Sham and ovariectomy surgeries were conducted in 3 month old rats. The surgery was performed under 2.5% isoflurane anesthesia (Patterson Veterinary, Greeley, CO) at 1 L/min oxygen (Roberts Oxygen Company Inc, Rockville, MD). Bilateral flank incisions were made. In the Ovx group, the ovaries were removed, while in the Sham group, the ovaries were exposed without excising them. Then, the muscle layers and skin were individually sutured. All rats received the analgesic carprofen (5 mg/kg (Rimadyl); Zoetis; Parsippany, NJ) subcutaneously after surgery and up to 72 h when needed.
Isoflurane anesthesia
Manufactured by Performance Health
Sourced in United States
Isoflurane is a halogenated ether compound used as an inhalational anesthetic agent. It is a clear, colorless, and volatile liquid that produces a state of unconsciousness and relaxation when inhaled. Isoflurane is primarily used in veterinary medicine to induce and maintain general anesthesia in various animal species.
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Lab products found in correlation
4 protocols using isoflurane anesthesia
1
Surgical Menopause Model in Rats
2
Isoflurane Anesthesia Protocol
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Isoflurane anesthesia was obtained from Patterson Veterinary (Greeley, CO, USA). Sterile and filtered phosphate buffered saline (PBS, 1X) was obtained from GE Life Sciences (Pittsburgh, PA, USA). Unless otherwise noted, all other reagents were obtained through VWR (Radnor, PA, USA).
3
Ovariectomy and Sham Surgeries in Rats
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Sham and ovariectomy surgeries were conducted in 3 month old rats. The surgery was performed under 2.5% isoflurane anesthesia (Patterson Veterinary, Greeley, CO) at 1 L/min oxygen (Roberts Oxygen Company Inc, Rockville, MD). Bilateral flank incisions were made. In the Ovx group, the ovaries were removed, while in the Sham group, the ovaries were exposed without excising them. Then, the muscle layers and skin were individually sutured. All rats received the analgesic carprofen (5 mg/kg (Rimadyl); Zoetis; Parsippany, NJ) subcutaneously after surgery and up to 72 h when needed.
4
Isoflurane Anesthesia Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isoflurane anesthesia was obtained from Patterson Veterinary (Greeley, CO, USA). Sterile and filtered phosphate buffered saline (PBS, 1X) was obtained from GE Life Sciences (Pittsburgh, PA, USA). Unless otherwise noted, all other reagents were obtained through VWR (Radnor, PA, USA).
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