Apc tcrβ clone h57 597

Manufactured by BD

The APC—TCRβ (clone H57-597) is a monoclonal antibody that binds to the T-cell receptor beta chain. It is a laboratory reagent used for the identification and analysis of T cells by flow cytometry.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (1 mentions) Igm pe, by Southern Biotech (1 mentions) Anti cd43 magnetic beads, by Miltenyi Biotec (1 mentions) Pe cy5 conjugated b220, by BD (1 mentions) Anti cd40, by BD (1 mentions) Fitc conjugated igg1, by BD (1 mentions)

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TCRβ (H57-597) (11 citations) H57-597 (9 citations) TCRβ-APC (4 citations) TCRβ (clone H57-597; (3 citations)

2 protocols using apc tcrβ clone h57 597

Optimized asTCR Heterodimer Characterization

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Sequences of our asTCRs were human-codon optimized and reconstituted into the native TCR heterodimer structure. The alpha and beta chains were separated with a 2A cleavage peptide (Fig. 4a). As described above, concentrated asTCRs were titered after staining transduced J.RT3-T3.5 cells with APC—TCRβ (clone H57-597, BD Biosciences).

Amissah O.B., Chen W., de Dieu Habimana J., Sun Y., Lin L., Liu Y., Wang L., Liu Z., Mukama O., Basnet R., Liu H., Li J., Ding X., Lv L., Chen M., Liang Y., Huang R, & Li Z. (2024). NY-ESO-1-specific T cell receptor-engineered T cells and Tranilast, a TRPV2 antagonist bivalent treatment enhances the killing of esophageal cancer: a dual-targeted cancer therapeutic route. Cancer Cell International, 24, 64.

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Lymphocyte Development and CSR Analysis

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Lymphocyte development and in vitro CSR were performed as described before (10 (link),31 (link),32 ). Hematopoietic cells from 5–8 week mice were stained and analyzed on a FACS Calibur flow cytometer (BD Biosciences). The antibodies are: PE-CD4 (clone GK1.5, BD Pharmingen, 553730), FITC-CD8α (clone 53–6.7, BioLegend, 100705), APC-TCRβ (clone H57-597, BD Pharmingen, 553174), PE/Cy7 TER-119 (clone TER-119, BioLegend, 116222), FITC-CD43 (clone S7, BD Pharmingen, 553270), PE-Cy5-B220 (clone RA3-6B2, BD Pharmingen, 553091) and PE-IgM (Southern Biotech, 1020-09). For in vitro CSR, CD43⁻ splenic cells (anti-CD43 magnetic beads, Miltenyi, 130-049-801) were cultured (~1 × 10⁶ cells ml⁻¹) in RPMI (Gibco, 11875-093), serum supplements (10 (link),31 (link),32 ), IL-4 (20 ng/mL; R&D, 404-ML-050), and anti-CD40 (1 μg/mL; BD Bioscience, 553721) and analyses with FITC-conjugated IgG1 (clone A85-1, BD Pharmingen, 553443) and PECy5-conjugated B220 (clone RA3-6B2, BD Pharmingen, 553091). FlowJo software package was used for data analyses

Milanovic M., Houghton L.M., Menolfi D., Lee J.H., Yamamoto K., Li Y., Lee B.J., Xu J., Estes V.M., Wang D., Mckinnon P.J., Paull T.T, & Zha S. (2020). The cancer-associated ATM R3008H mutation reveals the link between ATM activation and its exchange. Cancer research, 81(2), 426-437.

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