The constructs pBIN35S-p22 and pETDuet.SAHH have been previously described (Cañizares et al., 2008 (link), Cañizares et al., 2013 (link)). The 35S-dsSAHH construct was kindly provided by Dr. David M. Bisaro (The Ohio State University, Columbus, OH, USA), and plasmids 35S-GFP and 35S-p19 were kindly provided by Dr. David C. Baulcombe (University of Cambridge, Cambridge, United Kingdom).
Pet28a
The PET28a is a plasmid vector used for the expression of recombinant proteins in Escherichia coli. It contains a T7 promoter and a His-tag sequence for efficient purification of the expressed proteins.
Lab products found in correlation
3 protocols using pet28a
Cloning and Mutagenesis of Tomato Chlorosis Virus p22 Gene
The constructs pBIN35S-p22 and pETDuet.SAHH have been previously described (Cañizares et al., 2008 (link), Cañizares et al., 2013 (link)). The 35S-dsSAHH construct was kindly provided by Dr. David M. Bisaro (The Ohio State University, Columbus, OH, USA), and plasmids 35S-GFP and 35S-p19 were kindly provided by Dr. David C. Baulcombe (University of Cambridge, Cambridge, United Kingdom).
Recombinant Expression of Arabidopsis TOP1 and TOP2
Purification of Dmr1-MBP-His6 Fusion Protein
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