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Anti cc3 antibody

Manufactured by Cell Signaling Technology
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The Anti-CC3 antibody is a laboratory reagent used to detect the presence of cleaved Caspase-3, a key executioner protein involved in apoptosis, or programmed cell death. This antibody specifically recognizes the cleaved form of Caspase-3, providing a reliable tool for researchers studying cell signaling and apoptotic pathways.

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Lab products found in correlation

Anti γ h2ax antibody, by Novus Biologicals (1 mentions) Anti ki67 antibody, by Roche (1 mentions) Trueview, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Anti-CC3 (14 citations) Rabbit anti-CC3 antibody (3 citations) Rabbit monoclonal anti-CC3 antibody (2 citations)

3 protocols using anti cc3 antibody

1

Immunofluorescence Microscopy Protocol

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Tubulin-specific mAb DM1α (Sigma-Aldrich), anti-centromere antibody (CREST), anti-CC-3 antibody (Cell Signaling), anti-Ki67-antibody (Ventana), anti-γ-H2AX-antibody (Novus Biologicals), GFP-specific antibody (William Wickner). Antibodies were used at dilutions of 1:1,000 or 1:10,000 (for GFP-specific antibody).
Bakhoum S.F., Kabeche L., Wood M.D., Laucius C.D., Qu D., Laughney A.M., Reynolds G.E., Louie R.J., Phillips J., Chan D.A., Zaki B.I., Murnane J.P., Petritsch C, & Compton D.A. (2015). Numerical chromosomal instability mediates susceptibility to radiation treatment. Nature communications, 6, 5990.
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2

Quantifying Apoptosis in Embryonic Liver

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Immunofluorescence for CC3 and TER-119 was performed manually on paraffin sections of embryos. After heat-induced epitope retrieval in a pH 6.0 citrate buffer, the primary antibodies were applied (rabbit polyclonal anti-CC3 antibody, Cell Signaling Technology, 9661, rat monoclonal anti–TER-119 antibody, BD Pharmingen, 550565, both at 1:200 dilution), followed by the application of secondary antibodies (anti-rabbit IgG conjugated with Alexa Fluor 488, Thermo Fisher, A-21206, anti-rat IgG conjugated with Alexa Fluor 594, Thermo Fisher, A-21209, both at 1:500 dilution), DAPI, and an autofluorescence quenching kit (TrueVIEW, Vector Laboratories, SP-8400-15). Slides were imaged on a BX-61 microscope, and CC3-positive cell quantification on whole liver sections was performed in Qupath 0.3.0.
Chen S., Vedula R.S., Cuevas-Navarro A., Lu B., Hogg S.J., Wang E., Benbarche S., Knorr K., Kim W.J., Stanley R.F., Cho H., Erickson C., Singer M., Cui D., Tittley S., Durham B.H., Pavletich T.S., Fiala E., Walsh M.F., Inoue D., Monette S., Taylor J., Rosen N., McCormick F., Lindsley R.C., Castel P, & Abdel-Wahab O. (2022). Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic Transformation. Cancer Discovery, 12(10), 2434-2453.
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3

Detect Cleaved Caspase-3 in Cells

Cited 1 time
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Cells were triturated into single cells, washed with phosphate buffered saline (PBS), fixed in cytospin fluid, and cytospun onto Plus slides (Fisher). Cells were then processed for cleaved caspase 3 (CC3) detection, using an anti-CC3 antibody (Cell Signaling Technology, Beverly, MA) as described (33 (link)).
Taylor I.C., Hütt-Cabezas M., Brandt W.D., Kambhampati M., Nazarian J., Chang H.T., Warren K.E., Eberhart C.G, & Raabe E.H. (2015). Disrupting NOTCH Slows Diffuse Intrinsic Pontine Glioma Growth, Enhances Radiation Sensitivity, and Shows Combinatorial Efficacy with Bromodomain Inhibition. Journal of neuropathology and experimental neurology, 74(8), 778-790.
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