Sb203580 5633

Manufactured by Cell Signaling Technology

Sourced in United States

SB203580 (5633) is a selective and potent inhibitor of p38 mitogen-activated protein kinase (MAPK). It functions by blocking the activation of MAPK-activated protein kinases, thereby inhibiting the p38 MAPK signaling pathway.

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Lab products found in correlation

Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Anti gapdh sc 32233, by Santa Cruz Biotechnology (1 mentions) Pci34051 10444, by Cayman Chemical (1 mentions) L ascorbic acid a7506, by Merck Group (1 mentions) Hrp conjugated anti rabbit igg 7074, by Cell Signaling Technology (1 mentions) Mouse anti gapdh nb300 221 antibody, by Novus Biologicals (1 mentions) Recombinant human tnfα 210 ta, by R&D Systems (1 mentions) U0126 9903, by Cell Signaling Technology (1 mentions) Si sp1, by Thermo Fisher Scientific (1 mentions) Si nc, by Thermo Fisher Scientific (1 mentions) Normal rabbit igg 2729, by Cell Signaling Technology (1 mentions) Siahr, by Thermo Fisher Scientific (1 mentions) Anti β actin ab8227, by Abcam (1 mentions) Pd98059 9900s, by Cell Signaling Technology (1 mentions) Siarnt, by Thermo Fisher Scientific (1 mentions) Streptavidin horseradish peroxidase hrp conjugate, by Merck Group (1 mentions) Phospho p38 mapk thr180 tyr182 antibody 9211, by Cell Signaling Technology (1 mentions) Ponceau s solution, by Merck Group (1 mentions) Chelerythrine chloride, by Merck Group (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

SB203580 (186 citations)

4 protocols using sb203580 5633

Cardiac Hypertrophy Signaling Pathway

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PCI34051 (10,444) was purchased from Cayman Chemical Company (An Arbor, MI, United States), and isoproterenol (isoprenaline hydrochloride, I5627) and l-ascorbic acid (A7506) were purchased from Sigma-Aldrich Co. (St. Louis, MO, United States). Anti-GAPDH (sc-32233) and anti-BNP (sc-271185) antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, United States). Anti-p38 MAPK (8690) antibody, anti-phospho-p38 MAPK (4511) antibody, and SB203580 (5633) were purchased from Cell Signaling Technology (Danvers, MA, United States). Anti-ANP antibody was purchased from GeneTex (GTX109255; Irvine, CA, United States). Alexa Fluor 488 phalloidin was purchased from Invitrogen (Eugene, OR, United States). Anti-HDAC8 (17548-1-AP) antibody was purchased from Proteintech Group (Rosemont, IL, United States).

Zhao T., Kee H.J., Bai L., Kim M.K., Kee S.J, & Jeong M.H. (2021). Selective HDAC8 Inhibition Attenuates Isoproterenol-Induced Cardiac Hypertrophy and Fibrosis via p38 MAPK Pathway. Frontiers in Pharmacology, 12, 677757.

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Signaling Pathways Activation in TNF-α and CCL21 Response

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Recombinant human TNF‐α (210‐TA) and CCL21 (366‐6C) were purchased from R&D Systems (Minneapolis, MN, USA). The following antibodies were used in Western blot analyses and immunocytochemistry: rabbit anti‐TNF‐α (ab6671), goat anti‐rabbit FITC‐conjugated IgG H&L (ab6717), and rabbit anti‐CCR7 (ab103404) from Abcam (Cambridge, MA, USA); rabbit anti‐p38 (8690), rabbit anti‐phospho‐p38 (Thr180/Tyr182; 4511), rabbit anti‐ERK (9102), rabbit anti‐phospho‐ERK (Thr202/Tyr204; 9101), and HRP‐conjugated anti‐rabbit IgG (7074) antibodies from Cell Signaling Technology (Danvers, MA, USA); and mouse anti‐GAPDH (NB300‐221) antibody from Novus Biologicals (Littleton, CO, USA). The ERK inhibitor U0126 (9903) and the p38 inhibitor SB203580 (5633) were purchased from Cell Signaling Technology.

