Axiovision le program

After 1 day in the tanks, three mussels per day for 30 days were checked for R. pseudosericeus larvae. The presence of larvae on the four gills (left or right, outer or inner) of U. d. sinuolatus mussels was checked using a mussel‐opening device that enabled mussels to be opened to approximately 10 mm. The adductor muscle of mussels was cut to examine the position, number, and developmental stage of bitterling eggs/embryos/larvae. Mussels without bitterling eggs/embryos/larvae were housed in different tanks.
To evaluate the changes in larval position in the mussels, the gills were divided into nine parts (Figure 1); from the gill demibranch to its point of contact with the suprabranchial cavity, the gill was divided into lower part (L), middle part (M), and upper part (U); it was also divided into three parts in the other direction, 3 being the farthest from the outlet, followed by 2 and 1. Moreover, the larvae’s position was accurately recorded and photographed (Canon, Mark II, Tokyo, Japan) by measuring the transverse length of the siphon of the mussel and the longitudinal length from the suprabranchial cavity to the gill demibranch. The developmental stages of R. pseudosericeus larvae were determined under a stereoscopic microscope (Nikon, SMZ‐10, Tokyo, Japan) using AxioVision LE program (version 4.5, Carl Zeiss, Germany), as described by Kim, Kang, and Kim (2006).

E. coli, 4Q7 and HD1 were cultivated in LB or nutrient broth at 37°C or 28°C (200 rpm), respectively. Samples were taken at different times and monitored by phase contrast and fluorescence microscopy. Data were obtained using an Axio Imager A.1 Zeiss microscope with the filter set at 09, an excitation of 450–490 nm, and an emission of 515 nm. Crystal area was estimated using the AxioVision LE program (Carl Zeiss Microscopy, Göttingen Germany).