Ab128930

Manufactured by Abcam

Sourced in United States

Ab128930 is a lab equipment product offered by Abcam. It serves as a core function without any extrapolation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Ab16049, by Abcam (1 mentions) Ab152, by Merck Group (1 mentions) Dnmt3b, by Abcam (1 mentions) Ab5084p, by Merck Group (1 mentions) Ab1591p, by Merck Group (1 mentions) Dnmt3a, by Cell Signaling Technology (1 mentions) Dnmt1, by Cell Signaling Technology (1 mentions) Ab6276, by Abcam (1 mentions) Ab15497, by Abcam (1 mentions) Ab198394, by Abcam (1 mentions) Cd437, by Merck Group (1 mentions) Antagomir, by Thermo Fisher Scientific (1 mentions) Chip grade ab290, by Abcam (1 mentions)

2 protocols using ab128930

Immunoblotting of Dopaminergic Markers

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This was performed as previously described [20 (link)] with antibodies against D2R (1:500, rabbit), dopamine transporter (DAT; 1:500, rabbit), tyrosine hydroxylase (TH; 1:1000, rabbit) (AB5084P, AB1591P and AB152, Merck Millipore, Billerica, MA, USA), signal transducer and activator of transcription 3α (STAT3α; 1:1000, rabbit), DNMT1 (1:1000, rabbit), DNMT3a (1:1000, rabbit) (nos. 8768, 5032 and 3598; Cell Signaling Technology, Tokyo, Japan), DNMT3b (1 μg/ml, rabbit), ERRγ (1:1000, rabbit) and β-actin (1:10,000, mouse) (ab16049, ab128930 and ab6276; Abcam, Cambridge, MA, USA).

Kozuka C., Kaname T., Shimizu-Okabe C., Takayama C., Tsutsui M., Matsushita M., Abe K, & Masuzaki H. (2017). Impact of brown rice-specific γ-oryzanol on epigenetic modulation of dopamine D2 receptors in brain striatum in high-fat-diet-induced obesity in mice. Diabetologia, 60(8), 1502-1511.

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Characterization of Androgen Receptor Signaling

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DHT(Sigma), CD437 (Sigma), Enza (Sigma) mir-96 mimic and antagomir (Ambion, nocodazole (CST)
For Western immunoblots the following antibodies were used; Anti-RARγ (D3A4) rabbit mAb #8965 Cell Signaling Technology (CST); Anti-Myc Tag (9B11); Anti-TACC1 mouse mAb #2276 (CST); Anti-AR-V7 rabbit mAb #ab198394 (Abcam); Anti-AR (D6F11) rabbit mAb #5153 (CST); Anti-PGC-1α (3G6) Rabbit mAb #2178 (CST); Anti-REG4 Rabbit Polyclonal Antibody #43411 (CST); Anti-PMEPA1 rabbit polyclonal antibody # 16521–1-AP; Anti-ERRg rabbit mAb (EPR8100) #ab128930 (Abcam); Anti-DLC1 rabbit polyclonal antibody #PA5–53635 (Invitrogen); anti-PCNA rabbit polyclonal antibody #ab15497 (Abcam).
For rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) assay RARγ (D3A4) #8965 CST antibody was used.
For CUT&RUN the following antibodies were used; Anti-Acetyl-Histone H3K27 (D5E4) XP^® rabbit mAb #8173 (CST); anti-GFP antibody, (ChIP Grade-ab290, Abcam); anti-AR (D6F11) XP^® rabbit mAb #5153 (CST), anti-IgG #2729S (CST), rabbit anti-H3S10ph #39254 (Active Motif).

Wani S.A., Hussain S., Gray J.S., Nayak D., Tang H., Perez L.M., Long M.D., Siddappa M., McCabe C.J., Sucheston-Campbell L.E., Freeman M.R, & Campbell M.J. (2024). Epigenetic disruption of the RARγ complex impairs its function to bookmark AR enhancer interactions required for enzalutamide sensitivity in prostate cancer. bioRxiv.

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