Maolake A., Izumi K., Natsagdorj A., Iwamoto H., Kadomoto S., Makino T., Naito R., Shigehara K., Kadono Y., Hiratsuka K., Wufuer G., Nastiuk K.L, & Mizokami A. (2018). Tumor necrosis factor‐α induces prostate cancer cell migration in lymphatic metastasis through CCR7 upregulation. Cancer Science, 109(5), 1524-1531.

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Regulation of MMP2 and MMP11 Expression

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HaCaT keratinocytes and normal human dermal fibroblasts (NHDF) were a gift from Luis Garza (Johns Hopkins University). Anti–β-actin (ab8227) and anti-MMP2 (WB: ab97779; IHC: ab86607) antibodies were purchased from Abcam. Anti-MMP11 antibody (NBP2-67670) was purchased from Novus. Anti–phospho-SP1 (Thr-278: PA5-106039; Thr-739: PA5-104771), HRP-conjugated anti–rabbit IgG (A27036), HRP-conjugated anti–mouse IgG (A28177), and A488-conjugated anti-mouse (A11029) antibodies were purchased from Invitrogen. Anti-AhR (83200S), anti-ARNT (5537S), anti-SP1 (9389S), anti-ATM (2873T), anti-γH2AX (2577S), and normal rabbit IgG (2729S) antibodies were purchased from Cell Signaling Technologies. CH223191 (C8124), vitamin B₁₂ (V2876), and FA (F7876) were purchased from Sigma-Aldrich. PD98059 (9900S), SB203580 (5633S), and SP600125 (8177S) were purchased from Cell Signaling Technologies. The following siRNAs were purchased from Thermo Fisher: siNC (catalog 4390843), siAHR (catalog 4390824, s1199), siARNT (catalog 4392420, s1615), siSP1 (catalog 4392420, s13319), and siATM (catalog 4392420, s57221).

Kim D.J., Iwasaki A., Chien A.L, & Kang S. (2022). UVB-mediated DNA damage induces matrix metalloproteinases to promote photoaging in an AhR- and SP1-dependent manner. JCI Insight, 7(9), e156344.

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Amyloid-β Peptide Binding and Signaling

Cited 6 times

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Most of the material used in this study was purchased as we reported earlier [4 (link),53 (link),68 (link),96 (link),97 (link),98 (link),99 (link),100 (link)]. Aβ 1–40 (AS-24235), Aβ 1–42 (AS-20276), Aβ 1–28 (AS-24231), Aβ 25–35 (AS-24227), and Aβ 1–16 (AS-24225) were purchased from AnaSpec. Biotin-HN (HN, B-018--26, UniProt Q8IVG9) was purchased from Phoenix Pharmaceuticals. Phosphate Buffered Saline (PBS), nitrocellulose membranes, streptavidin–horseradish peroxidase (HRP) conjugate, Ponceau S solution, chelerythrine chloride, and ACh were purchased from Sigma-Aldrich. Mouse α-tubulin monoclonal antibody (DM1A), goat anti-rabbit IgG (H+L) secondary antibody (HRP, 31466), 3,3′,5,5′-tetramethylbenzidine (TMB), BCA protein assay kit, super signal west pico luminol (chemiluminescence) reagent, lipofectamine 2000 transfection reagent, and the Halt protease and phosphatase inhibitor cocktail were from ThermoFisher. Donkey anti-mouse IgG (HRP) (ab205724) was purchased from Abcam. m-IgGκ BP-HRP was obtained from Santa Cruz Biotechnology. SignalSilence p53 siRNA I (6231), SignalSilence control siRNA (Unconjugated, 6568), rabbit p53 antibody (9282), p38 MAPK antibody (9212) that detects endogenous levels of total p38α/β/γ MAPK, phospho-p38 MAPK (Thr180/Tyr182) antibody (9211), PKCα antibody (2056), and SB203580 (5633S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Al Khashali H., Ray R., Darweesh B., Wozniak C., Haddad B., Goel S., Seidu I., Khalil J., Lopo B., Murshed N., Guthrie J., Heyl D, & Evans H.G. (2024). Amyloid Beta Leads to Decreased Acetylcholine Levels and Non-Small Cell Lung Cancer Cell Survival via a Mechanism That Involves p38 Mitogen-Activated Protein Kinase and Protein Kinase C in a p53-Dependent and -Independent Manner. International Journal of Molecular Sciences, 25(9), 5033.

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