Sylgard 184 elastomer

Manufactured by Dow

Sourced in United States

Sylgard 184 is a two-part silicone elastomer kit produced by Dow. It is a versatile material used for various applications that require a flexible, durable, and chemically resistant material. The kit consists of a base component and a curing agent that, when mixed, forms a silicone rubber upon curing.

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Lab products found in correlation

Sylgard 527 gel, by Dow (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Pluronic f 127, by Merck Group (3 mentions) Clampfit 10, by Molecular Devices (2 mentions) Human fibronectin, by Thermo Fisher Scientific (2 mentions) Curing agent, by Dow (2 mentions) Bovine serum albumin powder, by Bovogen (2 mentions) D glucose powder, by Bio Basic (2 mentions) Fibronectin, by Thermo Fisher Scientific (1 mentions) Bz x800 all in one fluorescent microscope, by Keyence (1 mentions) Fibronectin, by Merck Group (1 mentions) Anti adherence rinsing solution, by STEMCELL (1 mentions) Fibronectin, by Corning (1 mentions) Polycaprolactone, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Microscope cover glass, by Marienfeld (1 mentions) Thermal power sensor, by Thorlabs (1 mentions) Harris uni core, by Merck Group (1 mentions) Poly d lysine hydrobromide pdl, by Merck Group (1 mentions) Nis elements ar acquisition software, by Nikon (1 mentions)

Spelling variants of this product

Sylgard 184 (1702 citations) Sylgard (113 citations) Sylgard 184 Silicone Elastomer (92 citations) Sylgard 184 elastomer kit (50 citations) Sylgard 184 Silicone Elastomer Curing Agent (12 citations) Sylgard 184 elastomer base (4 citations) Sylgard 184 silicon elastomer base (3 citations) Sylgard® 184 silicone elastomer formulation (2 citations) Sylgard- 184 silicone elastomer plate (2 citations) PDMS rubber (Sylgard 184 silicone elastomer kit) (2 citations)

29 protocols using sylgard 184 elastomer

Intracellular Recordings of LES Muscle Oscillations

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LES muscles (see Fig. 1A) were isolated from C57BL/6 mice (2-3 months old). The final LES muscle strips used for electrophysiological experiments were 8 x 2mm. The muscle strips were pinned down in a recording chamber lined with Sylgard elastomer 184 (Dow, USA), and incubated at 37 ± 0.5°C with continuously flowing, oxygenated KRB solution for 1hr before beginning intracellular recordings. Cells in the LES were impaled with glass microelectrodes filled with 3 M KCl and having resistance 50-100 MΩ. Transmembrane potentials were measured using a high input impedance amplifier (Axopatch 2B, Molecular Devices Corp., Sunnyvale, CA, USA) and recorded with Axoscope 10.3 software. The data were analyzed by Clampfit 10.4 (Molecular Devices). All recordings were made in the presence of nicardipine (100 nM) to reduce movements and extend the durations of impalements.
LES muscles displayed continuous random oscillations of resting membrane potentials (RMP). Therefore, membrane potentials (referred to in this paper as membrane potential oscillations; MPOs) were analyzed by generating amplitude histograms from 1 min recordings using Clampfit 10.4 software (Molecular Devices). The median values and standard errors (SE) were calculated by a Gaussian Function for RMP and MPOs, respectively.

Drumm B.T., Hannigan K.I., Lee J.Y., Rembetski B.E., Baker S.A., Koh S.D., Cobine C.A, & Sanders K.M. (2022). Ca2+ signalling in interstitial cells of Cajal contributes to generation and maintenance of tone in mouse and monkey lower esophageal sphincters. The Journal of physiology, 600(11), 2613-2636.

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Isolation of Colonic Mucosal Tissue

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A 2 cm ring of tissue was cut from the healthy margin of the colonic specimen and transported from the operating theatre to the laboratory, located in the same building, in room temperature oxygenated modified Krebs solution (NaCl; 118 mM, KCl; 4.8 mM, CaCl₂; 2.5 mM, MgSO₄; 1.2 mM, NaHCO₃; 25 mM, NaH₂PO₄; 1.0 mM, glucose; 11 mM, bubbled with 95% O₂, 5% CO₂, pH 7.4). The intact ring of colonic tissue was opened up longitudinally between the two antimesenteric taenia. Tissue was then pinned out in a Sylgard-lined petri dish (Sylgard Elastomer 184, Dow, Midland, MI, United States), serosal side down, allowing the mucosa and submucosa to be removed by sharp dissection.

Johnson M.E., Humenick A., Peterson R.A., Costa M., Wattchow D.A., Sia T.C., Dinning P.G, & Brookes S.J. (2022). Characterisation of parasympathetic ascending nerves in human colon. Frontiers in Neuroscience, 16, 1072002.

