Sequencing libraries (25 pM) were chemically denatured and applied to an Illumina HiSeq v4 paired end flow cell using an Illumina cBot. Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4-cBot. Following transfer of the flow cell to an Illumina HiSeq 2500 instrument (HCS v2.2.38 and RTA v1.18.61), a 125 cycle paired-end sequence run was performed using HiSeq SBS Kit v4 sequencing reagents.
Hiseq sbs kit v4 sequencing
Manufactured by Illumina
The HiSeq SBS Kit v4 is a sequencing reagent kit designed for use with Illumina's HiSeq sequencing systems. The kit provides the necessary reagents for performing sequencing-by-synthesis (SBS) chemistry, which is the core function of the HiSeq platforms.
Automatically generated - may contain errors
2 protocols using hiseq sbs kit v4 sequencing
1
Illumina HiSeq 2500 Paired-End Sequencing
2
Paired-end Sequencing on Illumina HiSeq
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Sequencing libraries (25 pM) were chemically denatured and applied to an Illumina HiSeq v4 paired end flow cell using an Illumina cBot. Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4‐cBot. Following transfer of the flowcell to an Illumina HiSeq 2500 instrument (HCS v2.2.38 and RTA v1.18.61), a 125 cycle paired‐end sequence run was performed using HiSeq SBS Kit v4 sequencing reagents.
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