Freestyle optium β ketone

Blood samples were obtained from the tail vein (seven animals per group) and D-BHB was measured using blood glucose and ketone monitoring system (FreeStyle Optium Neo, Abbott Diabetes Care, Limited, Witney, Oxon, UK) and keto strips (FreeStyle Optium β-ketone). Samples were obtained from intact control and fasted animals at different times throughout the experimental period. D-BHB was also determined in blood samples from animals of the Coma and the Coma + BHB groups, before (time 0) and at different times after insulin injection (1 and 2 h), at the time the animals reached the coma, at different times after recovery with glucose or glucose + D-BHB (250 mg/kg), and after the second administration of D-BHB (250 mg/kg), as indicated in Figure 2C. Animals from these groups were identically treated during the hypoglycemia period before recovery and were randomly assigned to each one of the treatments.

D-BHB and glucose were determined from blood samples taken from the tail vein (eight animals per group) using the blood glucose and ketone monitoring system (FreeStyle Optium Neo, Abbott Diabetes Care, Limited, Witney, Oxon, UK) and keto strips (FreeStyle Optium β-ketone). Data were collected from control diet and KD-fed animals before the onset of treatments and 2 weeks later when treatments were concluded.