Myd88 23230 1 ap

Recombinant murine IL-1b (#211-11B) was purchased from PeproTech (Rocky Hill, NJ, USA). ZVAD was purchased from Selleck Chemicals (Houston, TX, USA). A mouse TNF-α ELISA kit was purchased from Bangyi (Shanghai, China). Murine anti-GAPDH antibody (BM3876) and secondary antibodies were purchased from Boster (Wuhan, China). Antibodies against RIPK1 (#3493), p-RIPK1 (83613), p-JNK (#9255), p-IkBa (#2859), p-P65 (#3033), TRAF2 (#4712), cleaved PARP (#9544), and cleaved caspase-3 (#9964) were purchased from Cell Signaling Technology (Beverly, MA, USA). Rabbit antibodies against JNK (24164-1-AP), IkBa (10268-1-AP), p65 (10745-1-AP), matrix metalloproteinase (MMP) 1 (10371-2-AP), and Myd88 (23230-1-AP) were purchased from Proteintech Group (Wuhan, Hubei, China). Antibodies against p-MLKL (ab196436), TRIF (180619), MMP3 (ab53015), and MMP13 (ab39012) were obtained from Abcam (Cambridge, UK). Pam3CSK4 and Poly (I:C) were obtained from Tocris Bioscience (Bristol, UK). A terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) apoptosis detection kit was obtained from Beyotime (Shanghai, China).

LPS was obtained from Solarbio (055: B5, Beijing, China). The antibodies against MyD88 (23230-1-AP), NF-κB p65 (10745-1-AP), and phospho-NF-κB p65 were obtained from Proteintech (Hubei, China). The antibodies against TRL4 (GB11519) and β-actin were gained from Servicebio (Hubei, China). All secondary antibodies used for western blot were purchased from ImmunoWay (Plano, TX). Cy3-conjugated goat anti-rabbit IgG (H + L) used in immunofluorescence experiments was obtained from Servicebio (Hubei, China).