Pe cd195

Manufactured by BD

The PE-CD195 is a laboratory equipment product designed for use in scientific research and analysis. It serves as a tool for the detection and measurement of specific cellular components or markers. The core function of the PE-CD195 is to facilitate the identification and quantification of these cellular targets.

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Lab products found in correlation

Lsr fortessa cytometer, by BD (2 mentions) Live dead fixable aqua stain fluorescence, by Thermo Fisher Scientific (2 mentions) Cd4 alexafluor700, by BD (1 mentions) Cd8 apc h7, by BD (1 mentions) Cd69 pe cy7, by BD (1 mentions) Hla dr fitc, by BD (1 mentions) Fitc ki 67, by BD (1 mentions)

2 protocols using pe cd195

Isolation and Phenotyping of Mucosal Mononuclear Cells

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Mucosal mononuclear cells (MMC) were isolated from rectal biopsies using a combination of mechanical and enzyme digestion ^{[26 ]}. Flow cytometric analysis was performed on a BD™ LSRFortessa cytometer (BD Biosciences, San Jose, CA). All antibodies were purchased from BD Biosciences, San Jose, CA (PerCP-CD45, Clone 2D1; Pacific Blue-CD3, Clone UCHT1; Alexa Fluor 700-CD4, Clone RPA-T4; APC-H7-CD8, Clone SK1; PE-Cy7-CD69, Clone FN50; FITC-HLA-DR, Clone L243; PE-CF594-CD38, Clone HIT2; FITC-Ki-67; APC-CD184 (CXCR4), Clone 12G5; and PE-CD195 (CCR5), Clone 2D7). Cells were stained with LIVE/DEAD® Fixable Aqua stain fluorescence (Life Technologies, Eugene, OR) to define viable cells.

McGOWAN I., WILKIN T., LANDOVITZ R.J., WU C., CHEN Y., MARZINKE M.A., HENDRIX C.W., RICHARDSON P., ESHLEMAN S.H., ANDRADE A., CHEGE W., ANDERSON P.L., McCAULEY M., FARLEY J., MAYER K.H., ANTON P., BRAND R.M., CRANSTON R.D, & GULICK R. (2019). The Pharmacokinetics, Pharmacodynamics, and Mucosal Responses to Maraviroc-Containing PrEP Regimens in Men who have Sex with Men. AIDS (London, England), 33(2), 237-246.

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Rectal Mucosal Mononuclear Cell Isolation

Cited 1 time

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Mucosal mononuclear cells (MMC) were isolated from rectal biopsies using a combination of mechanical and enzyme digestion as previously described [10 (link)]. Flow cytometric analysis was performed on a BD LSRFortessa cytometer (BD Biosciences, San Jose, CA). All data were stored in list mode and analyzed with BD FACSDIVA operating system and Flow Jo (Tree Star, Inc., Ashland, OR). All antibodies were purchased from BD Biosciences, San Jose, CA (PerCP-CD45, Clone 2D1; Pacific Blue-CD3, Clone UCHT1; PE-Cy7-CD4, Clone SK3; APC-H7-CD8, Clone SK1; FITC-CD69, Clone FN50; APC-CD184 (CXCR4), Clone 12G5 and PE-CD195 (CCR5)) and titrated under assay conditions to determine an optimum saturating dilution. Cells were stained with LIVE/DEAD Fixable Aqua stain fluorescence (Life Technologies, Eugene, OR) to define viable cells. The gating strategy used in this study can be found in S1 Fig.

Mcgowan I., Cranston R.D., Duffill K., Siegel A., Engstrom J.C., Nikiforov A., Jacobson C., Rehman K.K., Elliott J., Khanukhova E., Abebe K., Mauck C., Spiegel H.M., Dezzutti C.S., Rohan L.C., Marzinke M.A., Hiruy H., Hendrix C.W., Richardson-Harman N, & Anton P.A. (2015). A Phase 1 Randomized, Open Label, Rectal Safety, Acceptability, Pharmacokinetic, and Pharmacodynamic Study of Three Formulations of Tenofovir 1% Gel (the CHARM-01 Study). PLoS ONE, 10(5), e0125363.

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