For IGF-1 measurement, blood was collected by retro-orbital puncture under 5% urethane anaesthesia after 6-h fasting. Plasma IGF-1 levels were measured by using a mouse IGF-1 ELISA kit (Alpco, Salem, NH). For pituitary hormones measurement, a single adenohypophysis was lysed in 100 μl RIPA buffer and diluted by PBS to 1 ml. Anterior pituitary hormones were measured with corresponding mouse ELISA kits (Alpco).
Mouse elisa kit
Manufactured by ALPCO
Sourced in United States
The Mouse ELISA Kit is a quantitative assay designed to measure the concentration of a specific analyte in mouse biological samples. It utilizes the Enzyme-Linked Immunosorbent Assay (ELISA) technique to detect and quantify the target analyte. The kit provides the necessary reagents and materials to perform the analysis.
Automatically generated - may contain errors
Lab products found in correlation
Insuman rapid, by Sanofi (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Ilab 600 analyzer, by Werfen (2 mentions)
D glucose, by Merck Group (2 mentions)
Mouse igf 1 elisa kit, by ALPCO (1 mentions)
Accu chek, by Roche (1 mentions)
One touch 2 glucose meter, by LifeScan (1 mentions)
Elisa kit, by Merck Group (1 mentions)
Dxc 800 analyzer, by Beckman Coulter (1 mentions)
Akta fplc system, by Cytiva (1 mentions)
Adiponectin, by Crystal Chem (1 mentions)
Glucose, by B. Braun (1 mentions)
Novolin, by Novo Nordisk (1 mentions)
Cobas 6000 analyzer, by Roche (1 mentions)
11 protocols using mouse elisa kit
1
Plasma IGF-1 and Pituitary Hormone Assays
2
Evaluation of Glucose Homeostasis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oral glucose tolerance tests (OGTTs) were performed in overnight-fasted mice at 10 weeks of age by administration of 2 g/kg glucose by gavage. Insulin tolerance was assessed in 5-h-fasted animals after administration of 0.6 units/kg i.p. human insulin at 12 weeks of age. Insulin secretion in vivo was measured using hyperglycemic clamps. Mice underwent catheterization of the right jugular vein under general anesthesia. After a 5-day recovery, conscious mice were subjected to one-step hyperglycemic clamps. A 20% dextrose solution (McKesson Canada, Montreal, QC, Canada) was infused to clamp blood glucose at ∼22 mmol/L for 60–80 min (ACCU-CHEK; Roche, Indianapolis, IN). Plasma samples were collected from the tail for measurements of insulin and C-peptide using mouse ELISA kits (Alpco Diagnostics, Salem, NH). The insulin sensitivity index (M/I) was calculated as the glucose infusion rate (M) divided by the average insulinemia during the last 30 min of the clamp (I). The disposition index (DI), an index of β-cell function taking into account the prevailing level of insulin sensitivity developed in humans (35 (link)) and validated in rodents (36 (link)), was calculated by multiplying M/I by C-peptide levels during the last 30 min of the clamp.
3
Metabolic Biomarkers in Animal Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood glucose levels were determined with the One Touch II glucose meter (LifeScan). Serum insulin levels were measured with a commercially available enzyme-linked immunosorbent assay (ELISA) (ALPCO, Salem, NH). Serum levels of total and high-molecular-weight (HMW) adiponectin were measured using mouse ELISA kits (ALPCO). Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), and leptin were measured with commercially available ELISA kits (Millipore, Billerica, MA). Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined with the Beckman DXC 800 analyzer (Brea, CA). Hepatic lipid peroxidation was determined using a commercially available kit (Cayman Chemicals, Ann Arbor, MI). Lipid peroxidation was assessed as the amount of thiobarbituric acid reactive substances (TBARS) produced according to manufacturer's instruction.
4
Insulin Extraction from Mouse Islets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ten equal-sized islets per mouse were incubated in 30 μl acid/ethanol (75% ethanol and 0.15 M HCl) at 4 °C overnight with gentle rotation to extract insulin and then centrifuged at 14,000 rpm for 10 min. The supernatant was diluted at 1:50 ratio, and the insulin content was measured by the mouse ELISA kit (ALPCO) and normalized to the total islet DNA content.
5
Metabolic Profiling in Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma triglyceride and cholesterol concentrations were measured by using Biochemistry Analyzer MultiCare (Biochemical Systems International-Srl, Arezzo, Italy). Plasma glucose levels were measured using a commercial glucometer (GlucoMen LX meter, Menarini, Italy) in blood collected from the tail vein. Plasma insulin was quantified by a mouse ELISA kit (Alpco diagnostics, Salem, NH, USA). Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) were carried out in mice fasted overnight. For IPGTT, the animals were injected intraperitoneally (i.p.) with glucose (2 g/kg body weight) in 0.9% saline. For ITT, mice were given an i.p. injection of insulin (0.5 U/kg body weight) (Insuman Rapid, Sanofi Aventis, Italy) in 0.9% saline. Blood glucose was measured at different time intervals (0, 15, 30, 60, 120 min from the administrations). The Homeostasis Model Assessment of basal Insulin Resistance (HOMA-IR) was calculated as the product of fasting insulin (ng/mL) and fasting glucose (mg/dL) divided by the constant 22.5.
