Activetwo system

Manufactured by BioSemi

Sourced in Netherlands

The ActiveTwo system is a high-performance data acquisition system designed for a wide range of biophysical measurements. It features a modular design and supports multiple input channels for recording electrical signals from various sensors and transducers. The system provides advanced signal processing capabilities and is suitable for a variety of applications in research and clinical settings.

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Lab products found in correlation

Matlab, by MathWorks (10 mentions) Actiview software, by BioSemi (8 mentions) Headcap, by BioSemi (6 mentions) Actiview, by BioSemi (5 mentions) Brain vision analyzer, by Brain Products (4 mentions) Presentation software, by Neurobehavioral Systems (4 mentions) Equiradial system, by BioSemi (4 mentions) Activetwo head cap, by BioSemi (3 mentions) Common mode sense active electrode, by BioSemi (3 mentions) Driven right leg passive electrode, by BioSemi (3 mentions) Brain vision analyzer 2, by Brain Products (3 mentions) Actiview recording software, by BioSemi (3 mentions) Actiview acquisition software, by BioSemi (3 mentions) Matlab r2016b, by MathWorks (3 mentions) Brain vision analyzer software, by Brain Products (2 mentions) Eeglab toolbox, by MathWorks (2 mentions) Drl cms circuit, by BioSemi (2 mentions) Matlab r2019b, by MathWorks (2 mentions) Eeglab v14, by MathWorks (2 mentions) Activeview acquisition software, by BioSemi (2 mentions)

Close spelling variants of this product

ActiveTwo (182 citations) ActiveTwo EEG system (38 citations) ActiveTwo amplifier system (28 citations) ActiveTwo BioSemi electrode system (5 citations) ActiveTwo 32-channel EEG system (4 citations) ActiveTwo MARK II Biosemi EEG System (4 citations) ActiveTwo AD-box EEG system (3 citations) ActiveTwo biopotential measurement system (2 citations) International ActiveTwo system (2 citations) ActiveTwo BSPM system (2 citations)

348 protocols using activetwo system

EEG Data Collection in Cognitive Experiments

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In the AM BBN experiment, 40 channels of EEG data were recorded at 16,384 Hz (analog –3-dB point of 3276.8 Hz), using a BioSemi ActiveTwo system. A total of 32 cephalic electrodes were positioned according to the standard 10–20 system. A further eight non-cephalic electrodes were also collected although only two, those over the left and right mastoids, were used in the analysis. Triggers indicating the start of each 60-s trial were encoded in a separate channel in the stimulus WAV file as three cycles of a 16-kHz tone burst. These triggers were interpreted by custom hardware before being fed into the acquisition laptop for synchronous recording along with the EEG.
In the speech experiment, 130 channels of EEG data were recorded at 512 Hz (analog –3-dB point of 409.6 Hz), using a BioSemi ActiveTwo system. 128 cephalic electrodes were positioned according to the BioSemi Equiradial system, with another two electrodes located over the left and right mastoids. Triggers indicating the start of each ∼155-s trial were presented using Neurobehavioral Systems Presentation software for synchronous recording along with the EEG.

Drennan D.P, & Lalor E.C. (2019). Cortical Tracking of Complex Sound Envelopes: Modeling the Changes in Response with Intensity. eNeuro, 6(3), ENEURO.0082-19.2019.

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Spatial Stroop Task: EEG and EMG Recording

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During the Spatial Stroop task, the EEG was recorded from 128 sites with Ag/AgCl electrodes. Horizontal electrooculograms (hEOG) were recorded from the left and right outer canthi, and vertical electrooculograms (vEOG) from above and below the left eye. These were recorded with a bandwidth of DC to 205 Hz, using a BioSemi Active Two system (BioSemi Inc.). The system uses “offset” units consisting of CMS (common mode sense) and DRL (driven right leg) electrodes that were kept below 20 through the experiment to assure high quality data. Off‐line the EEG was re‐referenced to the average reference, and band‐pass filtered from 0.1 to 30 Hz (24 dB) when ERPs were analyzed with Brain Vision Analyzer 2 (BVA2, Brain Products). Bipolar electromyograms (EMG) were recorded from the extensor digitorum muscle in the left and right forearms with Ag/AgCl electrodes using the BioSemi Active Two system and were off‐line high‐pass filtered with 5.31 Hz and full‐wave rectified with the BVA2. EMG was collected in order to identify covert movements whether of the correct hand or the incorrect hand. All physiological signals were digitized at a rate of 1,024 Hz.

