Oxidative damage indicator ROS content in H9C2 cells with various treatments was determined using the total ROS assay kit based on DCFH-DA staining (Beyotime, Beijing, China). Mitochondrial specific ROS was measured using the MitoSox staining kit (Beyotime, Beijing, China). DAPI was used for staining of cell nucleus. The cells were observed under inverted fluorescence microscope (Olympus, Japan).
Total ros assay kit
Manufactured by Beyotime
Sourced in China
The Total ROS Assay Kit is a laboratory tool designed to measure the total reactive oxygen species (ROS) levels in various biological samples. It provides a quantitative assessment of the cumulative ROS present, including superoxide, hydrogen peroxide, and hydroxyl radicals. The kit utilizes a fluorescent probe that reacts with ROS, generating a measurable signal proportional to the total ROS concentration. This information can be useful for researchers studying oxidative stress and related biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by Beyotime (3 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate, by Beyotime (2 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (2 mentions)
3 3 dioctadecyloxacarbocyanine perchlorate, by Beyotime (2 mentions)
Amyloid β1 40 human elisa kit, by Elabscience (2 mentions)
Amyloid β1 42 human elisa kit, by Elabscience (2 mentions)
Lysosenser yellow blue dextran, by Thermo Fisher Scientific (2 mentions)
Bca protein assay kit, by Beyotime (2 mentions)
Inverted fluorescence microscope, by Olympus (1 mentions)
Protease inhibitor cocktail, by Apexbio (1 mentions)
Oligomycin, by Merck Group (1 mentions)
Mitosox red, by Thermo Fisher Scientific (1 mentions)
Z vad fmk, by Beyotime (1 mentions)
Pierce bcatm protein assay kit, by Thermo Fisher Scientific (1 mentions)
Carbonyl cyanide 4 trifluoromethoxy phenylhydrazone fccp, by Merck Group (1 mentions)
2 deoxyglucose 2 dg, by Merck Group (1 mentions)
Tunel apoptosis assay kit, by Beyotime (1 mentions)
Antimycin a, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Crystal violet solution, by Beyotime (1 mentions)
4 protocols using total ros assay kit
1
Quantifying Oxidative Stress in H9C2 Cells
2
Comprehensive Cellular Assays for Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell Counting Kit-8 (CCK-8), Total ROS Assay Kit, Crystal violet solution and N-acetyl-L-cysteine (NAC), Z-VAD-FMK and TUNEL Apoptosis Assay Kit (C1091) were purchased from Beyotime Biotechnology (Shanghai, China); Antimycin A, Rotenone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), Oligomycin, 2-Deoxyglucose (2-DG) and L-glutamine were purchased from Sigma-Aldrich; CPX was from Dibo Chemical Technology (Shanghai, China); Annexin V-FITC/PI Apoptosis Double Dye Detection Kit was obtained from BD Biosciences; the Pierce BCATM protein assay kit, MitoSOXTM Red and Enhanced chemiluminescence (ECL) were from Thermo Fisher Scientific; Protease inhibitor cocktail was purchased from APExBIO, Houston, TX, USA); DNeasy Blood & Tissue Kit (Cat. No.: 69504) was from QIAGEN.
3
Amyloid-beta Inhibition and Cell Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rapamycin (Rapa), coumarin-6 (Cou-6), and okadaic acid (OA) were purchased from Sigma–Aldrich Inc. (St.Louis, MO, USA). Gypenoside XVII (GP-17) was attained from Push Biotechnology Co., Ltd. (Chengdu, China). RP-1 (CAPDTKTQ) and Aβ1–42 were synthesized by Guotai Biotechnology Co., Ltd. (Hefei, China) and Chinese Peptide Co., Ltd. (Hangzhou, China), respectively. 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO), Cell Counting Kit-8 (CCK-8), Total ROS assay kit and BCA Protein Assay Kit were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Amyloid-β1–40 Human ELISA Kit and Amyloid-β1–42 Human ELISA Kit were purchased from Elabscience Biotechnology Co., Ltd. (Shanghai, China). Lysotracker Red DND-99 and Lysosenser Yellow/Blue dextran were purchased from Thermo Fisher Scientific, Inc. (Eugene, OR, USA). The mouse brain microvascular endothelial cells (bEnd.3), mouse hippocampal neuronal cells (HT22), and murine neonatal microglial cells (BV2) were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). All chemicals used were analytical or reagent grade.
4
Amyloid-beta Inhibition and Cell Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rapamycin (Rapa), coumarin-6 (Cou-6), and okadaic acid (OA) were purchased from Sigma–Aldrich Inc. (St.Louis, MO, USA). Gypenoside XVII (GP-17) was attained from Push Biotechnology Co., Ltd. (Chengdu, China). RP-1 (CAPDTKTQ) and Aβ1–42 were synthesized by Guotai Biotechnology Co., Ltd. (Hefei, China) and Chinese Peptide Co., Ltd. (Hangzhou, China), respectively. 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO), Cell Counting Kit-8 (CCK-8), Total ROS assay kit and BCA Protein Assay Kit were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Amyloid-β1–40 Human ELISA Kit and Amyloid-β1–42 Human ELISA Kit were purchased from Elabscience Biotechnology Co., Ltd. (Shanghai, China). Lysotracker Red DND-99 and Lysosenser Yellow/Blue dextran were purchased from Thermo Fisher Scientific, Inc. (Eugene, OR, USA). The mouse brain microvascular endothelial cells (bEnd.3), mouse hippocampal neuronal cells (HT22), and murine neonatal microglial cells (BV2) were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). All chemicals used were analytical or reagent grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!