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Micropatterned Substrates for Cell Culture

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Micropatterned substrates were made with standard soft lithography techniques to create a silicon master mold^{25 (link)}. PDMS was prepared by mixing Sylgard-184 elastomer and its accompanying curing agent (Dow Corning) in a 10:1 (w/w) ratio respectively. After degassing, the PDMS mixture was added over the master and cured overnight at 60 °C. Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg ml⁻¹ for 1 h. Non-adsorbed fibronectin solution was removed by blow drying with air. To prepare the substrate, coverslips were spin-coated with a 9:1 PDMS:hexane solution at a speed of 5000 rpm for 2 min and left to set overnight at room temperature. The coverslips were treated with UV-ozone immediately before placing the stamps in contact with the coated coverslip by briefly applying uniform pressure and then passivated with 0.1% Pluronic F-127 (Sigma–Aldrich) for 1 h. Stamped substrates were stored at 4 °C in 1× PBS for up to one week.

Wubshet N.H., Cai G., Chen S.J., Sullivan M., Reeves M., Mays D., Harrison M., Varnado P., Yang B., Arreguin-Martinez E., Qu Y., Lin S.S., Duran P., Aguilar C., Giza S., Clements T, & Liu A.P. (2024). Cellular mechanotransduction of human osteoblasts in microgravity. NPJ Microgravity, 10, 35.

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Micropatterned Substrates for Cell Culture

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Micropatterned substrates were made with standard soft lithography techniques to create a silicon master mold^{23 (link)}. PDMS was prepared by mixing Sylgard-184 elastomer and its accompanying curing agent (Dow Corning) in a 10:1 (w/w) ratio respectively. After degassing, the PDMS mixture was added over the master and cured overnight at 60 °C. Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 μg/ml for 1 hour. Non-adsorbed fibronectin solution was removed by blow drying with air. To prepare the substrate, coverslips were spin-coated with a 9:1 PDMS:hexane solution at a speed of 5,000 rpm for 2 minutes and left to set overnight at room temperature. The coverslips were treated with UV-ozone immediately before placing the stamps in contact with the coated coverslip by briefly applying uniform pressure and then passivated with 0.1% Pluronic F-127 (Sigma-Aldrich) for 1 hour. Stamped substrates were stored at 4 °C in 1x PBS for up to one week.

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Gastric Peristalsis Imaging and Analysis

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Gastric peristalsis was recorded by video imaging of movements of ex vivo intact stomachs and flat-sheet gastric corpus/antrum preparations in vitro. Stomachs were excised and secured in a warmed, oxygenated organ bath. After recording baseline activity of the intact stomachs (containing gastric contents), the stomachs were opened along the lesser curvature, contents were removed and the mucosa was removed by sharp dissection. The region of the stomach from the orad corpus to the pylorus was pinned as a sheet in a dish lined with Sylgard 184 elastomer (Dow Corning, Midland, MI, USA). Black surface markers (Stampenduous, Anaheim, CA, USA) were placed in a 3 × 5 or 3 × 6 (circumferential × longitudinal) grid over the serosal surface of the gastric muscles. The markers were adherent without the need for glue. The dominant contractile pattern of wild type stomachs was a continuous series of peristaltic contractions that began in the corpus and propagated to the pylorus. Video recordings were collected as previously described18 (link) using a high definition video camera (DMK31AF03, ImagingSource, Charlotte, NC, USA) connected to a computer using AstroIIDC software (ASC, Calgary, Alberta, Canada) to record QuickTime movies (.mov files) on a Mac OS-based computer (Mac, Apple Co, Cupertino, CA, USA). The tissues were constantly perfused with oxygenated KRB solution at 37°C.

Worth A.A., Forrest A.S., Peri L.E., Ward S.M., Hennig G.W, & Sanders K.M. (2015). Regulation of Gastric Electrical and Mechanical Activity by Cholinesterases in Mice. Journal of Neurogastroenterology and Motility, 21(2), 200-216.

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In Vitro Wound Healing Assay

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Silicone elastomer base (Sylgard 184 Elastomer; Dow Corning) was made per manufacturer’s instructions and cured for 96 h. Four 2 mm diameter silicone punch outs were placed directly on the bottom of a 6-well plate and spaced at least 2 mm apart. Cells were seeded around the plugs and incubated for 48 h with a media change at 24 h to achieve 95% confluency. The silicone plugs were removed following serum starvation, and the cells were rinsed with PBS to remove debris, creating a 2 mm uniform acellular area on the plate. Cells were kept in SFM or treated with 50 ng/ml of HGF and imaged using a BZ-X800 Keyence All-in-One fluorescent microscope at 4× objective. “Wounds” were visualized for 24 h by taking brightfield images every 15 min. The Keyence software was used to create videos of the wound healing over 24 h (Fig. S2). All wounds were analyzed by finding the area (μm²) every 2 to 4 h, graphing, and finding the AUC. Using the graphed data, the slope between hours 4 and 12 was calculated to find the rate of wound closure. The time it took to reach 50% confluency was found by analysis of images. GraphPad/Prism was used for statistical analysis and generating graphs (62 (link), 83 (link)).

Tarvestad-Laise K, & Ceresa B.P. (2023). Knockout of c-Cbl/Cbl-b slows c-Met trafficking resulting in enhanced signaling in corneal epithelial cells. The Journal of Biological Chemistry, 299(10), 105233.

