Ammonium formate

Manufactured by Maclin Biochemical Technology

Sourced in China, United States

Ammonium formate is a chemical compound used as a buffer solution in various analytical and biochemical applications. It is a colorless, crystalline solid that is soluble in water. Ammonium formate is commonly used in liquid chromatography-mass spectrometry (LC-MS) as a mobile phase additive to enhance ionization and improve separation performance.

Automatically generated - may contain errors

Lab products found in correlation

7 protocols using ammonium formate

Quantitative Analysis of Flavonoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC-MS-grade methanol was purchased from Merck (Darmstadt, Germany). Formic acid was purchased from Fisher Scientific (LC-MS grade, Thermo Scientific, Carlsbad, CA, USA), and ammonium formate was obtained from Macklin (HPLC grade, purity: ≥99%). Sakuranetin was purchased from Sigma-Aldrich (Sigma-Aldrich, Inc., St. Louis, MO, USA, CAS number: 2957-21-3); quercitrin (CAS number: 522-12-3), myricitrin (CAS number: 17912-87-7), kaempferol (CAS number: 520-18-3), luteolin (CAS number: 491-70-3), rutin (CAS number: 153-18-4), hyperoside (CAS number: 482-36-0), kaempferol-3-O-rutinoside (CAS number: 17650-84-9), catechin (CAS number: 225937-10-0), and catechin gallate (CAS number: 130405-40-2) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Ultrapure water was purified using a Milli-Q system (18 MΩ*cm, Millipore, Burlington, MA, USA).

Zhou Z., Deng Z., Liang S., Zou X., Teng Y., Wang W, & Fu L. (2023). Quantitative Analysis of Flavonoids in Fruiting Bodies of Sanghuangporus Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry. Molecules, 28(13), 5166.

+ Open protocol

+ Expand

Lipidomic Analysis Using Synthetic Standards

Check if the same lab product or an alternative is used in the 5 most similar protocols

The PL standards were purchased from Avanti Polar Lipids, including phosphatidylcholine (PC 17:0/17:0), phosphatidylethanolamine (PE 17:0/17:0), phosphatidylglycerol (PG 17:0/17:0), phosphatidic acid (PA 17:0/17:0), phosphatidylinositol (PI 8:0/8:0), sphingomyelin (SM d18:1/12:0) and SPLASH LIPIDOMIX (isotopic internal standard mixture). Glyceryl trilinoleate (TAG) was purchased from Sigma Aldrich. Other chemicals, including methanol, chloroform, acetonitrile and ammonium formate, were purchased from Macklin Biochemical Technology Co., Ltd. (Shanghai, China) (high-performance liquid chromatography or analytical reagent grade).

Yao J., Zhu J., Zhao M., Zhou L, & Marchioni E. (2023). Untargeted Lipidomics Method for the Discrimination of Five Crab Species by Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry Combined with Chemometrics. Molecules, 28(9), 3653.

+ Open protocol

+ Expand

Highly Purified Compounds for Analytical Methods

Check if the same lab product or an alternative is used in the 5 most similar protocols

L-carnitine (purity >99.5%) and MILD (purity >99.6%) were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). Acetonitrile [(ACN); purity >99.9%] and formic acid [(FA); purity >99.0%] of liquid chromatography grade were obtained from Merck KGaA (Darmstadt, Germany) and Anaqua Chemical Supply (Wilmington, DE, USA), respectively. Ammonium formate (purity >99.0%) was obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Water was purified and deionized with a Milli-Q system manufactured by Millipore (Bedford, MA, USA).

Jiang W.T., Tang L.X., Pan J., Su C.J., Shen Z., Hu Z.H., Zhan Z.B, & Shi A.M. (2022). Development and validation of an UHPLC-Orbitrap-HRMS method for rapid determination of endogenous L-carnitine in patients on hemodialysis/peritoneal dialysis and its application to promote rational drug use. Annals of Translational Medicine, 10(2), 103.

+ Open protocol

+ Expand

Simultaneous Quantification of Antibiotics in Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sulbactam sodium, tazobactam, meropenem trihydrate, metronidazole, ampicillin sodium, cefotaxime sodium, cefoperazone sodium, piperacillin sodium, and acetaminophen were all purchased from Macklin (Shanghai, China). ULC–MS grade acetonitrile and methanol were supplied from the Anaqua™ Chemicals Supply (Wilmington, NC, USA). ULC–MS grade formic acid and ammonium formate were purchased from Macklin (Shanghai, China). Deionized water was purified using a Hitech Smart-S ultrapure water system (Shanghai, China). Whole blood was obtained from drug-free healthy volunteers and collected in heparin sodium tubes.

