Polyperoxidase anti mouse igg

Manufactured by ZSGB-BIO

Sourced in China

Polyperoxidase-anti-mouse IgG is a secondary antibody conjugate used for the detection of mouse primary antibodies in various immunoassays. It is composed of a polymer backbone with multiple peroxidase enzymes and anti-mouse IgG antibodies. This conjugate amplifies the signal for improved sensitivity in immunodetection applications.

Automatically generated - may contain errors

Lab products found in correlation

3 3 diaminobenzidine dab, by ZSGB-BIO (2 mentions) Anti nephrin antibody, by Santa Cruz Biotechnology (1 mentions) Dm60008 microscope, by Leica (1 mentions) Image pro plus 6, by Media Cybernetics (1 mentions) Dm60008, by Leica (1 mentions) Ab47092, by Abcam (1 mentions) L proline, by Merck Group (1 mentions) L ascorbic acid 2 phosphate, by Merck Group (1 mentions) Tgf 1, by Thermo Fisher Scientific (1 mentions) Non essential amino acid (neaa), by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 affinipure goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Anti bmp2 ab6285, by Abcam (1 mentions) Its premix, by Merck Group (1 mentions) Dnase 1, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Alizarin red, by Solarbio (1 mentions) Blyscan gag assay kit, by Biocolor (1 mentions) Tritc labeled phalloidin, by Yeasen (1 mentions)

4 protocols using polyperoxidase anti mouse igg

Immunohistochemical Analysis of Kidney Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Expressions of fibronectin, collagen I, α-SMA, and E-cadherin in the kidney tissues of mice were determined by immunohistochemistry. Paraffin-embedded kidney tissue sections were deparaffinized, rehydrated, and subjected to microwave-based antigen retrieval in citrate buffer. A blocking step was performed using 10% normal goat serum. The kidney sections were incubated overnight at 4 °C with anti-fibronectin, collagen I, α-SMA, and E-cadherin primary antibodies, and with polyperoxidase-anti-mouse IgG (ZSGB-BIO, Beijng, China). Immunoreactive signals were developed upon incubation with 3,3-diaminobenzidine (DAB, ZSGB-BIO). Images of fibronectin, collagen I, α-SMA, and E-cadherin were obtained and photographed under a microscope (Olympus Corporation, Tokyo, Japan) at 200× magnification.

Dong Z., Sun Y., Wei G., Li S, & Zhao Z. (2019). A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy. Molecules, 24(22), 4119.

+ Open protocol

+ Expand

Nephrin Immunohistochemical Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The paraffin sections were processed via routine dewaxing and hydration. Antigen retrieval was performed with a microwave, and non-specific antibody binding sites were blocked with 3% H₂O₂ (ZSGB-BIO, Beijing, China) for 10 min. The samples were then incubated with an anti-nephrin antibody (1:500, Santa Cruz Biotech, Santa Cruz, CA, USA) at 4°C overnight, and with polyperoxidase-anti-mouse IgG (ZSGB-BIO, Beijng, China). Immunoreactive signals were developed upon incubation with 3,3-diaminobenzidine (DAB, ZSGB-BIO). The sections were observed with the Leica DM60008 microscope at 400× magnifications, and the quantitative analysis of the immunohistochemical images was done with the Image-Pro plus 6.0 software (Media Cybernetics, Rockville, MD, USA).

Tian N., Gao Y., Wang X., Wu X., Zou D., Zhu Z., Han Z., Wang T, & Shi Y. (2018). Emodin mitigates podocytes apoptosis induced by endoplasmic reticulum stress through the inhibition of the PERK pathway in diabetic nephropathy. Drug Design, Development and Therapy, 12, 2195-2211.

+ Open protocol

+ Expand

Immunohistochemical Analysis of ASC Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The paraffin-embedded sections were processed via routine dewaxing and hydration. In brief, sections were heated in a microwave for antigen retrieval and blocked with 3% H₂O₂ (ZSGB-BIO, Beijing, China) for 10 min to avoid nonspecific binding. The sections were subsequently incubated with anti-ASC antibody (1 : 100, ab47092, abcam, UK) at 4°C overnight and reacted with poly peroxidase-anti-mouse IgG (ZSGB-BIO, Beijng, China). Afterwards, 3,3-diaminobenzidine (DAB, ZSGB-BIO) was used for visualization of immunoreactive signals. Finally, the sections were observed under a microscope (Leica DM60008) at 400x magnifications.

Wang T., Gao Y., Yue R., Wang X., Shi Y., Xu J., Wu B, & Li Y. (2020). Ginsenoside Rg1 Alleviates Podocyte Injury Induced by Hyperlipidemia via Targeting the mTOR/NF-κB/NLRP3 Axis. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 2735714.

+ Open protocol

+ Expand

Collagen Extraction and Stem Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Collagen type I was extracted from a new born calf skin and dissolved in a 0.5M acetic acid solution. Alpha-modified Eagle's Medium (-MEM) and Dulbecco's Modified Eagle Medium (DMEM) were purchased from Hyclone. 4'-6-diamidino-2phenylindole (DAPI) was obtained from Beyotime Biotechnology, China. DNAse I, L-ascorbic acid 2-phosphate, Lproline, Dexamethasone, ITS+Premix was purchased from Sigma-Aldrich, USA. Nonessential amino acid (NEAA) and Fetal Bovine Serum (FBS) were obtained from Gibco, USA. TGF-1 was obtained from PeproTech, USA. Fluorescein diacetate (FDA), propidium iodide (PI), Triton X-100 and alizarin red were obtained from Solarbio, China. TRITC-labeled phalloidin was purchased from YEASEN Bio, China. Anti-TGF-1 antibody (ab190503) and anti-BMP2 (ab6285) were purchased from Abcam, USA. Anti-IGF1 (nbp2-34361), anti-VEGF (nbp2-45235), anti-PEDF (mab1177), anti-COL1 (nb600-450), anti-COL2 (nbp2-33343) antibodies were obtained from NOVUS, USA. Alexa Fluor488-affiniPure Goat Anti-Mouse IgG was purchased from Jackson ImmunoResearch, USA. Goat serum and polyperoxidase-anti mouse IgG were obtained from ZSGB-BIO, China. Blyscan GAG assay kit was obtained from Biocolor, UK. All other chemicals were purchased from Chengdu Kelong unless otherwise specified.

Liu J., Wang X., Lu G., Tang J.Z., Wang Y., Zhang B., Sun Y., Lin H., Wang Q., Liang J., Fan Y, & Zhang X. (2019). Bionic cartilage acellular matrix microspheres as a scaffold for engineering cartilage. Journal of materials chemistry. B, 7(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!