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Soft Lithography-Enabled Protein Patterning

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Standard soft lithography techniques were used to create the silicon master mold from which polydimethoxysiloxane (PDMS) stamps were made. PDMS stamps were prepared by mixing Sylgard-184 elastomer and curing agents (Dow Corning, Midland, MI) at a ratio of 10:1 to 20:1 (w/v), casting over the mold, and curing at 50 °C overnight. Inking solution made with 40 μg/ml fibronectin (Sigma) and 20 μg/ml Alexa Flour 647 fibrinogen (Invitrogen) was added to the PDMS stamps and incubated for 1 hour at room temperature. After excess inking solution was removed and the stamp washed with deionized water and blown dry with a stream of filtered air, the stamp was placed in conformal contact with a UV-ozone treated PDMS-coated coverslip for approximately 5 s. PDMS-coated coverslips were prepared by spin-coating a layer of PDMS diluted in hexane (1:20) at 5000 rpm for 2 min ^{22 (link)}., resulted in a thickness of 72 nm ± 14 nm (n=4) as measured by a profilometer. PDMS-coated coverslips were used to promote efficient transfer of stamped proteins. After stamping was completed, the PDMS-coated coverslip was passivated with 0.1% Pluronic-F127 (Sigma) for 1 hour, washed with PBS, and kept in PBS at 4 °C for no more than 3 days before use.

Tan X., Heureaux J, & Liu A.P. (2015). Cell Spreading Area Regulates Clathrin-Coated Pit Dynamics on Micropatterned Substrate. Integrative biology : quantitative biosciences from nano to macro, 7(9), 1033-1043.

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Micropatterned Substrates for Cell Adhesion

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Micropatterned substrates were made with the standard soft lithography technique to create a silicon master mold. Polydimethylsiloxane (PDMS) was prepared by mixing Sylgard-184 elastomer and curing agent (Dow Corning) in a 10:1 (w/w) ratio. After 10 min of degassing, the PDMS mixture was poured over the master and cured overnight at 60°C. PDMS stamps with 400 µm circular patterns were incubated with fibronectin (Corning) at a concentration of 40 μg/ml for 1 h. Soft silicone substrates were UV-treated for 5 min and then immediately placed in contact with the stamps (Tan et al., 2015 (link)). Printed substrates were passivated with an anti-adherence rinsing solution (STEMCELL Technologies) for 1 h.

Cai G., Nguyen A., Bashirzadeh Y., Lin S.S., Bi D, & Liu A.P. (2022). Compressive stress drives adhesion-dependent unjamming transitions in breast cancer cell migration. Frontiers in Cell and Developmental Biology, 10, 933042.

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Fabrication of PDMS Perfusion Inserts

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The customized perfusion inserts were manufactured via casting using polydimethylsiloxane (PDMS). Briefly, SYLGARD 184 elastomer (Dow Corning, Midland, MI) base was mixed with the curing agent (Dow Corning) at a ratio of 9:1 (w/w) in a 50 ml conical centrifuge tube. After thoroughly mixing the elastomer base and curing agent using a metal spatula, the mixture was placed under vacuum to degas. The mixture was finally poured into the assembled molds, and cured for 1 hour at 60 °C. After cooling overnight, the perfusion inserts were carefully removed from the molds and cleaned.

Sladkova M., Alawadhi R., Jaragh Alhaddad R., Esmael A., Alansari S., Saad M., Mulla Yousef J., Alqaoud L, & de Peppo G.M. (2018). Segmental Additive Tissue Engineering. Scientific Reports, 8, 10895.

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Functionalized PEG Linker for Protein Conjugation

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A heterobifunctional polyethylene glycol linker of 5 kDa, referred to as COOH-PEG-SH, was purchased from JenKem Technology (Allen, TX, USA); 1-Ethyl-3-(3-dimethylamino-propyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), fluorescein isothiocyanate (FITC), gold chloride trihydrate, and ovalbumin (OVA) from Sigma-Aldrich; and sylgard 184 elastomer from Dow Corning (Midland, MI, USA). Influenza hemagglutinin (HA) protein from A/PR/8/34 strain was obtained from BEI resources, SU-8 2150 epoxy-based negative photoresist from MicroChem (Newton, MA, USA), and polyclonal rabbit anti-FITC antibody and normal control rabbit IgG from Life Technologies (Frederick, MD, USA). FITC-conjugated secondary antibodies against mouse IgG or pig IgG were acquired from BioLegend (San Diego, CA, USA) or Sigma (St Louis, MO, USA) respectively. Inactivated influenza vaccine was made by treatment of purified A/PR/8/34 virus with 0.024% formaldehyde at 4 °C for one week as previously described [21 ]. The amount of HA protein in the vaccine was quantified by SDS-PAGE following a standard procedure.

Li B., Wang J., Yang S.Y., Zhou C, & Wu M.X. (2015). Sample-free quantification of blood biomarkers via laser-treated skin. Biomaterials, 59, 30-38.

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