Fluorescent conjugated antibodies

Manufactured by BioLegend

Sourced in United States

Fluorescent-conjugated antibodies are laboratory reagents used in various analytical techniques, such as flow cytometry and immunofluorescence microscopy. These antibodies are produced by chemically linking a fluorescent dye molecule to the surface of an antibody, which allows for the detection and visualization of target molecules or cells within a sample. The fluorescent signal emitted by the conjugated antibody can be measured and analyzed to provide information about the presence, abundance, and characteristics of the targeted analytes.

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Lab products found in correlation

Fcr blocking reagent, by Miltenyi Biotec (2 mentions) Allophycocyanin apc anti human lineage cocktail, by BioLegend (2 mentions) Fluorescein isothiocyanate fitc anti human cd34 antibodies, by BioLegend (2 mentions) Accuri c6 cytometer, by BD (2 mentions) Roswell park memorial institute (rpmi), by Corning (1 mentions) Fetal bovine serum (fbs), by Equitech-Bio (1 mentions) Macoy s 5a modified medium, by Merck Group (1 mentions) Eagle s minimal essential medium, by Corning (1 mentions) Penicillin streptomycin stock, by Lonza (1 mentions) Anti human cd45, by BioLegend (1 mentions) Isotype control antibody, by BioLegend (1 mentions)

Spelling variants of this product

Fluorescent conjugated primary antibodies (2 citations) Fluorescent dye-conjugated antibodies (2 citations)

3 protocols using fluorescent conjugated antibodies

Colon Cancer Cell Lines and Reagents

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The mouse colon carcinoma cell line CT26.CL25 (CRL-2639); human colon carcinoma
cell lines SW620 (CCL-227), HT29 (HTB-38), COLO 205 (CCL-222), and Caco-2
(HTB-37); and human lymphoblast cell line Jurkat (TIB-152) were purchased from
American Type Culture Collection (ATCC; Manassas, VA). RPMI (Roswell Park
Memorial Institute) 1640 and Eagle’s minimal essential medium (MEM) was
purchased from Mediatech, Inc (Manassas, VA). Macoy’s 5A modified medium was
from Sigma (St Louis, MO). Fetal bovine serum was from EQUITECH-BIO Inc
(Kerrville, TX). Penicillin-streptomycin stock was obtained from Lonza Rockland,
Inc (Allendale, NJ). Lipopolysaccharides (LPS) from Escherichia
coli O111:B6 were purchased from Sigma. Fluorescent conjugated
antibodies targeting CD4 (H129.19), CD8b (YTS156.7.7), CD19 (6D5), dendritic
cells (DCs) marker (33D1), LY6G (1A8), CD68 (FA-11), and mouse IgG
(immunoglobulin G) isotype were obtained from BioLegend (San Diego, CA).

Ishiguro S., Robben N., Burghart R., Cote P., Greenway S., Thakkar R., Upreti D., Nakashima A., Suzuki K., Comer J, & Tamura M. (2020). Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice. Integrative Cancer Therapies, 19, 1534735419900555.

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Quantifying Hematopoietic Stem Cells

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Flow cytometry was carried out by suspending MNCs in the stain buffer and treated first with FcR blocking reagent (1:100, Miltenyi Biotech) followed by incubation with the following fluorescent-conjugated antibodies (Biolegend), allophycocyanin (APC) anti-human lineage cocktail (1:500, Biolegend), phycoerythrin (PE) anti-human CD45 (1:500) and Fluorescein isothiocyanate (FITC) anti-human CD34 antibodies (1 in 250) or isotype control antibodies (1 in 500) (Biolegend, San Diego, CA, U.S.A.) for 45 min at 4°C. Dead cells were excluded using 7-AAD viability staining solution. Flow cytometry was carried out by using C6 Accuri cytometer (BD).

Joshi S., Montes de Oca I., Maghrabi A., Lopez-Yang C., Quiroz-Olvera J., Garcia C.A, & Jarajapu Y.P. (2021). ACE2 gene transfer ameliorates vasoreparative dysfunction in CD34+ cells derived from diabetic older adults. Clinical Science (London, England : 1979), 135(2), 367-385.

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Flow Cytometry Characterization of MNCs

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Flow cytometry was carried out by suspending MNCs in the stain buffer and treated first with FcR blocking reagent (1:100, Miltenyi Biotec) followed by incubation with the following fluorescent-conjugated antibodies (Biolegend), allophycocyanin (APC) anti-human lineage cocktail (1:500, Biolegend), phycoerythrin (PE) anti-human CD45 (1:500) and fluorescein isothiocyanate (FITC) anti-human CD34 antibodies (1 in 250), or isotype control antibodies (1 in 500) (Biolegend, SanDiego, CA, USA) for 45 min at 4 °C. Dead cells were excluded using the 7-AAD viability staining solution. Flow cytometry was carried out by using the C6 Accuri cytometer (BD).

Joshi S., Chittimalli K., Jahan J., Vasam G, & Jarajapu Y.P. (2020). ACE2/ACE imbalance and impaired vasoreparative functions of stem/progenitor cells in aging. GeroScience, 43(3), 1423-1436.

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