Matsuhashi T., Segalowitz S.J., Murphy T.I., Nagano Y., Hirao T, & Masaki H. (2020). Medial frontal negativities predict performance improvements during motor sequence but not motor adaptation learning. Psychophysiology, 58(1), e13708.

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Comprehensive EEG Setup for Cognitive Studies

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EEG was recorded using an extended 10–20 montage with a 32-channel elastic stretch BioSemi headcap with the Active Two BioSemi system (BioSemi, Amsterdam, Netherlands). Two additional electrodes were placed on the left and right mastoids, and four additional facial electrodes were used to measure eye movement. Vertical eye movements were measured from electrodes placed on the infra-orbital ridges centered under the pupils of both eyes and corresponding supra-orbital electrodes embedded within the cap. Horizontal eye movements were measured from electrodes placed approximately 1 cm outside the participants’ right and left outer canthi. Data were recorded at 512 Hz with Actiview Software, v8.0.

Willner C.J., Jetha M.K., Segalowitz S.J, & Gatzke-Kopp L.M. (2019). Neurophysiological evidence for distinct biases in emotional face processing associated with internalizing and externalizing symptoms in children. Biological psychology, 150, 107829.

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EEG Recording with BioSemi System

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The EEG was recorded with an Active Two BioSemi system (BioSemi, Amsterdam, Netherlands) from 64 Ag/AgCl pin electrodes at standard 10–20 positions and two additional system-specific pin electrodes serving as ground (DRL) and internal reference (CMS) (positioned between POz and either PO3 and PO4, respectively). Electrodes were placed in an EEG-cap (Electro-Cap International, Eaton, OH, United States). Data were sampled at 512 Hz and hardware filtered with a 104 Hz low-pass filter. A Cedrus StimTracker (Cedrus Corporation, San Pedro, CA, United States) was used to detect and mark the onset of the visual stimuli.

Ströberg K., Andersen L.M, & Wiens S. (2017). Electrocortical N400 Effects of Semantic Satiation. Frontiers in Psychology, 8, 2117.

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EEG Data Collection and Preprocessing

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EEG data were collected from the scalp using either 33 channels or 64 channels from the same Active-Two BioSemi system (BioSemi, Inc., Amsterdam, the Netherlands) based on a modified 10-20 electrode placement system (American Electroencephalographic Society, 1994 (link)). Two electrodes, namely the common mode sense (CMS) and the driven right leg (DRL), were used to generate a common reference voltage (https://www.biosemi.com/faq/cms&drl.htm). Additional signals collected from the left and right earlobes were averaged and used for offline referencing. Two electrodes were placed at the supra- and infraorbital regions of the left eye to measure vertical eye movements. Two electrodes were placed at the left and right outer canthi to measure the horizontal eye movements. The sampling rate was 1024 Hz.

Lin M.H., Davies P.L., Stephens J, & Gavin W.J. (2020). Test-Retest Reliability of Electroencephalographic Measures of Performance Monitoring in Children and Adults. Developmental neuropsychology, 45(6), 341-366.

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Continuous EEG Recording and Startle Eye-Blink

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Continuous EEG activity was recorded using an ActiveTwo head cap and the ActiveTwo Biosemi system (BioSemi, Amsterdam, The Netherlands). Recordings were collected from 128 Ag-AgCl scalp electrodes and from bilateral mastoids. Two electrodes were placed next to each other 1 cm below the right eye to record startle eye-blink responses. A ground electrode was established by BioSemi’s common Mode Sense active electrode and Driven Right Leg passive electrode. EEG activity was digitized with ActiView software (BioSemi) and sampled at 2048 Hz. Data were downsampled post-acquisition and analyzed at 512 Hz.

Forbes C.E., Amey R., Magerman A.B., Duran K, & Liu M. (2018). Stereotype-based stressors facilitate emotional memory neural network connectivity and encoding of negative information to degrade math self-perceptions among women. Social Cognitive and Affective Neuroscience, 13(7), 719-740.

