The HTEC-Exos were dropped onto the copper grid and incubated for 5 min at room temperature. Then, the cells were incubated with a drop of 2% uranyl acetate for 1 min and dried for 20 min. TEM (Tecnai G2 Spirit BioTwin, FEI Company, Hillsboro, OR, USA) was used for observing the morphology of HTEC-Exos.
Tem tecnai g2 spirit bio twin
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TEM Tecnai G2 Spirit Bio Twin is a transmission electron microscope designed for high-resolution imaging and analysis of biological samples. It features a twin-lens configuration and a variety of advanced imaging modes to support a range of applications in life science research and education.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using tem tecnai g2 spirit bio twin
1
Electron Microscopy of HTEC-Exosomes
2
Phage Morphology Visualization by TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The purified phage sample was loaded onto a copper grid for 7 min followed by negative staining with 2% (vol/vol) uranyl acetate (pH 6.7) and drying. The phage morphology was observed using a FEI TEM Tecnai G2 Spirit Biotwin (FEI, Hillsboro, US) at an accelerating voltage of 80 kV.
3
Ultrastructural Analysis of Duodenum and Colon
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Duodenum and colon samples were immediately sectioned in small fragments of approximately 1 mm3 and fixed in 2.5% glutaraldehyde solution in 0.1 M phosphate buffer (pH = 7.2) for 2 h. Sequential post-fixation was performed in 1% osmium tetroxide, for 1.5 h, and 1% aqueous uranyl acetate, for 1 h in the dark. After rinsing with distilled water, samples were dehydrated in a graded acetone series (30–100%) and embedded in an Epoxy resine (Fluka Analytical, Sigma-Aldrich, Darmstadt, Germany). Ultrathin sections obtained with a Leica EM UC6 (Leica Co, Vienna, Austria) ultramicrotome were mounted on copper grids and stained with lead citrate 0.2% for 10 min. Observations were carried out on a TEM Tecnai G2 Spirit Bio Twin at 100 kV (FEI, Hillsboro, OR, USA), and images were processed using AnalySIS 3.2.
4
Ultrastructural Analysis of Tissue Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue samples (heart, liver, kidney, stomach, spleen, EWAT, small intestine and cecum) were immediately sectioned in small fragments of ~1 mm3 and fixed in 2.5% glutaraldehyde solution in 0.1 M phosphate buffer (pH=7.2) for 2 hours. Sequential postfixation was performed in 1% osmium tetroxide, for 1.5 hours, and 1% aqueous uranyl acetate, for 1 hour in the dark. After rinsing in distilled water, samples were dehydrated in a graded acetone series (30%–100%), and embedded using an Epoxy embedding kit (Fluka Analytical, Sigma-Aldrich, Germany). Ultrathin sections were obtained on a Leica EM UC6 (Leica Co, Austria) ultramicrotome and mounted on copper grids and stained with lead citrate 0.2% for 10 minutes. Observations were carried out on a TEM Tecnai G2 Spirit Bio Twin at 100 kV (FEI, Hillsboro, OR, USA), and images were processed using AnalySIS 3.2.
5
Transmission Electron Microscopy of Phage
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The purified phage was loaded onto a copper grid for 10 min, negatively stained with 2% (v/v) phosphotungstic acid (pH 6.7) and dried. The morphology of the phage was observed using a FEI TEM Tecnai G2 Spirit Biotwin (FEI, Hillsboro, US) at an accelerating voltage of 120 kV.
6
Ultrastructural Analysis of Duodenum and Colon
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Duodenum and colon samples collected after the sacrifice were immediately sectioned in small fragments of about 1 mm3 and fixed in 2.5% glutaraldehyde solution in 0.1 M phosphate buffer (pH = 7.2) for 2 h. Successive post-fixation was done in 1% osmium tetroxide (1.5 h) and in 1% aqueous uranyl acetate (1 h in the dark). After washing with distilled water, samples were dehydrated in a graded acetone series (30–100%) and embedded in an Epoxy resine (Fluka Analytical, Sigma-Aldrich, Darmstadt, Germany). Ultrathin sections obtained with a Leica EM UC6 (Leica Co, Vienna, Austria) ultramicrotome were then mounted on copper grids and stained with lead citrate 0.2% for 10 min. Observations were carried out on a TEM Tecnai G2 Spirit Bio Twin at 100 kV (FEI, Hillsboro, OR, USA), and images were processed using AnalySIS 3.2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!