Sr analysis

Pre-pulse inhibition of the acoustic startle response was measured with SR-LAB equipment and software from San Diego Instruments. Accelerometers were calibrated to 700 ± 5 mV and output voltages were amplified and analyzed for voltage changes using SR Analysis (San Diego Instruments, San Diego, CA, USA), exported as an excel file and statistically analyzed on SPSS v19 (IBM, Armonk, NY, USA). Background white noise was maintained at 65 dB. PPI was measured in a 30 min test with 80 randomized trials of: pulse alone (100 dB above background), pre-pulse alone (4, 8, or 16d B above background), pre-pulse plus pulse and no pulse. Five pulse alone trials were performed at the start and end of the 80 trials. The onset of the pulse following the pre-pulse was delayed 100 ms. Time interval between trials were randomized from 5s-20s. Pre-pulse inhibition was measured as a decrease in the amplitude of startle response to a 100 dB acoustic startle pulse, following each pre-pulse (4, 8, and 16 dB). Control diet: n = WT 7M, 10F; NR1 6M, 8F. Deficient diet: n = WT 12M, 11F; NR1 8M, 12F. Supplemented diet: n = WT 13M, 17F; NR1 18M, 18F.

A prepulse inhibition test was performed to measure abnormalities in sensorimotor gating or startle response in OGG1 mice (~5 months old) [31 (link)]. The prepulse inhibition test was conducted using SR-LAB equipment and SR-LAB software from San Diego Instruments. Accelerometers were calibrated to 700  ±  5 mV and output voltages were amplified and analyzed for voltage changes using SR Analysis (San Diego Instruments, San Diego, CA, USA). Each mouse was tested for 30 min. The background white noise was maintained at 65 dB, and each mouse was subjected to 80 randomized trials of pulse alone (100 dB above background), prepulse alone (4, 8 or 16 dB above background), prepulse plus pulse and no pulse, with five pulse-alone trials performed at the start and end of the 80 trials. Time intervals between trials were randomized from 5 s to 20 s, with a delay of 100 ms between the prepulse and the pulse. Prepulse inhibition was thus measured as a decrease in the amplitude of startle response to a 100 dB (above background) acoustic startle pulse following each prepulse (4, 8 and 16 dB above background).