Rosa26 eyfp

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The Rosa26-EYFP is a reporter mouse line that expresses enhanced yellow fluorescent protein (EYFP) from the ubiquitously active Rosa26 locus. This allows for the detection and tracking of EYFP-expressing cells in vivo.

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Rosa26-LSL-EYFP (4 citations) Rosa26-CAG-LSL-ChR2-EYFP-WPRE (4 citations) Rosa26-LoxP-STOP-LoxP-EYFP (3 citations) ROSA26-stop-EYFP (2 citations) Ai32: ROSA26-loxStoplox-ChR2-eYFP (2 citations) Rosa26-CAG-LSL- eNpHR3.0-EYFP-WPRE (2 citations)

26 protocols using rosa26 eyfp

Transgenic Mice for Muscle Stem Cell Studies

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The mice strains were obtained from Jackson Laboratories, including C57BL/6J mice (Stock no. 000664, adult mice at 8 weeks and aged mice at 18 months), mdx mice (C57BL/10ScSn-Dmdmdx/J, Stock No: 001801), Rosa26-tdTomato (Stock no. 7909), Pax7-cre/ERT2 (Stock no. 017763), and Rosa26-EYFP (Stock no. 006148). Pax7-cre/ERT2 and Rosa26-EYFP were crossed to produce Pax7-CreER:Rosa26-EYFP offspring. The genotypes of all transgenic mice were confirmed with genotyping analyses according to the manufacturer’s instructions. All mice were bred and maintained in specific pathogen-free conditions. All animal work was conducted under protocols approved by the UC Berkeley or UCLA Animal Research Committee.

Fang J., Sia J., Soto J., Wang P., Li L.K., Hsueh Y.Y., Sun R., Francis Faull K., Tidball J.G, & Li S. (2021). Skeletal-muscle regeneration via the chemical induction and expansion of myogenic stem cells in situ or in vitro. Nature biomedical engineering, 5(8), 864-879.

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Genetically Modified Mouse Models

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C57BL/6, ROSA26^eYFP and FVB-Tg(CAG-luc,-GFP)L2G85Chco/J male mice were obtained from Jackson Laboratory. NOD/MrkBomTac-Prkdc^scid female mice were obtained from Taconic Biosciences. Pax7Cre^ER mouse were provided by Dr Charles Keller, Oregon Health and Science University, Portland, OR, USA61 (link). Tamoxifen injections for Cre recombinase activation were performed administering five doses (5 mg per mouse) every 2 days and waiting a minimum of 7 days before using the animals experimentally61 (link). To control for tamoxifen injection toxicity, we injected all mice with tamoxifen. Mice were housed and maintained in the Veterinary Medical Unit at the Veterans Affairs Palo Alto Health Care Systems. Animal protocols were approved by the Administrative Panel on Laboratory Animal Care of Stanford University.

Quarta M., Cromie M., Chacon R., Blonigan J., Garcia V., Akimenko I., Hamer M., Paine P., Stok M., Shrager J.B, & Rando T.A. (2017). Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss. Nature Communications, 8, 15613.

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Transgenic Mice for Immunological Studies

Cited 1 time

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Ccl19-Cre mice were previously described^{19 (link)}. Rosa26-EYFP (stock number 006148), Rosa26-DTR (iDTR, stock number 007900) Cd21-Cre (stock number 006368) and OT-II (stock number 004194) were purchased from Jackson Laboratory. TCR-S transgenic mice were recently described²⁴. Mice were maintained under specific pathogen-free conditions in accordance with institutional and National Institute of Health guidelines and used at 5–7 weeks of age. Experiments were conducted without blinding using sex and age matched mice for all in vivo experiments. For multiple time-point experiments, mice were randomly assigned to each group. Animal studies were approved by the Research Animal Care committee of Dana-Farber Cancer Institute.

Cremasco V., Woodruff M.C., Onder L., Cupovic J., Nieves-Bonilla J.M., Schildberg F.A., Chang J., Cremasco F., Harvey C.J., Wucherpfennig K., Ludewig B., Carroll M.C, & Turley S.J. (2014). B cell homeostasis and follicle confines are governed by fibroblastic reticular cells. Nature Immunology, 15(10), 973-981.