Liu Q., Liu L., Yuan Y, & Xie F. (2023). A Validated UHPLC–MS/MS Method to Quantify Eight Antibiotics in Quantitative Dried Blood Spots in Support of Pharmacokinetic Studies in Neonates. Antibiotics, 12(2), 199.

+ Open protocol

+ Expand

Synthesis of Metal Complexes and Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cadmium nitrate tetrahydrate (Cd(NO₃)₂·4H₂O), sodium sulfide nonahydrate (Na₂S·9H₂O) and potassium dichromate (K₂Cr₂O₇) were provided by Xilong Scientific Co., Ltd (Guangdong, China). p-Nitrophenol (C₆H₅NO₃), p-aminophenol (C₆H₆N₂O₂) and ammonium formate (CH₅NO₂) were supplied by Macklin Biochemical Co., Ltd (Shanghai, China). Attapulgite was purchased from Xuyi (Jiangsu, China). All the reagents were AR (analytical reagent) grade in purity. Deionized water used in all the processes was from local sources.

Ma R., Xie L., Huang Y., Zhuo K., Xu J, & Zhang Y. (2021). A facile approach to synthesize CdS–attapulgite as a photocatalyst for reduction reactions in water. RSC Advances, 11(43), 27003-27010.

+ Open protocol

+ Expand

Quantitative Analysis of Neonicotinoid Pesticides and Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

NEOs (acetamiprid, ACE; imidacloprid, IMI; nitenpyram, NIT; thiacloprid, THI; thiamethoxam, THX; clothianidin, CLO; dinotefuran, DIN; sulfoxaflor, SUL; flonicamid, FLO; flupyradifurone, FLU) and their metabolites (IMI-olefin, IMI-urea, desnitro imidacloprid, N-dNO₂-IMI; 5-hydroxy imidacloprid, 5-OH-IMI (CAS: 155802-61-2); hydroxy imidacloprid, OH-IMI (CAS: 380912-09-4); N-desmethyl-acetamiprid, N-dm-ACE; 1-methyl-3-(tetrahydro-3-furylmethyl) urea, UF; 1-methyl-3-(tetrahydro-3-furylmethyl) guanidium dihydrogen, DN; 6-chloronicotinic acid, 6-CNA (a metabolite of the chloropyridinyl neonicotinoids); 4-trifluoromethylnicotinic acid, TFNA (a metabolite of FLO)) were purchased from Alta Scientific Co., ltd. (Tianjin, China). HPLC-grade acetonitrile and formic acid were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Ammonium formate was purchased from Shanghai Macklin Biochemical Co., ltd (Shanghai, China). Primary secondary amine (PSA, 40–60 μm), bonded silica C18_, and graphitized carbon black (GCB, 120–400 mesh) were purchased from Bonna-Agela Technologies (Tianjin, China). Analytical grade salts such as anhydrous magnesium sulfate, sodium chloride, sodium acetate, and other reagents were purchased from Beijing Chemical Reagents Co. (Beijing, China). The water used in the experiment was purified using a Milli-Q system (Merck Millipore, Billerica, USA).

Wang Y., Qin J., Lu Q., Tian J., Ke T., Guo M., Luo J, & Yang M. (2023). Residue detection and correlation analysis of multiple neonicotinoid insecticides and their metabolites in edible herbs. Food Chemistry: X, 17, 100603.

+ Open protocol

+ Expand

Quantifying Phenolic Compounds in Plant Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC-MS grade methanol was purchased from Merck (Darmstadt, Germany). Formic acid was purchased from Fisher Scientific (LC-MS grade, Thermo Scientific, Carlsbad, CA, USA), and ammonium formate was purchased from Macklin (HPLC grade, purity: ≥99%). Chlorogenic acid (CAS No. 327-97-9), p-coumaric acid (CAS No. 501-98-4), caffeic acid (CAS No. 331-39-5), ferulic acid (CAS No. 537-98-4), sinapic acid (CAS No. 530-59-6), cryptoChlorogenic acid (CAS No. 905-99-7), syringic acid (CAS No. 530-57-4), and protocatechuic acid (CAS No. 99-50-3) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Ultrapure water was purified using a Milli-Q system (18 MΩ × cm, Millipore, Burlington, MA, USA).

Zhou Z., Liang S., Zou X., Teng Y., Wang W, & Fu L. (2023). Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of Sanghuangporus baumii (Pilát) L.W. Zhou and Y.C. Dai. Plants, 12(20), 3565.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!