissue samples were dewaxed in xylene, hydrated with gradient ethanol, stained with hematoxylin (5–10 minutes), and with eosin (30 s to 2 minutes). After that, the samples were observed under a microscope (Olympus IX 71, Olympus).
7
Histopathological Analysis of Rat Myocardium
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Rat myocardial tissues were stained with hematoxylin and eosin (HE) staining kits (C0105M, Beyotime, Shanghai, China) and Masson staining kits (G1340, Solarbio, Beijing, China). The tissues were fixed in neutral formalin solution (10%), paraffin-embedded, and sectioned into 3 µm on an ultramicrotome. The sections were then dewaxed in xylene and hydrated in gradient ethanol. Next, the sections were washed with tap water, followed by HE staining and Masson staining as per the instructions. The sections were washed in tap water, dehydrated in the order of 70%, 80%, 90%, and anhydrous ethanol (5 min for each) at room temperature, and cleared with xylene twice for 5 min each. Then, the sections were sealed with neutral gum or other sealer and examined under a microscope (Zeiss, Oberkochen, Germany) to observe the arrangement of cardiomyocyte nuclei, cardiomyocyte area, and collagen fiber area. The cardiac myocyte cross-sectional area analysis was performed on HE staining images with Image J 6.0 image analysis software. The collagen fiber area was analyzed in Masson staining images.
8
Tissue Preparation and Histological Staining
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Lung tissues were collected, rinsed with normal saline, drained with filter paper, and sliced into about 1.5-cm pieces. Next, the tissues were fixed with 4% paraformaldehyde, dehydrated, cleared, paraffin-embedded, and sectioned using a slicing machine. Subsequently, the tissue sections were dewaxed, soaked in gradient alcohol, dehydrated, cleared, and sealed with neutral gum. Under a microscope, the nucleus was observed to exhibit blue coloration, and the cytoplasm exhibited pink or red coloration. The staining process was performed according to the instructions of HE staining kits (C0105, Shanghai Beyotime Biotechnology Co. Ltd., Shanghai, China).
9
Investigating FGF21 Signaling Pathways
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Pyruvate, Compound C, GW6471, forskolin, IBMX, 8-Bromo-cAMP, H89, ESI-09, 666-15 and MTT were purchased from Merck KGaA (Darmstadt, Germany). Dulbecco’s modified eagle medium (DMEM), fetal bovine serum (FBS) and trypsin for cell culture were obtained from Thermo Fisher (Waltham, MA, USA). The kits for RNA extractive, reverse transcription (RT) and quantitative PCR (qPCR) were obtained from Takara Bio Inc (Dalian, China). Protein extraction kits and BCA assay kits were purchased from Bio-Rad Laboratories (Hercules, USA). Human and mouse FGF21 ELISA kits, cAMP assay kits, the ALT activity assay kit, the AST activity assay kit and the LDH assay kit were obtained from Elabscience (Wuhan, China). Pyruvate assay kits were obtained from Abcam (Boston, MA, USA). Anti-cAMP response element-binding protein (CREB) and anti-phospho-CREB (Ser133) antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). H&E staining kits were obtained from Beyotime Biotechnology (Beijing, China).
10
Histological Analysis of Tendon-Bone Repair
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All collected tissues were fixed in 4% paraformaldehyde solution and then decalcified in 10% EDTA decalcification solution (Sangon Biotech, Shanghai, China) for 1 month. Next, the completely decalcified samples were sequentially immersed in PBS/10% sucrose solution for 4 hours and PBS/30% sucrose solution overnight. Subsequently, the tissues were embedded in an OCT medium and sectioned for 10 μm. After being thawed at room temperature, the sections were stained with SO-FG staining kits (Solarbio, Beijing, China), TB staining (Servicebio, Wuhan, China), H&E staining kits (Beyotime Biotechnology, Shanghai), and Masson staining kits (Solarbio, Beijing, China) to analyze the repair effect of tendon and bone tissues. The images were photographed by an optical microscope (Leica, Germany). Semiquantitative analysis of the area ratio of fibrocartilage at the interface was performed using ImageJ software.
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