6
Perifusion Assay for Insulin Secretion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The isolated islets were left to recover overnight at 37 °C (5% CO2) in the RPMI 1640 medium (Gibco) containing 10% fetal bovine serum. Then, groups of 50 islets per mouse were placed in a dynamic perifusion system (Amersham Biosciences AKTA FPLC System). The perifusion was performed using Krebs buffer with 2.8 mM glucose at a flow rate of 1 ml/min for 15 min to establish stable basal insulin secretion. Next, the islets were perifused with 2.8 mM glucose for 10 min, and fractions of 500 μl were collected every 30 s. Then, the glucose concentration was increased to 20 mM, and fractions of 500 μl were collected every 30 s for 20 min. Finally, the islets were perifused with 30 mM KCl, and fractions of (500 μl) were collected every 30 s for 10 min. After the perifusion, the islets were recollected from the column for genomic DNA measurement. The insulin in the effluent was measured by a mouse ELISA kit (ALPCO). The fractional insulin secretion rate was calculated as secreted insulin per minute normalized to the DNA content.
7
Glucose and Lipid Metabolism Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma triglyceride, total cholesterol, HDL and LDL concentrations were measured using the ILAB 600 Analyzer (Instrumentation Laboratory, Bedford, MA, USA). Fasting blood glucose concentrations were determined by a glucometer (GlucoMen LX meter, Menarini, Florence, Italy). Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) were carried out in overnight fasting mice. For IPGTT, mice were injected intraperitoneally (i.p.) with glucose (2 g/kg b.w.) (D-glucose, Sigma-Aldrich, Milan, Italy) in 0.9% saline. For ITT, mice were injected i.p. with insulin (0.5 U/kg b.w.) (Insuman Rapid, Sanofi Aventis, Italy) in 0.9% saline. Glucose concentrations were measured at different time intervals (0, 15, 30, 60, 120 min) by tail vein. Plasma insulin was quantified using a mouse ELISA kit (Alpco diagnostics, Salem, NH, USA) according to the manufacturer’s instructions. The HOMA-IR index was calculated as the product of fasting insulin (ng/mL) and fasting glucose (mg/dL) divided by the constant 22.5.
ELISA kits were used to determine plasma leptin (Life Technologies, Frederick, MD, USA) and adiponectin (Crystal Chem, Elk Grove Village, IL, USA).
ELISA kits were used to determine plasma leptin (Life Technologies, Frederick, MD, USA) and adiponectin (Crystal Chem, Elk Grove Village, IL, USA).
8
Glucose and Insulin Homeostasis Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma triglyceride and total cholesterol were measured using the ILAB 600 Analyzer (Instrumentation Laboratory, Bedford, MA, USA). Fasting blood glucose concentrations were determined by a glucometer (GlucoMen LX meter, Menarini, Florence, Italy). Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) were carried out in overnight-fasting mice. For IPGTT, mice were injected intraperitoneally (i.p.) with glucose (2 g/kg b.w.) (D-glucose, Sigma-Aldrich, Milan, Italy) in 0.9% saline. For ITT, mice were injected i.p. with insulin (0.5 U/kg b.w.) (Insuman Rapid, Sanofi Aventis, Italy) in 0.9% saline. Tail-vein-measured glucose concentrations were taken at different time points (0, 15, 30, 60, and 120 min). Plasma insulin was quantified using a mouse ELISA kit (Alpco diagnostics, Salem, NH, USA) according to the manufacturer’s instructions, and the HOMA-IR, index of insulin resistance, was calculated as the ratio of fasting insulin (ng/mL) and fasting glucose (mg/dL) divided by the constant 22.5.
9
Glucose and Insulin Tolerance Tests
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For intraperitoneal glucose tolerance tests, mice were fasted 12 h overnight and injected i.p. with glucose (40%; B.Braun, Melsungen, Germany) at a dose of 1 g/kg body weight. Blood samples were obtained at time points 0, 15, 30, 60, 90, and 120 min for glucose measurements by using a Glucometer. For i.p. insulin tolerance tests, mice were injected with 0.75 U/kg body weight recombinant human insulin (Novolin, Novo Nordisk) after 4–5-h fasting, and glucose concentration was determined with the Glucometer. Insulin secretion was measured before (0 min) and after (15 and 30 min) i.p. injection of glucose (2 g/kg) and measured by using ultrasensitive mouse Elisa kit (ALPCO Diagnostics, Salem, NH).
10
Insulin Secretion Dynamics in Isolated Islets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The isolated islets were left to recover overnight at 37 °C (5% CO2) in the RPMI 1640 medium (Gibco) containing 10% fetal bovine serum. Then, groups of 30 islets per mouse were incubated in 2.8 mM glucose for 30 min at 37 °C to establish stable basal insulin secretion and then washed by Krebs buffer twice. The islets were transferred into a new well containing 2 ml of 2.8 mM glucose solution for 30 min at 37 °C, and 100-μl media was collected for time point 1 and then transferred into a new well containing 2 ml of 20 mM glucose solution for 30 min at 37 °C, and 100 μl media was collected for time point 2. The islets were then recovered for genomic DNA measurement. Insulin levels in the collected media were measured by the mouse ELISA kit (ALPCO) and normalized to the DNA content.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!