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Mitofilin myc

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Mitofilin-myc is a protein that is found in the mitochondria of cells. It plays a role in maintaining the structure and function of the mitochondria.

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Lab products found in correlation

Slc25a46, by OriGene (2 mentions) Pcdna3, by Thermo Fisher Scientific (2 mentions)

2 protocols using mitofilin myc

1

Generating Fluorescent Protein Constructs

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HB9:Gal4-VP16 was generated from the pmT hb9s YFP kindly provided by the Nonet Lab at Wash U. UAS-E1b:mYFP,mitoCFP was kindly provided by Bettina Schmid36 (link) from the German Center for Neurodegenerative Diseases (DZNE). SLC25A46 and mitofilin-myc were obtained from (Origene). SLC25A46 was subcloned into pcDNA3.1 (Invitrogen) with either C-terminal HA or myc. For RNA synthesis SLC25A46-HA was also subcloned into PCS2+.
Abrams A.J., Hufnagel R.B., Rebelo A., Zanna C., Patel N., Gonzalez M.A., Campeanu I.J., Griffin L.B., Groenewald S., Strickland A.V., Tao F., Speziani F., Abreu L., Schüle R., Caporali L., La Morgia C., Maresca A., Liguori R., Lodi R., Ahmed Z.M., Sund K.L., Wang X., Krueger L.A., Peng Y., Prada C.E., Prows C.A., Bove K., Schorry E.K., Antonellis A., Zimmerman H.H., Abdul-Rahman O.A., Yang Y., Downes S.M., Prince J., Fontanesi F., Barrientos A., Nemeth A.H., Carelli V., Huang T., Zuchner S, & Dallman J.E. (2015). Mutations in the UGO1-like protein SLC25A46 cause an optic atrophy spectrum disorder. Nature genetics, 47(8), 926-932.
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2

Generating Fluorescent Protein Constructs

Check if the same lab product or an alternative is used in the 5 most similar protocols
HB9:Gal4-VP16 was generated from the pmT hb9s YFP kindly provided by the Nonet Lab at Wash U. UAS-E1b:mYFP,mitoCFP was kindly provided by Bettina Schmid36 (link) from the German Center for Neurodegenerative Diseases (DZNE). SLC25A46 and mitofilin-myc were obtained from (Origene). SLC25A46 was subcloned into pcDNA3.1 (Invitrogen) with either C-terminal HA or myc. For RNA synthesis SLC25A46-HA was also subcloned into PCS2+.
Abrams A.J., Hufnagel R.B., Rebelo A., Zanna C., Patel N., Gonzalez M.A., Campeanu I.J., Griffin L.B., Groenewald S., Strickland A.V., Tao F., Speziani F., Abreu L., Schüle R., Caporali L., La Morgia C., Maresca A., Liguori R., Lodi R., Ahmed Z.M., Sund K.L., Wang X., Krueger L.A., Peng Y., Prada C.E., Prows C.A., Bove K., Schorry E.K., Antonellis A., Zimmerman H.H., Abdul-Rahman O.A., Yang Y., Downes S.M., Prince J., Fontanesi F., Barrientos A., Nemeth A.H., Carelli V., Huang T., Zuchner S, & Dallman J.E. (2015). Mutations in the UGO1-like protein SLC25A46 cause an optic atrophy spectrum disorder. Nature genetics, 47(8), 926-932.
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