Pl 21

Human AML cell lines THP-1, MV4-11, OCI-AML-3, PL-21, MOLM13, U937, NALM-6 were purchased from ATCC (USA). All cell lines were cultured in RPMI containing 20% FBS, 2 mM L-Glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. Murine J774A.1 cell line was provided by Peter Düwell, Institute of Innate Immunity, University Hospital, Bonn. Cells were cultured in DMEM containing 10 % FBS, 2 mM L-Glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. All cells were grown at 37°C in a humidified incubator with 5% CO₂. Short tandem repeat (STR) profiling was used to verify identity of human cell lines. Cells were negatively tested for mycoplasma contamination using polymerase chain reaction (PCR). All cell lines were lentivirally transduced with a pCDH-EF1a-eFly-eGFP plasmid^{85 (link)}. After transduction, enhanced green-fluorescent protein (eGFP) positive cells were single-cell sorted using a BD FACSAria™ III Cell Sorter and expression of firefly luciferase (fLuc) was verified using Bio-Glo™ Luciferase Assay System. Cells were frozen in medium containing 90% FCS and 10% DMSO and stored at -80°C or in liquid nitrogen for long-term storage. Generation of PDX cells was previously described^{48 (link)}. Anti-mouse c-fms (CD115)-producing Hybridoma RCB4486 was acquired from the RIKEN Bio-Resource Research Center^{86 (link)}.

PL-21, THP-1, MOLM-13, MV4-11, E.G7-OVA and SEM cell lines were purchased from ATCC (USA). The E.G7-OVA cell line was modified to express full-length human EGFRvIII (Uniprot Entry P00533 AA 1-29, 298-646), resulting in E.G7-EGFRvIII cells. Luciferase-eGFP (LUC-GFP) overexpressing cell lines PL-21-LUC-GFP, THP-1-LUC-GFP and MV4-11-LUC-GFP were generated according to previously described protocols (22 (link)). Antigen quantification of cell lines are summarized in Supplementary Table 1A. 293Vec-Galv and 293Vec-RD114 were a kind gift of Manuel Caruso, Québec, Canada and have been previously described (27 (link)). All human cell lines were short tandem repeat profiled in house to verify their origin. Cells were used for a time period no longer than two months.