Spanish cocoa butter was purchased from Sunin Co. (Yongin, Korea). Palm stearin and shea butter were obtained from Ottogi Co. (Anyang, Korea) and Korea Similac (Pochun, Korea), respectively. HPLC-grade n-hexane (>99.5%), acetone (>99.3%), acetonitrile (>99.9%), and dichloromethane (>99.9%) were procured from J.T. Baker (Phillipsburg, NJ, USA). Silica gel–coated glass plates (20 cm × 20 cm, 0.25 mm thick) for thin-layer chromatography (TLC) were purchased from Merck KGaA (Darmstadt, Germany). Pancreatic lipase was acquired from Sigma Chemical Co. (St. Louis, MO, USA). Triolein (OOO; ≥99%), 1,2-dioleoyl-3-palmitoyl-rac-glycerol (POO; ≥99%), POP (≥99%), and PPP (≥99%), used as TAG standards, were also purchased from Sigma Chemical Co. All other reagents used herein were HPLC-grade.
1 2 dioleoyl 3 palmitoyl rac glycerol
Manufactured by Merck Group
1,2-dioleoyl-3-palmitoyl-rac-glycerol is a synthetic lipid compound used in various laboratory applications. It is a triglyceride molecule composed of two oleic acid chains and one palmitic acid chain. This product is commonly utilized in studies involving cell membranes, liposomes, and other lipid-based systems.
Automatically generated - may contain errors
Lab products found in correlation
Pancreatic lipase, by Merck Group (1 mentions)
Cytochrome c antibody, by Santa Cruz Biotechnology (1 mentions)
α tubulin, by Developmental Studies Hybridoma Bank (1 mentions)
1 3 dipalmitoyl 2 oleoylglycerol, by Merck Group (1 mentions)
1 palmitoyl 2 oleoyl 3 linoleoyl rac glycerol, by Merck Group (1 mentions)
1 3 dioleoyl 2 palmitoylglycerol, by Merck Group (1 mentions)
Glycerol reagent, by Merck Group (1 mentions)
Pnlip, by Santa Cruz Biotechnology (1 mentions)
Il 12, by Thermo Fisher Scientific (1 mentions)
Il 18, by R&D Systems (1 mentions)
4 protocols using 1 2 dioleoyl 3 palmitoyl rac glycerol
1
Lipid Analysis of Cocoa Butter Substitutes
2
Preparation and Characterization of TAG Drops
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A suspension (10 mg/ml) of TAG drops was prepared by dissolving 1 mg of 1,2-dioleoyl-3-palmitoyl-rac-glycerol (Sigma-Aldrich Inc.) in 100 μl of a chloroform-methanol solution (2:1). Ten microliters of the TAG drop suspension was pipetted on a custom-made dish (fig. S17; with a ZnS window as dish bottom) and left to dry at room temperature until the chloroform and methanol were completely evaporated, leaving the TAG drops on the surface of the ZnS window. After evaporation of chloroform and methanol, the dish was perfused with deionized water and covered with a cover glass, maintaining a 3-mm depth of water in the dish during the measurement. On the ZnS window, a reference region was maintained without any TAG drops for the RB.
3
Triglyceride and Lipid Metabolism Analysis
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Glyceryl trilinolein (GTL) and other triglycerides including triolein (GTO), tripalmitin (GTP), and mixed triglycerides 1,2-dilinoleoyl-3-palmitoyl-rac-glycerol (LLP), 1-palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol (LOP), 1,3-dipalmitoyl-2-linoleoylglycerol (PLP), 1,2-dioleoyl-3-palmitoyl-rac-glycerol (OOP), 1,3-dioleoyl-2-palmitoylglycerol (OPO), 1,3-dipalmitoyl-2-oleoylglycerol (POP), linoleic acid (LA), palmitic acid (PA), oleic acid (OA), dimethyl sulfoxide (DMSO), and glycerol reagent were purchased from Sigma-Aldrich (St. Louis, MO). CER (Bachem AG, Bubendorf, Switzerland), IL12 (PeproTech, Rocky Hill, NJ), IL18 (R&D Systems, Minneapolis, MN), PNLIP, IκB-α, cytochrome c antibody (Santa Cruz Biotechnology, Dallas, TX), α-tubulin [Developmental Studies Hybridoma Bank (DSHB), University of Iowa, Iowa], Cox-IV antibody, and TO-PRO-3 iodide (Thermo Fisher Scientific, Waltham, MA) were used.
4
Preparation and Characterization of TAG Drops
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Using a 10 mg/mL
solution of 1,2-dioleoyl-3-palmitoyl-rac-glycerol (Sigma-Aldrich Inc.),
TAG drops were prepared by dissolving 1 mg of this solution in 100
μL of a chloroform–methanol solution (2:1). 10 μL
of the TAG solution was placed on a custom-made Petri dish with a
ZnS window and left to dry at room temperature until complete evaporation
of chloroform–methanol, leaving the TAG drops on the surface
of the ZnS window. The TAG drop sample was then used for proof-of-concept
measurements as shown inFigure 1 . Water-immersed TAG drops (Figure 2 j–m) were prepared by adding a 1 mm
layer of deionized water and covered with cover glass (0.17 mm thick)
to reduce water evaporation and maintain the thickness of the water
layer. Optothermal signal characterization (Figure 2 b–i) was performed in a layer of 1
mm of water, detecting the signal from water between the ZnS and cover
glass.
solution of 1,2-dioleoyl-3-palmitoyl-rac-glycerol (Sigma-Aldrich Inc.),
TAG drops were prepared by dissolving 1 mg of this solution in 100
μL of a chloroform–methanol solution (2:1). 10 μL
of the TAG solution was placed on a custom-made Petri dish with a
ZnS window and left to dry at room temperature until complete evaporation
of chloroform–methanol, leaving the TAG drops on the surface
of the ZnS window. The TAG drop sample was then used for proof-of-concept
measurements as shown in
layer of deionized water and covered with cover glass (0.17 mm thick)
to reduce water evaporation and maintain the thickness of the water
layer. Optothermal signal characterization (
mm of water, detecting the signal from water between the ZnS and cover
glass.
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