Pe conjugated monoclonal mouse anti human cd133 1 ac133

Manufactured by Miltenyi Biotec

The PE-conjugated monoclonal mouse anti-human CD133/1 (AC133) is a flow cytometry reagent. It is used for the detection and analysis of CD133/1 (AC133) antigen expression on human cells.

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Lab products found in correlation

Phase microscope, by Olympus (1 mentions) Cellquest software, by BD (1 mentions) Facscalibur, by BD (1 mentions)

2 protocols using pe conjugated monoclonal mouse anti human cd133 1 ac133

Comprehensive Analysis of Cancer Cell Properties

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Total cell number counting, BrdU incorporation, cell cycle analysis, TUNEL, Annexin V, foci formation, soft agar colony formation assay, 3D-matrigel culture, in vitro cell motility (migration, invasion and wound healing) assays and tumorsphere formation assay were carried out as previously described [55 (link)–57 (link)]. All the images were taken using a phase microscope (Olympus, Tokyo, Japan). The CD133 positive population in HCC cells was examined by using PE-conjugated monoclonal mouse anti-human CD133/1 (AC133, Miltenyi Biotec; Auburn CA).

Zhang M., Zhang W., Wu Z., Liu S., Sun L., Zhong Y., Zhang X., Kong X., Qian P., Zhang H., Lobie P.E, & Zhu T. (2015). Artemin is hypoxia responsive and promotes oncogenicity and increased tumor initiating capacity in hepatocellular carcinoma. Oncotarget, 7(3), 3267-3282.

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CD133 Expression Profiling in Liver Cells

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Flow cytometry for CD133 expression was performed on liver cell lines using PE-conjugated monoclonal mouse anti-human CD133/1 (AC133, Miltenyi Biotec). Isotype control mouse IgG1-PE (eBioscience, San Diego, CA) served as a control. Samples were analyzed on a BD FACSCalibur (BD Biosciences, Franklin Lakes, NJ) and data were analyzed using CellQuest software (BD Biosciences).

Chai S., Tong M., Ng K.Y., Kwan P.S., Chan Y.P., Fung T.M., Lee T.K., Wong N., Xie D., Yuan Y.F., Guan X.Y, & Ma S. (2014). Regulatory role of miR-142-3p on the functional hepatic cancer stem cell marker CD133. Oncotarget, 5(14), 5725-5735.

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