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Generation and Characterization of Ythdf2 Mice

Cited 2 times

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Ythdf2^fl/fl mice were reported previously (Yu et al., 2021b (link)). Six3-cre (Furuta et al., 2000 (link)), Thy1-GFP (Feng et al., 2000 (link)), and Rosa26-eYFP (Srinivas et al., 2001 (link)) mice were from Jackson Laboratory. For timed pregnancy, embryos were identified as E0.5 when a copulatory plug was observed. Genotyping primers are as following: the first Ythdf2-loxP site, 5’-GCTTGTAGTTATGTTGTGTACCAC-3’ and 5’-GCAGCTCTGACTATTCTAAAACCTCC-3’; the second Ythdf2-loxP site, 5’-CTCATAACATCCATAGCCACAGG-3’, and 5’-CCAAGAGATAGCTTTCCTAATG-3’.
Six3-cre site, 5’-CCTTCCTCCCTCTCTATGTG-3’ and 5’-GAACGAACCTGGTCGAAATC-3’.
Rosa26-eYFP wild type site, 5’-CTGGCTTCTGAGGACCG-3’ and 5’-CAGGACAACGCCCACACA-3’; the mutant site, 5’-AGGGCGAGGAGCTGTTCA-3’ and 5’-TGAAGTCGATGCCCTTCAG-3’. All experiments using mice were carried out following the animal protocols approved by the Laboratory Animal Welfare and Ethics Committee of Southern University of Science and Technology.

Niu F., Han P., Zhang J., She Y., Yang L., Yu J., Zhuang M., Tang K., Shi Y., Yang B., Liu C., Peng B, & Ji S.J. (2022). The m6A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells. eLife, 11, e75827.

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Genetically Modified Mouse Models

Cited 3 times

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Specific pathogen-free (SPF) C57BL/6J mice (#000664), Rosa26-tdTomato mice (#007914), Rosa26-eYFP (#007903), Actb-DsRed (#006051), Actb-GFP (#003291), Lgr5-Cre (#008875), and OT-II (#004194) mice were purchased from the Jackson Laboratory. Lats1^flox/flox ^{29 (link)}, Lats2^flox/flox^{30 (link)}, Yap^flox/flox^{27 (link)}, Taz^flox/flox^{28 (link)}, Ltbr^flox/flox ^{20 (link)}, Ccl19-Cre^{20 (link)}, and Pdgfrb-Cre-ER^T2 ^{45 (link)} mice were transferred, established, and bred in SPF animal facilities at KAIST. All mice were maintained in the C57BL/6 background and fed with free access to a standard diet (PMI LabDiet) and water. In order to induce Cre activity in the Cre-ER^T2 mice, 2 mg of tamoxifen (Sigma-Aldrich) was dissolved in corn oil (Sigma-Aldrich) and intra-peritoneally (i.p.) injected at indicated time points. All mice were anesthetized with i.p. injection of a combination of anesthetics (80 mg/kg ketamine and 12 mg/kg of xylazine) before being euthanatized. We complied with all ethical regulations for animal testing and research and performed all animal experiments and euthanasia under the approval from the Institute Animal Care and Use Committee (No. KA2016-12) of Korea Advanced Institute of Science and Technology (KAIST). Mouse model nomenclatures are included in Supplementary Table 1.

Choi S.Y., Bae H., Jeong S.H., Park I., Cho H., Hong S.P., Lee D.H., Lee C.K., Park J.S., Suh S.H., Choi J., Yang M.J., Jang J.Y., Onder L., Moon J.H., Jeong H.S., Adams R.H., Kim J.M., Ludewig B., Song J.H., Lim D.S, & Koh G.Y. (2020). YAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells. Nature Communications, 11, 519.

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Conditional Knockout of Dnmt3a and Dnmt3b in Mice

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Animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) and conducted in accordance with Washington University School of Medicine institutional guidelines. Mice were C57Bl/6 background, Mx1-Cre:Dnmt3a^fl/fl and Mx1-Cre:Dnmt3a^fl/fl;Dnmt3b^fl/fl mice have been described (16 ). Recombination of floxed alleles was mediated by six intraperitoneal injections (300μg/mouse) of polyinosinic-polycytidylic acid (pIpC; Sigma, St. Louis, MO, USA) in PBS every other day. The following strains were obtained from the Jackson Laboratory (Bar Harbor, ME, USA) - Lck-CRE (003802), CD8a-CRE (008766), Rosa26^NICD (008159), Rosa26^EYFP (006148) and Nr4a1^KO (006187).

Kramer A.C., Kothari A., Wilson W.C., Celik H., Nikitas J., Mallaney C., Ostrander E.L., Eultgen E., Martens A., Valentine M.C., Young A.L., Druley T.E., Figueroa M.E., Zhang B, & Challen G.A. (2017). Dnmt3a Regulates T-cell Development and Suppresses T-ALL Transformation. Leukemia, 31(11), 2479-2490.

