Binding of FH to spirochetes was also assessed by ELISA. Briefly, bacterial cells (2 × 107 cells) in 100 μl PBS were immobilized to Nunc MaxiSorp 96-well microtiter plates at 4°C overnight and binding of FH was detected as described above.
Nunc maxisorp 96 well microtiter plates
The Nunc MaxiSorp 96-well microtiter plates are a type of laboratory equipment designed for various immunoassay applications. They feature a high-affinity surface that allows for the effective binding of proteins and other biomolecules.
Lab products found in correlation
3 protocols using nunc maxisorp 96 well microtiter plates
Quantification of FH Binding to Spirochetes
Binding of FH to spirochetes was also assessed by ELISA. Briefly, bacterial cells (2 × 107 cells) in 100 μl PBS were immobilized to Nunc MaxiSorp 96-well microtiter plates at 4°C overnight and binding of FH was detected as described above.
Quantifying Complement Protein Binding
To determine dose-dependency and to calculate the dissociation constant, CipA was immobilized (5 µg/ml) and incubated with increasing amounts (0 to 50 nM) of C3b, C5, and FI, respectively. The antigen-antibody complexes were detected by using the appropriate antibodies as described above.
Antibody Complement Deposition Assay
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!