AFM imaging was performed using a MFP-3D microscope (Asylum Research, Goleta, CA, USA) in a tapping mode with a typical scan rate of 0.5 Hz. Images were taken in air using sharpened silicon cantilevers, SSS-SEIHR (Nanosensors, Neuchâtel, Switzerland), with guaranteed tip radius < 5 nm or standard cantilevers, OMCL-AC200TS (Olympus, Tokyo, Japan), with a typical tip radius of 7 nm. FemtoScan Online software (http://www.femtoscanonline.com ) was used to filter, analyze and present the AFM images. SPM Image Magic software (https://sites.google.com/site/spmimagemagic ) was used for a semi-automatic measurement of the height of visualized objects.
Omcl ac200ts
Manufactured by Olympus
Sourced in Japan
The OMCL-AC200TS is a laboratory centrifuge designed to separate samples based on their density. It features a maximum speed of 20,000 RPM and can accommodate a variety of sample containers. The centrifuge is capable of generating a maximum relative centrifugal force (RCF) of 48,400 x g.
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Lab products found in correlation
2 protocols using omcl ac200ts
1
Atomic Force Microscopy Imaging Protocol
2
Peptide Characterization by AFM
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For microscopy studies, peptide solutions at a concentration of 5 × 10−4 M were prepared at the desired pH values. AFM samples were prepared by drop-casting 10 μL of the respective sample onto a mica surface, followed by spin-coating at 1000 rpm, and then, the thin film was dried at room temperature for 24 h. After that, images were captured on a Multimode® 8 SPM System (AXS Bruker). Silicon cantilevers with a nominal spring constant of 9 Nm−1 and with a resonant frequency of ≈150 kHz and a typical tip radius of 7 nm (OMCL-AC200TS, Olympus) were employed.
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