We detected apoptosis using the Dead End Fluorometric TUNEL System (Promega), according to the manufacturer's instructions. Four frozen hearts per group were sliced into 5 μm thick sections using a freezing microtome. Frozen sections were counterstained with mouse monoclonal α-sarcomeric actin antibody (1 : 75, Abcam), and Alexa Fluor 594 rabbit anti-mouse antibody (1 : 1000, Invitrogen) was applied as secondary antibody; cell nuclei were stained with 4′,6-Diamidino-2-Phenylindole, Dilactate (DAPI; Sigma). The stained sections were mounted and analyzed with a Fluoview 1000 confocal microscope (Olympus, Japan). We counted 6 random 400x fields and the number of TUNEL-positive cardiomyocyte nuclei was manually determined. The total number of nuclei (exhibited as DAPI-positive signals) was automatically calculated using Image-Pro Plus (version 5.0).
Alexa fluor 594 rabbit anti mouse antibody
Manufactured by Thermo Fisher Scientific
The Alexa Fluor 594 rabbit anti-mouse antibody is a fluorescent-labeled secondary antibody used for detection and visualization in immunological techniques. It is produced in rabbit and specifically targets mouse primary antibodies. The Alexa Fluor 594 dye provides a bright red fluorescent signal that can be detected using appropriate instrumentation.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole dilactate dapi, by Merck Group (1 mentions)
Mouse monoclonal α sarcomeric actin antibody, by Abcam (1 mentions)
Fluoview 1000 confocal microscope, by Olympus (1 mentions)
Deadend fluorometric tunel system, by Promega (1 mentions)
Alexa fluor 594 goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 anti mouse antibody, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 anti mouse antibody, by Thermo Fisher Scientific (1 mentions)
2 protocols using alexa fluor 594 rabbit anti mouse antibody
1
Quantifying Cardiomyocyte Apoptosis via TUNEL Assay
2
Antibody Characterization for Cell Cycle Analysis
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Antibodies specific to Cdh1 (DH01), Aurora A, and Plk1 were purchased from Abcam (Cambridge, MA). Antibodies specific to cyclin A2 (sc-751), cyclin (sc-752), cyclin D (sc-753), cyclin E (sc-247), p27 (sc-528), and Cdc20 (sc-8358) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies specific to α-tubulin (DM1α) were purchased from Sigma-Aldrich. The Skp2 antibody (32-3300), Alexa Fluor 488 anti-mouse antibody (A21202), Alexa Fluor 488 anti-rabbit antibody (A21206), Alexa Fluor 647 anti-mouse antibody (A31571), Alexa Fluor 750 anti-rabbit antibody (A21039), Alex Fluor 680 anti-mouse antibody (A21057), Alexa Fluor 594 rabbit anti-mouse antibody (A11062), Alexa Fluor 488 chicken anti-rat antibody (A21470), Alexa Fluor 594 goat anti-rabbit antibody (A11037), and Alexa Fluor 594 goat anti-chicken (A11039) were purchased from Invitrogen. IRDye 800CW anti-mouse antibody (926-32210) and IRDye 680LT anti-rabbit antibody (926-68021) were purchased from LI-COR Biosciences (Lincoln, NE). Mouse anti-BrdU antibody (347580) was purchased from Becton Dickinson (San Jose, CA). Rat anti-BrdU antibody (OBT0030) was purchased from the Accurate Chemical & Scientific Corporation (Westbury, NY).
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