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H 2 pe

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H-2- PE is a fluorescent dye that binds to the H-2 complex, a major histocompatibility complex found in mice. It is used for the detection and analysis of H-2 expression in flow cytometry applications.

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Lab products found in correlation

Attune nxt acoustic focusing cytometer, by Thermo Fisher Scientific (1 mentions) Cd45 apc, by BioLegend (1 mentions) Ifn γ pe, by BioLegend (1 mentions) Pd l1 apc, by BioLegend (1 mentions) Cd11b pe cy7, by BioLegend (1 mentions) Nk1.1 apc, by BioLegend (1 mentions) Nkp46 apc, by BioLegend (1 mentions) Cd3 af488, by BioLegend (1 mentions) Zombie violet, by BioLegend (1 mentions) Cd45 percp cy5, by BioLegend (1 mentions) I a 1 e af700, by BioLegend (1 mentions) H2 dd pe, by BioLegend (1 mentions) Nkg2a pe, by Miltenyi Biotec (1 mentions) Cd107b fitc, by BioLegend (1 mentions)

2 protocols using h 2 pe

1

Multiparameter Flow Cytometry Analysis

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Samples were run on an Attune NxT Acoustic Focusing Cytometer (Life Technologies). Analysis was performed in FlowJo. Gating was first done on forward scatter and side scatter to exclude debris. Doublets were excluded by gating on FSC area versus FSC height (Supplementary Fig. S8) Zombie Violet (BioLegend) was used to exclude dead cells from analyses. Antibodies: CD3-AF488 (BioLegend, clone HIT3a), Cd8a-AF488 (BioLegend, clone 53*6.7), CD45-PerCP/Cy5.5 (BioLegend, clone GK1.5), CD45-APC (BioLegend, clone 30-F11), NK1.1-APC (BioLegend, clone PK136), NKp46- APC (BioLegend, clone 29A1.4), CD11b- PE/Cy7 (BioLegend, clone M1/70), CD11c-AF488 (BioLegend, N418), IA/IE-AF700 (BioLegend M5/114.15.2), CD27 (BioLegend, clone LG.3A10), H-2- PE (BioLegend, clone M1/42), NKG2A-PE (Miltenyi Biotec, clone REA1161), H2-Dd-PE (BioLegend, clone 34-2-12), Qa-1b(b)-PE (BD Biosciences, clone 6A8.6F10.1A6), PD-L1–APC (BioLegend, clone 29E.2A3), CD49b-APC/Cy7 (BioLegend, clone DX5), and CD107b-FITC (BioLegend, clone 1D4B), IFNγ-PE (BioLegend, clone XMG1.2).
Taylor B.C., Sun X., Gonzalez-Ericsson P.I., Sanchez V., Sanders M.E., Wescott E.C., Opalenik S.R., Hanna A., Chou S.T., Van Kaer L., Gomez H., Isaacs C., Ballinger T.J., Santa-Maria C.A., Shah P.D., Dees E.C., Lehmann B.D., Abramson V.G., Pietenpol J.A, & Balko J.M. (2023). NKG2A Is a Therapeutic Vulnerability in Immunotherapy Resistant MHC-I Heterogeneous Triple-Negative Breast Cancer. Cancer Discovery, 14(2), 290-307.
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2

Multi-parameter Immune Cell Analysis

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Anti-mouse CD4 (BV421, PE, APC, and Percp-Cy5.5), CD8 (FITC, APC, and PE-Cy7), Ly6d (FITC), CD2 (PE and APC), CD69 (PE), Ki67 (BV421), CD3e (BV421), and H2 (PE) antibodies were purchased from BioLegend. Anti-mouse RORγt (PE) antibodies were obtained from BD Bioscience. All fluorescent antibodies were used according to the user manuals.
Li Y., Li K., Zhu L., Li B., Zong D., Cai P., Jiang C., Du P., Lin J, & Qu K. (2021). Development of double-positive thymocytes at single-cell resolution. Genome Medicine, 13, 49.
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