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Timebase software

Manufactured by PerkinElmer
Sourced in United Kingdom

TimeBase software is a data analysis and reporting tool developed by PerkinElmer. It provides a comprehensive platform for managing and analyzing data from various laboratory instruments and experiments.

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4 protocols using timebase software

1

Measuring Polymer Diffusion Curves

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Diffusion curves
in polymer films were
measured of cyclohexane (904 cm–1), ethanol (879
cm–1), acetone (529 cm–1), D2O (2510 cm–1), methanol-d4 (2485 cm–1), DCM (1265 cm–1), and toluene-d8 (541 cm–1). The wavenumbers in parentheses for each solvent refer to the positions
of a characteristic solvent band that was sufficiently isolated for
accurate band integration. The time-dependent band areas were calculated
with PerkinElmer TimeBase software using a baseline anchored to the
spectrum to either side of a band and required no further processing
before model fitting. FTIR spectra of Znpol at maximum solvent swelling
for all solvents are shown in Figure S2. The reproducibility of the diffusion curves was investigated by
repeating the acetone diffusion experiment eight times on Znpol films
with thicknesses varying between 124 and 159 μm. The standard
deviation in the thickness measurement, caused by uneven sample surfaces,
was ∼10%. The standard deviation in the swelling factor was
determined by repeating the experiment for acetone nine times and
was found to be 0.13. We have applied a method to calculate the 95%
confidence intervals of the fitting parameters using a least-squares
procedure43 (link) (see the Supporting Information). The 95% confidence interval in DFick resulting from the parameter estimation
procedure was below 4%, whereas for Deq the interval was between 27% and 34%.
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2

Monitoring Polymerization of Self-Etch Adhesives

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The polymerization processes of model self-etch adhesives were monitored by using a Fourier transformed infrared spectrometer equipped with an attenuated total reflectance (ATR) attachment (Spectrum One, PerkinElmer, Waltham, MA, USA). Each DHEPT or HAp- containing adhesive was vortexed for 1 min to facilitate DHEPT/HAp dissolution, and then one drop of adhesive was placed on the ATR top-plate, subsequently covered by a plastic cover slip. Programmed collection of spectra started instantly using TimeBase software (Perkin-Elmer). Continuous spectral collection was carried-out with up to 0.4- to 0.6-s spectral collection intervals depending on collection speed. For the light-cure only and dual-cure groups, after collecting the initial ~50 spectra, light irradiation began and lasted for 10, or 30 s. Conventional dental light (Spectrum Light; Dentsply, Milford, DE, USA) was used with output power at 600 mW/cm2, and the curing tip was purposely set at a long distance (1 cm) away from the cover slip. Otherwise, all adhesive groups were shielded from the ambient light throughout spectral collection (up to 60 h). The spectra were recorded at a resolution of 4 cm−1 in the wave-number range between 700 and 4000 cm−1. Due to high spectral quality, baseline correction was not needed.
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3

FTIR Analysis of Glass Compositions

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The infrared band assignment of each glass composition was determined by FTIR (FT-IR 2000 and Timebase software, Perkin Elmer, Seer Green, UK). Specimens of glass powder were placed on an attenuated total reflectance accessory (Golden Gate, Specac, Orpington, UK) and spectra in the range of 2000–600 cm−1 were acquired at room temperature in absorbance mode.
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4

Monitoring pH-Induced Ion-Pair Dissociation in Theophylline-Spermine Formulations

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To simulate ion-pair dissociation induced by a pH change when introduced into the vascular perfusate, theophylline-spermine formulations were made up in D2O and in PG-D2O at pH 9.6 ± 0.2 at a theophylline concentration of 3.6 mg.mL -1 and a spermine concentration of 83 mg.mL -1 . Just prior to measurement, the pH of the test solutions was adjusted to pH 7.4 ± 0.2 using a pre-determined volume of HCl (5 M). The samples were then mounted in a universal transmission cell system (Omni-Cell, Specac Ltd., UK) fitted with CaF2 windows and a 25 µm Mylar spacer (Specac Ltd., UK) mounted on a Spectrum One spectrometer (Perkin Elmer Ltd., UK). A total of 46 s elapsed before the first scan was taken (time needed to mount the cell onto the spectrometer) and the continuous monitoring of the C-N7 and C6=O was carried out for 20 min using TimeBase™ software (Perkin Elmer Ltd., UK). The pH was monitored throughout the process.
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