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Continuous EEG Acquisition: Detailed Methodology

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Continuous EEG was recorded during the task using an elastic cap and the ActiveTwo BioSemi system (BioSemi, Amsterdam, Netherlands) on the 10/20 system. Thirty‐four electrode sites were used (including FCz and Iz), along with one electrode placed on each mastoid. Electrooculogram (EOG) was recorded from four additional facial electrodes to measure eye blinks and eye movements: 1 cm above and below the right eye to measure vertical eye movements (VEOG) and 1 cm beyond each eye to measure horizontal eye movements (HEOG). The EEG signal was pre-amplified at the electrode to improve the signal-to-noise ratio. The data were digitized at 24-bit resolution with a least significant bit (LSB) value of 31.25 nV and a sampling rate of 1,024 Hz, using a low‐pass fifth order sinc filter with a −3dB cutoff point at 204.8 Hz.

Granros M., MacNamara A., Klumpp H., Phan K.L, & Burkhouse K.L. (2022). Neural Reactivity to Affective Stimuli and Internalizing Symptom Dimensions in a Transdiagnostic Patient Sample. Depression and anxiety, 39(12), 770-779.

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High-Density EEG Recording Protocol

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EEG data were recorded from 64 electrodes at standard 10–20 positions, and one electrode on the tip of the nose with an Active Two BioSemi system (BioSemi, Amsterdam, Netherlands). Although 64 electrodes are sufficient to capture electrical brain activity across the head, the nose electrode was included to allow for secondary analyses with only a minimal set of electrodes and the nose as the reference (these analyses are not discussed here). An EEG cap (Electro-Cap International, Eaton, OH) was used to position the 64 electrodes together with two additional, system-specific electrodes. CMS (between PO3 and POz) served as the internal reference electrode, and DRL (between POz and PO4) as the ground electrode. These 66 positions were recorded with pin electrodes, and the tip of the nose was recorded with a flat electrode attached with an adhesive disk. Data were sampled at 512 Hz and filtered with a hardware low-pass filter at 104 Hz.

Eklund R, & Wiens S. (2018). Visual awareness negativity is an early neural correlate of awareness: A preregistered study with two Gabor sizes. Cognitive, Affective & Behavioral Neuroscience, 18(1), 176-188.

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EEG Experiment on Benq Display

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Pictures were shown on a 24″ Benq XL2430-B TN-screen with a refresh rate of 100 Hz and a resolution of 1920 × 1080 pixels. Picture size was 9 cm (8.6°) wide and 11.3 cm (10.8°) high. The background was dark gray, viewing distance was 60 cm (maintained with a chin rest), and the experimental software was Presentation 17.0 (Neurobehavioral Systems, Albany, CA, United States).
The EEG apparatus was an Active Two Biosemi system (Biosemi, Amsterdam, Netherlands) with 128 active electrodes. Data were sampled at 512 Hz and filtered with a hardware low-pass filter at 104 Hz.

Syrjänen E., Wiens S., Fischer H., Zakrzewska M., Wartel A., Larsson M, & Olofsson J.K. (2018). Background Odors Modulate N170 ERP Component and Perception of Emotional Facial Stimuli. Frontiers in Psychology, 9, 1000.

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Continuous EEG Recording During Task

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Continuous EEG recording during the task was completed using an elastic cap and the ActiveTwo BioSemi system (BioSemi, Amsterdam, The Netherlands). Thirty-four electrode sites were used, based on the 10/20 system (standard 32 channel montage, as well as FCz and Iz). The voltage from each active electrode was referenced online with respect to a common mode sense (CMS) active electrode, producing a monopolar (non-differential) channel. Two electrodes were placed on the left and right mastoids, while an additional four facial electrodes were used to record the electrooculogram (EOG) generated from eye blinks and eye movements: two of these electrodes were located approximately one cm outside the outer edge of the right and left eyes and two electrodes were placed approximately one cm above and below the left eye. The EEG and EOG were low-pass filtered using a fifth order sinc filter with a half-power cutoff of 204.8 Hz and then digitized at 1024 Hz with 24 bits of resolution.

Fitzgerald J.M., MacNamara A., DiGangi J.A., Kennedy A.E., Rabinak C.A., Patwell R., Greenstein J.E., Proescher E., Rauch S.A., Hajcak G, & Phan K.L. (2015). An Electrocortical Investigation of Voluntary Emotion Regulation in Combat-Related Posttraumatic Stress Disorder. Psychiatry research, 249, 113-121.

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