ETAR IHC performed as previously described. After deparaffinization, 4 µm paraffin sections of ovarium were incubated with 3% H2O2 in PBS for 5 minutes to reduce the endogenous peroxidase. Therefore, sections were incubated with anti-ETAR antibody (1:100) (SC33535, Santa Cruz) at 4°C for overnight and followed with EnVision+System+HRP anti-rabbit secondary antibody (K4002, Dako) (1:200) for 60 minutes at room temperature. Finally, immune-positive cells were visualized by incubating with 3,3’-diaminobenzidine. Hematoxylin staining used for counterstaining the sections.
Envision system hrp anti rabbit secondary antibody
Manufactured by Agilent Technologies
The EnVision+System+HRP anti-rabbit secondary antibody is a laboratory equipment product designed for use in immunoassays. It functions as a detection reagent, providing a means to amplify and visualize the signal generated by primary antibodies that recognize target antigens. The product is based on horseradish peroxidase (HRP) conjugated to an anti-rabbit secondary antibody, enabling the detection of rabbit-derived primary antibodies.
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Lab products found in correlation
2 protocols using envision system hrp anti rabbit secondary antibody
1
Immunohistochemistry for ETAR in Ovarium
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ETAR IHC performed as previously described. After deparaffinization, 4 µm paraffin sections of ovarium were incubated with 3% H2O2 in PBS for 5 minutes to reduce the endogenous peroxidase. Therefore, sections were incubated with anti-ETAR antibody (1:100) (SC33535, Santa Cruz) at 4°C for overnight and followed with EnVision+System+HRP anti-rabbit secondary antibody (K4002, Dako) (1:200) for 60 minutes at room temperature. Finally, immune-positive cells were visualized by incubating with 3,3’-diaminobenzidine. Hematoxylin staining used for counterstaining the sections.
+ Expand
ETAR IHC performed as previously described. After deparaffinization, 4 µm paraffin sections of ovarium were incubated with 3% H2O2 in PBS for 5 minutes to reduce the endogenous peroxidase. Therefore, sections were incubated with anti-ETAR antibody (1:100) (SC33535, Santa Cruz) at 4°C for overnight and followed with EnVision+System+HRP anti-rabbit secondary antibody (K4002, Dako) (1:200) for 60 minutes at room temperature. Finally, immune-positive cells were visualized by incubating with 3,3’-diaminobenzidine. Hematoxylin staining used for counterstaining the sections.
2
DMBA-Induced Tumor Model Evaluation
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7,12-dimethylbenz(a)anthracene (DMBA) and CIS were obtained from Sigma (USA). CUR was obtained from Plamed Green Science Limited (China). CIS working solution was prepared in Phosphate Buffer Saline (PBS) for cell culture or in normal saline for in vivo study. CUR was suspended in DMSO for in vitro or carboxymethyl cellulose for in vivo analysis. All the solutions freshly prepared before the experiment. ETAR antibody was purchased from Santa Cruz, and EnVision+System+HRP anti-rabbit secondary antibody was purchased from Dako.
+ Expand
7,12-dimethylbenz(a)anthracene (DMBA) and CIS were obtained from Sigma (USA). CUR was obtained from Plamed Green Science Limited (China). CIS working solution was prepared in Phosphate Buffer Saline (PBS) for cell culture or in normal saline for in vivo study. CUR was suspended in DMSO for in vitro or carboxymethyl cellulose for in vivo analysis. All the solutions freshly prepared before the experiment. ETAR antibody was purchased from Santa Cruz, and EnVision+System+HRP anti-rabbit secondary antibody was purchased from Dako.
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