The concentrations of TAF in each study were determined by HPLC (Agilent Technologies 1220 Infinity UK Ltd, Stockport, UK). The column used was a SphereClone™ C18 ODS (pore size, 80 Å; length, 150 mm; internal diameter, 4.6 mm; particle size, 5 μm) (Phenomenex, Torrance, CA, USA). The temperature of the column was fixed at 25 °C. A successful chromatographic TAF separation was achieved using a mobile phase composed of 65% ammonium acetate buffer 0.03 M (adjusted to pH 6.0 with acetic acid) and 35% of THF:ACN (70:30% v/v). The maxima absorption (λmax) was fixed at 260 nm, the injection volume was 20 μl, and the flow rate was 1 ml/min.
Sphereclone c18 ods
Manufactured by Phenomenex
Sourced in United States
SphereClone™ C18 ODS is a reversed-phase high-performance liquid chromatography (HPLC) column. It features a spherical silica-based packing material with a chemically bonded octadecyl (C18) stationary phase. The column is designed for the separation and analysis of a wide range of organic compounds.
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Lab products found in correlation
2 protocols using sphereclone c18 ods
1
HPLC Quantification of TAF
2
Quantification of Metronidazole by RP-HPLC
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MTZ quantification was performed using Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). A Phenomenex® SphereClone™ C18 ODS (150 mm length x 4.60 mm internal diameter, 5 μm particle size) column was used as the stationary phase. The mobile phase was consisted of 0.5% v/v of Triethylamine (TEA) in water (pH 6.80 adjusted using phosphoric acid) as aqueous phase and methanol as organic phase with the ratio of 60:40% v/v. The injection volume was 20 μL and the flow rate was 1.0 mL/min. The wavelength of UV detection was set at 322 nm. The measurement was carried out at 25 °C. The chromatograms obtained were analysed using Agilent ChemStation® Software B.02.01.
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