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Transgenic Mouse Models for Neurological Research

Cited 1 time

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Cspg4-CreER (JAX stock# 008538), Plp1-CreER (JAX stock# 005975), ROSA26-tdTomato (Ai14; JAX stock# 007914), ROSA26-EYFP (JAX stock# 006148) and ROSA26-mEGFP (mT/mG; JAX stock# 007576) mice were purchased from the Jackson Laboratory. Mobp-EGFP BAC mice were generated by GENSAT and described previously (Kang et al., 2013 (link)). All mice were maintained with a 12-hour light/ 12-hour dark cycle. The genetic background of Cspg4-CreER; Ai14; R26-Gria2 Tg mice used for neonatal hypoxic-ischemic brain injury was C57BL/6. Other mice for other experiments had mixed genomic backgrounds of B6SJL, C57BL/6 and 129. Both sexes of mice were used in an unbiased manner. The ages of mice for each experiment are described in Results, timelines in figures, or figure legends. All experiments were carried out in compliance with the animal protocols approved by the Institutional Animal Care and Committee (IACUC) at Temple University School of Medicine.

Khawaja R.R., Agarwal A., Fukaya M., Jeong H.K., Gross S., Gonzalez-Fernandez E., Soboloff J., Bergles D.E, & Kang S.H. (2021). GluA2 overexpression in oligodendrocyte progenitors promotes postinjury oligodendrocyte regeneration. Cell reports, 35(7), 109147.

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Transgenic Mouse Model Characterization

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C57BL/6, ROSA26^eYFP, and B6.Cg-Foxn1nu/J mice were obtained from Jackson Laboratory. Pax7Cre^ER mouse and ROSA26^LuSEAP were provided by Dr. Charles Keller, Oregon Health and Science University, Portland, Oregon, USA. Tamoxifen injections for Cre recombinase activation were performed as described previously ^{68 (link)}. To control for tamoxifen injection toxicity, we injected all mice with tamoxifen. All experimental mice employed were 3 to 6 months old. Mice were housed and maintained in the Veterinary Medical Unit at the Veterans Affairs Palo Alto Health Care Systems. Animal protocols were approved by the Administrative Panel on Laboratory Animal Care of Stanford University.

Quarta M., Brett J.O., DiMarco R., De Morree A., Boutet S.C., Chacon R., Gibbons M.C., Garcia V.A., Su J., Shrager J.B., Heilshorn S, & Rando T.A. (2016). A bioengineered niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy. Nature biotechnology, 34(7), 752-759.

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Conditional Knockout of Parp1 in Mice

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Parp1-floxed mice were provided by Dr. Kraus (Luo et al., 2017 (link)) via an approved Material Transfer Agreement (MTA). Olig2-Cre (B6.129-Olig2^{tm1.1(cre)Wdr}/J, stock 025567), Pdgfrα-CreER^T2 (B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J, stock 018280) and Rosa26-LoxP-STOP-LoxP-EYFP (referred to as Rosa26-EYFP, B6.129X1-Gt(ROSA)26Sor^tm1(EYFP)Cos/J, stock 006148) were purchased from the Jackson Laboratory. Cre transgene was always maintained as heterozygosity. We crossed Cre lines with Parp1^fl/fl mice to generate Parp1 cKO mice. Mice that are homozygous for Parp1 KO were purchased from the Jackson Laboratory (129S-Parp1^tm1Zqw/J, stock 002779) and bred with C57BL/6J mice (stock 002779, Jackson Laboratory) to get heterozygous Parp1 KO mice. WT and Parp1 KO mice were subsequently generated by crossing female heterozygous Parp1 KO mice with male heterozygous Parp1 KO mice. Both male and female mice were used in this study. All animals were from C57BL/6 background and maintained in 12 h light/dark cycle with water and food. Animals and procedures in this study were approved by the Institutional Animal Care and Use Committee at the University of California, Davis.

Wang Y., Zhang Y., Zhang S., Kim B., Hull V.L., Xu J., Prabhu P., Gregory M., Martinez-Cerdeno V., Zhan X., Deng W, & Guo F. (2021). PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination. Cell reports, 37(1), 109695.

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Mouse Breeding and Characterization

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Mice were bred and raised at Temple University mouse facility. Wild-type C57BL/6 (Jackson Laboratories), Ki67^CreERT2 (Jackson Laboratories), Rosa26^tdTomato (Jackson Laboratories), Rosa26^EYFP (Jackson Laboratories), SPC^EGFP (Jackson Laboratories), and Sftpc^CreERT2 (7) mice were used. All experiments used 6- to 10-week-old mice. Both genders of mice were used and kept on a mixed genetic background. All the animal experiments were carried out by the NIH guidelines (Guide for the care and use of laboratory animals). All experimental procedures involving animals in this study were reviewed and approved by the Institutional Animal Care and Use Committee of Temple University Medical Center.

Ali M., LaCanna R., Lian Z., Huang J., Tan Y., Shao W., Yu X, & Tian Y. (2022). Transcriptional responses to injury of regenerative lung alveolar epithelium. iScience, 25(8), 104843.

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