Lung tissue was homogenized in radioimmunoprecipitation assay (RIPA) buffer (Fudebio, Hangzhou, China) containing protease inhibitors (PMSF, Beyotime Technology, Nanjing, China). Protein concentration was quantified by using a bicinchoninic acid (BCA) method (Beyotime Technology, Nanjing, China). For Western blot, the samples were separated by sodium dodecyl sulfate (SDS)-12% polyacrylamide gel electrophoresis (PAGE) and electroblotted onto polyvinylidene difluoride (PVDF) membrane (Millipore, USA). The membranes were subsequently blocked in 5% non-fat milk in TBST and probed with primary antibodies against IL-1β (Abclonal, Wuhan, China), IL-6 (Huabio, Hangzhou, China) and TNF-ɑ (Santa Cruz, Texas, USA) at a final dilution of 1:1000 overnight at 4°C, then immunoblotted with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (Fudebio, Hangzhou, China). The immunoreactive bands were visualized by electrochemiluminescent (ECL).
Interleukin 6 (il 6)
Manufactured by Huabio
Sourced in China, United Kingdom
The IL-6 is a laboratory equipment designed for the detection and quantification of the cytokine interleukin-6 (IL-6) in biological samples. The core function of this product is to provide accurate and reliable measurements of IL-6 levels, which is a key biomarker used in various research and clinical applications.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (3 mentions)
Tnf α, by Abcam (2 mentions)
Polyvinylidene difluoride pvdf membrane, by Merck Group (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions)
Bca method, by Beyotime (1 mentions)
Ripa buffer, by Fudebio (1 mentions)
Anti mouse igg hrp linked secondary antibody, by Cell Signaling Technology (1 mentions)
Pro prep protein extraction solution, by iNtRON Biotechnology (1 mentions)
β actin, by Merck Group (1 mentions)
Il 1β, by MyBioSource (1 mentions)
Color prestained protein marker, by Thermo Fisher Scientific (1 mentions)
F4 80, by Huabio (1 mentions)
P nf κb, by Cell Signaling Technology (1 mentions)
P ikbα, by Abcam (1 mentions)
Il 6 elisa kit, by Solarbio (1 mentions)
Dmem culture medium, by Thermo Fisher Scientific (1 mentions)
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Nf κb, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
3 protocols using interleukin 6 (il 6)
1
Western Blot Analysis of Inflammatory Markers
2
Renal Cortical Protein Extraction and Western Blot Analysis
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Total protein was extracted from renal cortical tissues using the PRO‐PREP Protein Extraction Solution (iNtRON Biotechnology, Gyeonggi‐Do, Republic of Korea), according to the manufacturer's instructions. Western blot analysis was performed using the following antibodies: angiopoietin‐1 (Proteintech, Rosemont, IL, USA; 1:2000), angiopoietin‐2 (Proteintech, Rosemont, IL, USA; 1:2000), TNF‐α (Abcam, Cambridge, UK; 1:500), IL‐6 (Huabio, MA, USA; 1:2000), IL‐1β (MyBioSource, CA, USA; 1:3000), β‐actin (Sigma, St Louis, MO, USA; 1:10000), anti‐rabbit IgG HRP‐linked secondary antibody (Cell Signaling Technology, MA, USA; 1:2000), and anti‐mouse IgG HRP‐linked secondary antibody (Cell Signaling Technology, MA, USA; 1:2000).
3
Inflammatory Response Regulation via NF-κB Signaling
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The following reagents were used for examination: lipopolysaccharide (LPS) (Sigma, L3129-10MG), capsaicin (MCE, 404864–50 mg), FeCl3•6H2O (Aladdin, F102739-55 g), polyvinylpyrrolidone (PVP) (Aladdin, P434440-250 g), fetal bovine serum (Gibco, 30044333), DMEM culture medium (Gibco, C11995500BT), PBS (Gibco, 8123157), primary and secondary antibody dilution solution (BOSTER, 17K28C17), color pre-stained protein marker (Thermo, 26619), TNF-α (Abcam, ab66579), iNOS (Abcam, ab283655), TGF-β (Huabio, HA7211430), p-NF-κB (Cell Signaling Technology, 3033S), NF-κB (Cell Signaling Technology, 8242T), p-IKBα (Abcam, ab32518), IKBα (Abcam, ab133462), IL-6 (Huabio, R1412-2), β-actin (Cell Signaling Technology, 4970S), Pierce TM BCA protein Assay kit (Thermo Fisher Scientific, Prod # 23227), F4/80 (Huabio, RT1212), anti-rabbit IgG, HRP-linked antibody (Cell Signaling Technology, 7074S), anti-mouse IgG, HRP-linked antibody (Cell Signaling Technology, 7076S), secondary antibody (Thermo Fisher Scientific, Prod # A-31572), secondary antibody (Thermo Fisher Scientific, Prod # A-21202), SuleLumia ECL kit (Abbkine, K22020), TNF-α and iNOS ELISA kit (FineTest, EM0183, EM0272), IL-6 ELISA kit (Solarbio, SEKM-0007), Cell Counting kit-8 (CCK-8, Beyotime, C0038).
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