The SH-SY5Y (San Diego, CA, USA); GAPDH, anti-PI3K, anti-mTOR, anti-COX-2, anti-iNOS, and anti-GSK3β (Wuhan Sanying Biotechnology Co., Ltd., Wuhan, China); DMEM (Gibco, Carlsbad, CA, USA); RNA extraction kit, reverse transcription kit, and RT-PCR kit (Invitrogen, Carlsbad, CA, USA); primer synthesis (Takara, Dalian, China); and protein quantitative kit and cell lysate (Biyuntian Biotechnology Research Institute, Nantong City, China) were purchased. ELISA kits were purchased from Westang Technology Ltd. (Shanghai, China).
Primer synthesis
Manufactured by Takara Bio
Sourced in United States, China
Primer synthesis is a laboratory technique used to produce short, synthetic DNA sequences called primers. Primers are essential components in various molecular biology applications, such as polymerase chain reaction (PCR), DNA sequencing, and gene expression analysis. The core function of primer synthesis is to generate specific DNA fragments that can be used to initiate and amplify target genetic regions.
Automatically generated - may contain errors
Lab products found in correlation
Rt pcr kit, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Zerumbone, by Merck Group (1 mentions)
Gapdh, by Wuhan Sanying Biotechnology (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Reverse transcription polymerase chain reaction rt pcr kits, by Thermo Fisher Scientific (1 mentions)
Sybr greener qpcr supermix universal kit, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Ki67 antibody, by Merck Group (1 mentions)
Claudin 1 antibody, by Abcam (1 mentions)
Phospho p44 42 mapk erk1 2 antibody, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
P44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions)
Occludin antibody, by Abcam (1 mentions)
Phospho nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
Caspase 3 antibody, by Cell Signaling Technology (1 mentions)
6 protocols using primer synthesis
1
SH-SY5Y Cell Line Analysis
2
Zerumbone Inhibits Esophageal Cancer
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Esophageal cancer EC-109 cells (Cell Bank of Chinese Academy of Sciences, Shanghai, China); zerumbone (Sigma, New York, NY, USA); methyl thiazolyl tetrazolium (MTT) (Sigma, St. Louis, MO, USA); rabbit anti-human P53 and Bcl-2 poluclonal primary antibodies and goat anti-rabbit horseradish peroxidase (HRP)-labeled secondary polyclonal antibody (cat. nos. 10442-1-AP, 12789-1-AP and SA00001-2; Wuhan Sanying Biotechnology, Wuhan, China; Dulbecco's modified Eagle's medium (DMEM) (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); TdT-mediated dUTP nick end-labeling (TUNEL) apoptosis kit, TRIzol and reverse transcription-polymerase chain reaction (RT-PCR) kits (Invitrogen; Thermo Fisher Scientific, Inc.); primer synthesis (Takara, Dalian, China).
3
BV2 Cell-Based Inflammation Study
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The following reagents were used: The BV2 cells (San Diego, CA, U.S.A.); GAPDH, anti-PTX3-1, anti-TLR4, anti-COX-2, anti-iNOS and anti-p65 (Wuhan Sanying Biotechnology Co., Ltd., Wuhan, China); DMEM (Gibco, Carlsbad, CA, U.S.A.); RNA extraction kit, reverse transcription kit, RT-PCR Kit (Invitrogen, Carlsbad, CA, U.S.A.), primer synthesis (Takara, Dalian, China), protein quantitative kit, cell lysate (Biyuntian Biotechnology Research Institute, Nantong City, China).
4
Colon Cancer Cell Line SW480 Assay
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Aesculetin, bromodeoxyuridine (BrdU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, St. Louis, MO, USA); human colon cancer cell line SW480 (Cell Bank, Chinese Academy of Sciences, Shanghai, China); BrdU kit, rabbit anti-human glyceraldehyde 3-phosphate dehydrogenase (GAPDH; cat. no. 10494-1-AP), β-catenin (cat. no. 17565-1-AP), c-Myc (cat. no. 10828-1-AP) and cyclin D1 (cat. no. 60186-1-Ig) primary polyclonal antibodies and goat anti-rabbit horseradish peroxidase (HRP)-labeled secondary polyclonal antibody (cat. no. SA00001-2) (ProteinTech Group Inc., Wuhan, China); Dulbecco's modified Eagle's medium (DMEM; Gibco, Carlsbad, CA, USA); ribonucleic acid (RNA) extraction kits, reverse transcription kits, reverse transcription-polymerase chain reaction (RT-PCR) kits (Invitrogen, Carlsbad, CA, USA); primer synthesis (Takara, Dalian, China); and human immunization (Biotechnology Research Institute, Nantong, China), bicinchoninic acid (BCA) protein quantification kits and cell lysate (Beyotime Institute of Biotechnology, Nantong, China) were used in the present study.
5
Evaluating β-bourbonene's Effects on PC-3M Prostate Cancer
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β-bourbonene (Sigma; Merck KGaA, Darmstadt, Germany); prostate cancer cell line PC-3M (Cell Bank of CAS, Shanghai, China); Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); Cell Counting Kit-8 (CCK-8) (Sigma; Merck KGaA); primary antibodies and horseradish peroxidase (HRP)-labeled secondary antibodies of Bax, Bcl-2 (Proteintech Group, Inc., Wuhan, China); primer synthesis (Takara Biotechnology Co., Ltd., Dalian, China), TRIzol and SYBR-GreenER™ qPCR SuperMix Universal kits (Invitrogen; Thermo Fisher Scientific, Inc.).
6
Traditional Chinese Medicine Enema for Colitis
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DSS (AppliChem, Lot#：A3261‐0250 Germany); the six medicinal herbs of QBD raw powder, as shown in Table 1 (Pharmacy Department, The Second Hospital affiliated to Shanxi University of TCM, Xianyang, China) were decoting up 150 mL by traditional decocting method for enema； Mesalazine Enemas (V Vifor AG Zweigniederlassung Medichemie Ettingen， H20150127, Switzerland); faecal occult blood (FOB) (Baso Diagnostics Inc， Zhuhai， china); NF‐κB p65 antibody (Cell Signaling, #8242, Danvers, MA, USA); Phospho‐NF‐κB p65 antibody (Cell Signaling, #3039); p44/42 MAPK (ERK1/2) (antibody (Cell Signaling，#4695); Phospho‐p44/42 MAPK (ERK1/2) antibody (Cell Signaling, #4370); MMP‐9 antibody (Merck, AB19016, Burlington, MA, USA); Ki67 antibody (Merck, AB9260); Cleaved Caspase‐3 antibody (Cell Signaling, #9664); Caspase‐3 antibody (Cell Signaling, #9662); β‐Actin antibody (Cell Signaling,# 4970) Notch1 antibody (Cell Signaling, #3608s); ZO‐1 antibody (abcam, ab96587, Cambridge, MA, USA); Occludin antibody (abcam, ab 21632); claudin‐1 antibody (abcam, ab15098); primer synthesis (Takara Bio Inc, Dalian, China).
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