Anti-HA and anti-FLAG primary antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-PARP1 primary antibody was purchased from Abcam (Cambridge, UK). Immunoaffinity-purified rabbit polyclonal antibodies against PD-L1, LMNB1, and GAPDH were obtained from Saier Biotech (Tianjin, China). Horseradish peroxidase-conjugated anti-mouse and anti-rabbit secondary antibodies were purchased from Zhongshan Golden Bridge Biotechnology (Beijing, China). The FITC/TRITC-conjugated secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). FITC-conjugated CD69 (human/mouse), APC-conjugated TNF-α (human/mouse), Brilliant Ultraviolet 615-conjugated IFN-γ (human/mouse), PE-Cy7-conjugated CD3 (mouse), PE-conjugated CD8 (mouse), APC-conjugated CD4 (mouse), and FITC-conjugated CD4 (mouse) were purchased from eBioscience (San Diego, CA, USA). Anti-PD-L1 (PE-conjugated CD274, human) was purchased from BD Biosciences (NJ, CA, USA).
Con A was obtained from Solarbio (Beijing, China), and actinomycin D (Act D), cycloheximide (CHX), and puromycin were purchased from Sigma-Aldrich. CellTrace CFSE was purchased from Invitrogen (Carlsbad, CA, USA). The compounds were dissolved in DMSO and used at the indicated concentrations.
Con A was obtained from Solarbio (Beijing, China), and actinomycin D (Act D), cycloheximide (CHX), and puromycin were purchased from Sigma-Aldrich. CellTrace CFSE was purchased from Invitrogen (Carlsbad, CA, USA). The compounds were dissolved in DMSO and used at the indicated concentrations.