Fubon feed additive and S. boulardii β-glucan (purity 80%, molecular mass 800–1000 kDa) were purchased from Angel Yeast Co., Ltd. (Yichang, China). Yeast Extract Peptone Dextrose Medium (YPD) was purchased from Qingdao Hi-Tech Park Haibo Biotechnology. Dextran sulfate sodium (DSS, w/v. molecular mass 36–50 kDa) was purchased from Yisheng Biotechnology Co., Ltd. (Shanghai, China). RIPA buffer (high) and PMSF were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Protease inhibitor, ZO-1 Rabbit Polyclonal Antibody, Occludin Rabbit Polyclonal Antibody, GAPDH Rabbit Polyclonal Antibody, Horseradish peroxidase-conjugated goat anti-rabbit IgG(H + L) secondary antibody, and an ELISA Kit (Mouse TNF-α, IL-1β, and IL-6) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). TransZolTM Up Plus RNA Kit and Reverse transcriptase reagent kit were purchased from Beijing TransGen Biotechnology Co., Ltd. (Beijing, China). SuperReal PreMix Plus (SYBR Green) was purchased from Tiangen Biochemical Technology Co., Ltd. (Beijing, China).
Horseradish peroxidase conjugated goat anti rabbit igg h l secondary antibody
Manufactured by Beyotime
Sourced in China
Horseradish peroxidase-conjugated goat anti-rabbit IgG(H + L) secondary antibody is a laboratory reagent used to detect and quantify the presence of rabbit primary antibodies in various experimental techniques. The antibody is conjugated with the enzyme horseradish peroxidase, which can catalyze a colorimetric or luminescent reaction, allowing for the visualization and quantification of target proteins.
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Lab products found in correlation
Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions)
Ripa buffer high, by Solarbio (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Anti osteocalcin ocn, by Abcam (1 mentions)
Image pro plus software 6, by Media Cybernetics (1 mentions)
Hematoxylin, by Beyotime (1 mentions)
2 protocols using horseradish peroxidase conjugated goat anti rabbit igg h l secondary antibody
1
Probiotic and β-Glucan Modulate Colitis
2
Decalcification, Paraffin Embedding, and Immunohistochemical Analysis
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Following micro-CT testing, the specimens were decalcified with 10% EDTA, dehydrated, paraffin embedded, sectioned into 5-µm-thick slices for hematoxylin and eosin (HE; 30 min at room temperature) and Masson trichrome (20 min at room temperature) staining, and examined by light microscopy.
For immunohistochemistry, the sections were blocked with 5% bovine serum albumin (Invitrogen; Thermo Fisher Scientific, Inc.) for 30 min at room temperature and then incubated with the anti-osteocalcin (OCN; Abcam, Cambridge, UK; cat. no. ab198228; 1:200) primary antibody overnight at room temperature. The sections were then incubated with the horseradish peroxidase-conjugated goat anti-rabbit IgG (H+L) secondary antibody (Beyotime Institute of Biotechnology; cat. no. A0208) at a dilution of 1:200 for 1 h at room temperature, visualized with 3,3-diaminobenzidine solution, and counter-stained with hematoxylin (Beyotime Institute of Biotechnology) for 1 h at room temperature. Image Pro Plus software 6.0 (Media Cybernetics, Inc., Rockville, MD, USA) was used to analyze the results.
For immunohistochemistry, the sections were blocked with 5% bovine serum albumin (Invitrogen; Thermo Fisher Scientific, Inc.) for 30 min at room temperature and then incubated with the anti-osteocalcin (OCN; Abcam, Cambridge, UK; cat. no. ab198228; 1:200) primary antibody overnight at room temperature. The sections were then incubated with the horseradish peroxidase-conjugated goat anti-rabbit IgG (H+L) secondary antibody (Beyotime Institute of Biotechnology; cat. no. A0208) at a dilution of 1:200 for 1 h at room temperature, visualized with 3,3-diaminobenzidine solution, and counter-stained with hematoxylin (Beyotime Institute of Biotechnology) for 1 h at room temperature. Image Pro Plus software 6.0 (Media Cybernetics, Inc., Rockville, MD, USA) was used to analyze the results.
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