The basal medium consisted of Advanced DMEM/F12 (Thermo fisher Scientific) supplemented with HEPES (10 mM, Thermo fisher Scientific), GlutaMAX (1x, Thermo fisher Scientific), and Penicillin-Streptomycin-Glutamine (1x, Thermo fisher Scientific).
The organoid culture medium, which was used for the organoid culture of human prostate cancer, consisted of the basal medium supplemented with nicotinamide (10 mM, Sigma), N-acetylcysteine (1 mM, Wako), A-83-01 (500 nM, Wako), B-27 (1x, Gibco), recombinant human EGF (50 ng/mL, Sigma), gastrin I human (10 nM, Peprotech), recombinant human Noggin (100 ng/mL, Peprotech), recombinant human R-spondin 1 protein (500 ng/mL, R&D), SB-202190 (10 μM, Chemscene), and 10% Afamin/Wnt3a CM (MBL) (Table S1
The organoid culture medium, which was used for the organoid culture of human prostate cancer, consisted of the basal medium supplemented with nicotinamide (10 mM, Sigma), N-acetylcysteine (1 mM, Wako), A-83-01 (500 nM, Wako), B-27 (1x, Gibco), recombinant human EGF (50 ng/mL, Sigma), gastrin I human (10 nM, Peprotech), recombinant human Noggin (100 ng/mL, Peprotech), recombinant human R-spondin 1 protein (500 ng/mL, R&D), SB-202190 (10 μM, Chemscene), and 10% Afamin/Wnt3a CM (MBL) (